Swimming training attenuates pancreatic apoptosis through miR-34a/Sirtu in1/P53 Axis in high-fat diet and Streptozotocin-induced Type-2 diabetic rats

Alipour, Mohammad Reza; Naderi, Roya; Alihemmati, Alireza; Sheervalilou, Roghayeh; Ghiasi, Rafighe

doi:10.1007/s40200-020-00670-6

Cited by 11 publications

(8 citation statements)

References 56 publications

(64 reference statements)

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“…In some studies, the level of P53 expression is increased, 32 , 33 but others reported a decreased in P53 after exercise. 34 , 35 However, in the present study, the level of P53 remained unchanged compared to pre-exercise leves, which is in line with the obervations of Alipour et al 36 and Sadeghi- Tabas et al 37 One of the reasons that cytochrome C and P53 expression remained unchanged in the present study was that the subjects in this study were healthy young rats. However, previous studies used old, ovariectomized, or diabetic rats, and the rate of apoptosis and cell death in these subjects are higher due to the oxidative stress and inflammatory factors, compared to the healthy young subjects.…”

Section: Discussionsupporting

confidence: 90%

The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart

Abdollahi-Diba

Bashiri

Pourmanaf

et al. 2022

J Cardiovasc Thorac Res

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Introduction: Considering the effect of Apoptosis on cardiovascular disease, this study aimed to determine the combined effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in the myocardium of male rats. Methods: A total of 35 male rats were randomly divided into five groups (n=7) as follows: endurance exercise+rosehip extract supplementation (Ex+Supp), endurance exercise (Ex), rosehip extract supplementation (Supp), six-month control (Con2), and three-month control (Con). The subjects in Ex+Supp and Ex groups performed endurance exercise (running on a treadmill at 24-33 m/min for 10-60 min) for 12 weeks, five times a week. Subjects in Ex+Supp and Supp groups consumed 1000 milligrams/ kilogram of rosehip extract for 12 weeks. Also, Con and Con2 groups did not receive any intervention. To RNA extraction and synthesis cDNA and evaluate the P53 and cytochrome C genes of the myocardium of rats, RT-PCR analysis was used. Results: Neither endurance exercise nor rosehip alone nor together significantly affected the expression of cytochrome C and P53 genes in the heart muscle of male rats (P˃ 0.05). Also, endurance exercise (P=0.001) and rosehip supplementation (P=0.002) alone and in interaction (P<0.01) had a significant effect on body weight, myocardium weight, and the ratio of myocardium weight to body weight in male rats. Conclusion: Twelve weeks of endurance exercise accompanied with rosehip extract did not significantly affect the expression of P53 and cytochrome C genes. Further studies are suggested to confirm these results.

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Section: Discussionsupporting

confidence: 90%

The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart

Abdollahi-Diba

Bashiri

Pourmanaf

et al. 2022

J Cardiovasc Thorac Res

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“…Alveolar cells that are large 22 . The content of this plant from the Karo tribe is responsible for lung tissue that begins to improve as a result of excessive swimming 23,24 .…”

Section: Discussionmentioning

confidence: 99%

“…Although intrinsic and extrinsic apoptosis has different beginnings, both of them conclude with the activation of the caspase family of cysteine proteases 23 . Caspases (such as caspase 8 or 9) start a cascade of proteolytic events that result in the activation of executioner caspases 24,25 . Numerous substrates are broken down by caspases, which also cause DNA breakage, phosphatidylserine exposure and organelle enzymatic destruction 25 .…”

Section: Discussionmentioning

confidence: 99%

Analysis of Apoptotic Cells and Lung Inflammation after Given by Vitis gracilis

Wasnis¹,

Hutahaean²,

Silaban³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: Herbs have the potential to be used in molecular therapy to treat inflammation and apoptosis. It has been demonstrated that the Indonesian native Vitris gracilis inhibits cytochrome c as a precursor to apoptosis. When body tissues are injured, infected with bacteria, exposed to toxins or exposed to heat that causes cell death, inflammation occurs. The current study aimed to look for the effect of Vitis gracilis extract in the lung histology of mice. Materials and Methods: Five treatment groups were used for the experiments, which were conducted inside the study. The T1 is the negative control, T2 is swimming mice with 0.2 mg kgG 1 b.wt., of vitamin C, T3 is swimming mice with 50 mg kgG 1 b.wt., of Vitis gracilis, T4 is swimming mice with 75 mg kgG 1 b.wt., of Vitis gracilis and T5 is swimming mice with 100 mg kgG 1 b.wt., of Vitis gracilis. The dislocation procedure was used to dissect mice, ketamine was administered before dissection and the lungs were removed for TUNEL assay examination. Results: There were no significant differences in inflammatory cells or index-positive apoptotic cells between the T1, T2 and T5 groups (p>0.05). The T3 group had the highest value, while the T1 group had the lowest. The highest dose of Vitis gracilis reduced lung cell inflammation while also improving histological structure, resulting in intact, nucleated and complete alveolar membranes with proportional endothelial cells. Conclusion: Vitis gracilis 100 mg kgG 1 b.wt., can repair and reduce inflammation in lung tissue. As a result, the higher the dose of Vitis gracilis, the less cell apoptosis occurs in the lungs.

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“…Alipour et al investigated the decrease in miR-34 in response to physical exercise; they showed for the first time the negative effect of swimming exercise on the expression of miR-34 on pancreatic tissue of mice with type 2 diabetes; in general, these findings displayed the modulation through physical activity on molecular axis miR-34/SIRT1/P53 [68]. Previous studies demonstrated that SIRT1 increased in response to physical activity, causing protective anti-apoptotic markers.…”

Section: Physical Activity and Mirnas Modulation In Prevent Of Bone L...mentioning

confidence: 99%

Physical Activity and Post-Transcriptional Regulation of Aging Decay: Modulation of Pathways in Postmenopausal Osteoporosis

et al. 2022

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Background and Objectives: Bones and the skeletal muscle play a key role in human physiology as regulators of metabolism in the whole organism. Bone tissue is identified as a complex and dynamic living unit that could react to physical activity. Hormones, growth factors, signaling factors, and environmental factors control osteogenesis, and it could be regulated at a post-transcriptional level. MicroRNAs (miRNAs) can interfere with mRNAs translation. Increasing data suggest that miRNAs, through different pathways, are involved in the regulation of bone marrow mesenchymal stem cells (BMSCs) differentiation and physical activity-induced bone remodeling. The purpose of this narrative review is to investigate the potential protective role played by physical activity in affecting miRNAs expression in close tissues and elaborate on the complex network of interplay that could drive various metabolic responses of the bone to physical activity. Materials and Methods: A bibliographic search of the scientific literature was carried out in scientific databases to investigate the possible effect of physical activity on age-related features detected in the musculoskeletal system. Results: Several studies suggested that the musculoskeletal system interacting at a biomolecular level could establish crosstalk between bone and muscle in an endocrine or paracrine way through myokines released by muscle at the periosteal interface or in the bloodstream, such as irisin. Mechanical stimuli have a key role in bone formation and resorption, increasing osteogenesis and downregulating adipogenesis of BMSC via regulation of expression of runt-related transcription factor 2 (Runx2) and peroxisome proliferator-activated receptor gamma (PPARγ), respectively. Conclusions: Increasing data suggest that miRNAs, through different pathways, are involved in the regulation of BMSCs differentiation and physical activity-induced bone remodeling. Modulation of miRNAs following physical exercise represents an interesting field of investigation since these non-coding RNAs may be considered defenders against degenerative diseases and as well as useful prognostic markers in skeletal and muscle-skeletal diseases, such as osteoporosis.

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Swimming training attenuates pancreatic apoptosis through miR-34a/Sirtu in1/P53 Axis in high-fat diet and Streptozotocin-induced Type-2 diabetic rats

Cited by 11 publications

References 56 publications

The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart

The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart

Analysis of Apoptotic Cells and Lung Inflammation after Given by Vitis gracilis

Physical Activity and Post-Transcriptional Regulation of Aging Decay: Modulation of Pathways in Postmenopausal Osteoporosis

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