Swimming Physical Training Prevented the Onset of Acute Muscle Pain by a Mechanism Dependent of PPARγ Receptors and CINC-1

Azambuja, Graciana de; Gomes, Bpfa; Messias, Leonardo Henrique Dalcheco; Aquino, Bruna de Melo; Jorge, Carolina Ocanha; Manchado-Gobatto, Fúlvia de Barros; Oliveira, Maria Cláudia G.

doi:10.1016/j.neuroscience.2019.12.017

Cited by 6 publications

(3 citation statements)

References 105 publications

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“…We have recently demonstrated that P2X3 receptors expressed on gastrocnemius muscle and spinal cord dorsal horn seem to be essential to the development of the acute mechanical muscle hyperalgesia induced by static contraction of the gastrocnemius muscle [26]. It is important to point out that the long-term static contraction produces musculoskeletal pain by similar mechanisms to carrageenan, such as final inflammatory mediators and neutrophil migration [14,27,28].…”

Section: Discussionmentioning

confidence: 99%

P2X3 receptors contribute to transition from acute to chronic muscle pain

Jorge

Azambuja

Gomes

et al. 2020

Purinergic Signalling

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This study aimed to evaluate whether the development and/or maintenance of chronic-latent muscle hyperalgesia is modulated by P2X3 receptors. We also evaluate the expression of P2X3 receptors and PKCε of dorsal root ganglions during these processes. A mouse model of chronic-latent muscle hyperalgesia, induced by carrageenan and evidenced by PGE 2 , was used. Mechanical muscle hyperalgesia was measured by Randall-Selitto analgesimeter. The involvement of P2X3 receptors was analyzed by using the selective P2X3 receptors antagonist A-317491 by intramuscular or intrathecal injections. Expression of P2X3 and PKCε in dorsal root ganglion (L4-S1) were evaluated by Western blotting. Intrathecal blockade of P2X3 receptors previously to carrageenan prevented the development and maintenance of acute and chronic-latent muscle hyperalgesia, while intramuscular blockade of P2X3 receptors previously to carrageenan only reduced the acute muscle hyperalgesia and had no effect on chronic-latent muscle hyperalgesia. Intrathecal, but not intramuscular, blockade of P2X3 receptors immediately before PGE 2 , in animals previously sensitized by carrageenan, reversed the chronic-latent muscle hyperalgesia. There was an increase in total and phosphorylated PKCε 48 h after the beginning of acute muscle hyperalgesia, and in P2X3 receptors at the period of chronic muscle hyperalgesia. P2X3 receptors expressed on spinal cord dorsal horn contribute to transition from acute to chronic muscle pain. We also suggest an interaction of PKCε and P2X3 receptors in this process. Therefore, we point out P2X3 receptors of the spinal cord dorsal horn as a pharmacological target to prevent the development or reverse the chronic muscle pain conditions.

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Section: Discussionmentioning

confidence: 99%

P2X3 receptors contribute to transition from acute to chronic muscle pain

Jorge

Azambuja

Gomes

et al. 2020

Purinergic Signalling

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“…PPARγ exerts antioxidant and anti-inflammatory effects by blocking the MAPK/NF-κB. Activation of PPARγ promotes macrophage polarization toward M2 and increases the secretion of myokines, cytokines, and chemokines ( Kim et al, 2017 ; de Azambuja et al, 2020 ). Pharmacological and immunochemical experiments confirm that exercise inhibits the development of persistent inflammatory pain in rodents through activating PPARγ and its downstream pathways ( de Azambuja et al, 2021 ).…”

Section: Mechanisms By Which Exercise Reduces Ipmentioning

confidence: 99%

Potential mechanisms of exercise for relieving inflammatory pain: a literature review of animal studies

Wu,

Song,

Zhang

et al. 2024

Front. Aging Neurosci.

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Inflammatory pain (IP) is one of the most prevalent and intractable human conditions, and it leads to progressive dysfunction and reduced quality of life. Additionally, IP is incredibly challenging to treat successfully with drugs or surgery. The development of IP is complex and multifactorial, and peripheral and central sensitization may influence chronicity and treatment resistance in IP. Understanding the mechanisms underlying IP is vital for developing novel therapies. Strong evidence suggests that exercise can be a first-line relief for patients with IP during rehabilitation. However, the mechanisms through which exercise improves IP remain unclear. Here, we reviewed the current animal experimental evidence for an exercise intervention in IP and proposed biological mechanisms for the effects of synaptic plasticity in the anterior cingulate cortex, endocannabinoids, spinal dorsal horn excitability balance, immune cell polarization balance, cytokines, and glial cells. This information will contribute to basic science and strengthen the scientific basis for exercise therapy prescriptions for IP in clinical practice.

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“…Thus, exercise can be one of the preventive approaches for chronic musculoskeletal pain conditions. Basic studies of animal models have demonstrated the effect of exercise on reducing some pain conditions and their biological mechanisms [ 5 – 9 ]. For example, a regular wheel-running exercise before a chronic constriction injury prevented the full development of allodynia; the accumulation of M1 macrophages was suppressed and that of M2 macrophages was increased at the injury site by exercise [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Regular walking exercise prior to knee osteoarthritis reduces joint pain in an animal model

Sakamoto

Miyahara

Motokawa³

et al. 2023

PLoS ONE

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We investigated the effect of regular walking exercise prior to knee osteoarthritis (OA) on pain and synovitis in a rat monoiodoacetic acid (MIA)-induced knee OA model. Seventy-one male Wistar rats were divided into three groups: (i) Sedentary + OA, (ii) Exercise + OA, and (iii) Sedentary + Sham groups. The Exercise + OA group underwent a regular treadmill walking exercise at 10 m/min (60 min/day, 5 days/week) for 6 weeks, followed by a 2-mg MIA injection in the right knee. The right knee joint was removed from rats in this group at the end of the 6-week exercise period and at 1 and 6 weeks after the MIA injection. After the 6 weeks of treadmill exercise but before MIA injection, there were no significant differences among the three groups in the pressure pain threshold, whereas at 1 week post-injection, the Exercise + OA group’s pressure pain threshold was significantly higher than that in the Sedentary + OA group, and this difference persisted until the end of the experimental period. The histological changes in articular cartilage and subchondral bone revealed by toluidine blue staining showed no difference between the Sedentary + OA and EX + OA groups. The expression levels of interleukin (IL)-4 and IL-10 mRNA in the infrapatellar fat pad and synovium were significantly increased by the treadmill exercise. Significant reductions in the number of CD68-, CD11c-positive cells and IL-1β mRNA expression and an increase in the number of CD206-positive cells were observed at 1 week after the MIA injection in the Exercise + OA group compared to the Sedentary + OA group. These results suggest that regular walking exercise prior to the development of OA could alleviate joint pain through increases in the expressions of anti-inflammatory cytokines in the rat infrapatellar fat pad and synovium.

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Swimming Physical Training Prevented the Onset of Acute Muscle Pain by a Mechanism Dependent of PPARγ Receptors and CINC-1

Cited by 6 publications

References 105 publications

P2X3 receptors contribute to transition from acute to chronic muscle pain

P2X3 receptors contribute to transition from acute to chronic muscle pain

Potential mechanisms of exercise for relieving inflammatory pain: a literature review of animal studies

Regular walking exercise prior to knee osteoarthritis reduces joint pain in an animal model

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