Swimming force and behavior of optically trapped micro-organisms

Armstrong, Declan; Nieminen, Timo A.; Stilgoe, Alexander B.; Kashchuk, Anatolii V.; Lenton, Isaac C. D.; Rubinsztein‐Dunlop, Halina

doi:10.1364/optica.394232

Cited by 22 publications

(14 citation statements)

References 26 publications

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“…To acquire both radial and axial force measurements, the direct force measurement method needs to be combined with other strategies. 15,16 Armstrong et al 16 combined the use of a PSD with the use of position-sensitive masked detection (PSMD), selectively reflecting light in different directions using an appropriately defined mask (details about the mask can be found in Ref. 17), to perform full three-dimensional (3D) direct optical force measurements of trapped single Escherichia coli (E. coli) cells.…”

Section: Advances For Quantitative Force Measurements In Biological Systemsmentioning

confidence: 99%

Optical manipulation: advances for biophotonics in the 21st century

Corsetti

Dholakia

2021

J. Biomed. Opt.

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Significance: Optical trapping is a technique capable of applying minute forces that has been applied to studies spanning single molecules up to microorganisms.Aim: The goal of this perspective is to highlight some of the main advances in the last decade in this field that are pertinent for a biomedical audience.Approach: First, the direct determination of forces in optical tweezers and the combination of optical and acoustic traps, which allows studies across different length scales, are discussed. Then, a review of the progress made in the direct trapping of both single-molecules, and even single-viruses, and single cells with optical forces is outlined. Lastly, future directions for this methodology in biophotonics are discussed.Results: In the 21st century, optical manipulation has expanded its unique capabilities, enabling not only a more detailed study of single molecules and single cells but also of more complex living systems, giving us further insights into important biological activities.Conclusions: Optical forces have played a large role in the biomedical landscape leading to exceptional new biological breakthroughs. The continuous advances in the world of optical trapping will certainly lead to further exploitation, including exciting in-vivo experiments.

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Section: Advances For Quantitative Force Measurements In Biological Systemsmentioning

confidence: 99%

Optical manipulation: advances for biophotonics in the 21st century

Corsetti

Dholakia

2021

J. Biomed. Opt.

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“…Another example of the usefulness of precise force measurement is for studies of biological swimmers; for example, if we hold a swimming cell in an optical trap and gradually lower the trap power until the cell escapes, we can infer information about the swimming force from the optical force at the time the particle escaped (Nascimento et al, 2008). The range of forces OT can be used to measure is related to the range of forces that the OT can apply, i.e., OT can be used to measure piconewton and femtonewton scale forces, such as those encountered protein folding (Bustamante et al, 2020) or cell motility (Arbore et al, 2019;Armstrong et al, 2020).…”

Section: Optical Tweezersmentioning

confidence: 99%

Optical Tweezers Exploring Neuroscience

Lenton

Scott

Rubinsztein‐Dunlop

et al. 2020

Front. Bioeng. Biotechnol.

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Over the past decade, optical tweezers (OT) have been increasingly used in neuroscience for studies of molecules and neuronal dynamics, as well as for the study of model organisms as a whole. Compared to other areas of biology, it has taken much longer for OT to become an established tool in neuroscience. This is, in part, due to the complexity of the brain and the inherent difficulties in trapping individual molecules or manipulating cells located deep within biological tissue. Recent advances in OT, as well as parallel developments in imaging and adaptive optics, have significantly extended the capabilities of OT. In this review, we describe how OT became an established tool in neuroscience and we elaborate on possible future directions for the field. Rather than covering all applications of OT to neurons or related proteins and molecules, we focus our discussions on studies that provide crucial information to neuroscience, such as neuron dynamics, growth, and communication, as these studies have revealed meaningful information and provide direction for the field into the future.

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“…We show, in an experiment, that single descents can be used to calibrate the optical trap where large trap strengths restrict the performance of equilibrium measurements as they are in a regime where the signal is too close to the detection noise floor. This type of calibration is ideal for multiple and dynamic optical traps to rapidly determine the swimming forces of optically driven micromachines, active matter, and cells on the two micron size scale [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events

2021

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The trap stiffness us the key property in using optical tweezers as a force transducer. Force reconstruction via maximum-likelihood-estimator analysis (FORMA) determines the optical trap stiffness based on estimation of the particle velocity from statistical trajectories. Using a modification of this technique, we determine the trap stiffness for a two micron particle within 2 ms to a precision of ∼10% using camera measurements at 10 kfps with the contribution of pixel noise to the signal being larger the level Brownian motion. This is done by observing a particle fall into an optical trap once at a high stiffness. This type of calibration is attractive, as it avoids the use of a nanopositioning stage, which makes it ideal for systems of large numbers of particles, e.g., micro-fluidics or active matter systems.

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Swimming force and behavior of optically trapped micro-organisms

Cited by 22 publications

References 26 publications

Optical manipulation: advances for biophotonics in the 21st century

Optical manipulation: advances for biophotonics in the 21st century

Optical Tweezers Exploring Neuroscience

Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events

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