Swimming cells promote a dynamic environment within biofilms

Boles, Blaise R.; Horswill, Alexander R.

doi:10.1073/pnas.1210297109

Cited by 11 publications

(12 citation statements)

References 12 publications

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“…Of note, the diffusion of FITC-labeled Dextran (250 kDa) into a B. thuringiensis biofilm was enhanced by the presence of the intra-biofilm swimming phenotype. These results suggested that cellular motility within a biofilm should be considered, along with diffusion and advection, when describing mass transfer within biofilms of specific species (Boles and Horswill, 2012). …”

Section: Permeability Of Biofilmsmentioning

confidence: 99%

Material properties of biofilms—a review of methods for understanding permeability and mechanics

et al. 2015

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Microorganisms can form biofilms, which are multicellular communities surrounded by a hydrated extracellular matrix of polymers. Central properties of the biofilm are governed by this extracellular matrix, which provides mechanical stability to the three-dimensional biofilm structure, regulates the ability of the biofilm to adhere to surfaces, and determines the ability of the biofilm to adsorb gasses, solutes, and foreign cells. Despite their critical relevance for understanding and eliminating of biofilms, the materials properties of the extracellular matrix are understudied. Here, we offer the reader a guide to current technologies that can be utilized to specifically assess the permeability and mechanical properties of the biofilm matrix and its interacting components. In particular, we highlight technological advances in instrumentation and interactions between multiple disciplines that have broadened the spectrum of methods available to conduct these studies. We review pioneering work that furthers our understanding of the material properties of biofilms.

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Section: Permeability Of Biofilmsmentioning

confidence: 99%

Material properties of biofilms—a review of methods for understanding permeability and mechanics

et al. 2015

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“…Previously, it has been reported that extracellular saccharides may prevent the diffusion of antibiotics across the biofilm [6]. However, CSE1034 destroyed approximately 92% E. coli biofilm, because EDTA may acts as a facilitating agent and helps penetration of antibiotic to biofilms formed by E. coli.…”

Section: Antimicrobial Agent Percentage (%) Of Isolatesmentioning

confidence: 99%

“…Contrary to these, we found that 10 mM EDTA disrupts the bacterial biofilm when tested alone; the concentration of EDTA used in our study was five times lower than previous study. There are many reports that the bacterial cells are covered by exopolysaccharide matrix, and this matrix is stabilized by divalent cations [6,24,25].…”

Section: Antimicrobial Agent Percentage (%) Of Isolatesmentioning

confidence: 99%

“…It is thought that the curli causes cell to surface interactions, leading to adherence of bacterial cells to any of the solid surface, including living tissues, in dwelling medical devices, dental water unit systems [1,4,5], and turning the planktonic growth to a sessile biofilm mode of growth [1,6,7]. Once the biofilm is developed, the sessile bacterial cells start to exhibit more resistance to antibacterial agents than that of planktonic cells [2,8], leading to failure of antibacterial therapy.…”

Section: Introductionmentioning

confidence: 99%

“…The adhesion of the bacterial cell to any surface is an initial step of biofilm formation [5,6]. It is thought that the curli causes cell to surface interactions, leading to adherence of bacterial cells to any of the solid surface, including living tissues, in dwelling medical devices, dental water unit systems [1,4,5], and turning the planktonic growth to a sessile biofilm mode of growth [1,6,7].…”

Section: Introductionmentioning

confidence: 99%

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Role of CSE1034 in Escherichia coli Biofilm Destruction

Kumar¹

2013

J Microb Biochem Technol

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Present study was conducted to assess the effect of ethylenediamine tetraacetic acid (EDTA); a non antibiotic adjuvant, and CSE1034, a novel antibiotic adjuvant entity in biofilm destruction of Escherichia coli, and comparing the efficacy with other drugs. We first determined the susceptibility of six antimicrobial agents against planktonic cultures, as well as sessile cells of E. coli clinical isolates, using the Clinical and Laboratory Standards Institute (CLSI) method. Subsequently, effects of EDTA alone, and drugs on bacterial curli production, adhesion, and in-vitro biofilm destruction were studied. The percentage of biofilm persistence was determined with spectrophotometry. The structural damage of biofilms was studied by scanning electron microscope. It was found that, among the drugs used, CSE1034 was the most effective against all of the E. coli clinical isolates, with MIC and MBEC values ranging from 32-64 µg/ml and 256-512 µg/ml, respectively. Exposure of clinical isolates with EDTA alone caused inhibitions of curli formation and bacterial adhesion at 4 to 5 mM. Further, EDTA treatment of preformed biofilm caused complete biofilm destruction at 8-10 mM. Interestingly, CSE1034 due to presence of 10 mM EDTA led to enhanced antibacterial, as well as biofilm destruction activities. Results of spectrophotometric analysis and scanning electron microscopy revealed that approximately 92% biofilms were eradicated by the CSE1034, and not by comparator drugs. The results indicate that the CSE1034 appears to be most effective drug of choice for the treatment of infections caused by E. coli.

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An Ex‐Regulator's View of the Microbiology QA/QC Functions in the US Pharmaceutical Industries

Hussong

2019

Pharmaceutical Microbiological Quality Assurance and Control

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Swimming cells promote a dynamic environment within biofilms

Cited by 11 publications

References 12 publications

Material properties of biofilms—a review of methods for understanding permeability and mechanics

Material properties of biofilms—a review of methods for understanding permeability and mechanics

Role of CSE1034 in Escherichia coli Biofilm Destruction

An Ex‐Regulator's View of the Microbiology QA/QC Functions in the US Pharmaceutical Industries

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