Swelling of cell walls in mature sweet cherry fruit: factors and mechanisms

Schumann, Christine; Knoche, Moritz

doi:10.1007/s00425-020-03352-y

Cited by 18 publications

(22 citation statements)

References 29 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The swelling pressure determined in vitro using extracted cell wall material was very low across all developmental stage and also across the different cell wall fractions. This finding is consistent with earlier ones indicating that the swelling of cell walls is a physical process, normally counterbalanced by cell turgor (Grimm and Knoche 2015 ; Schumann and Knoche 2020 ). Swelling occurs when turgor is lost, regardless of whether this is the result of turgor release following imposition of a freeze/thaw cycle or by plasmolyzing epidermal cells by exposure to a hypertonic osmoticum (Schumann and Knoche 2020 ).…”

Section: Discussionsupporting

confidence: 93%

“…Cell wall swelling was determined using the procedure described in detail by Schumann and Knoche ( 2020 ). Briefly, epidermal skin sections (ES) were prepared from a fruit’s equator in the cheek region.…”

Section: Methodsmentioning

confidence: 99%

“…The swelling pressure was determined for the AIR samples for the developing sweet cherry fruit and also for the OXP, HSP, OHP, HC and CL fractions of mature fruit. The procedure described previously was used (Schumann and Knoche 2020 ). Briefly, AIR (25 mg per replicate for the developmental time course and the exocarp/mesocarp comparison, 20 mg per replicate for comparing fractions at maturity) was placed on a stainless steel frit in a custom-built pressure chamber (inner diameter 25.5 mm).…”

Section: Methodsmentioning

confidence: 99%

“…Malic acid is a common osmolyte in sweet cherries that occurs at a concentration of about 70 mM (Herrmann 2001 ). Loss of cell turgor causes swelling of both epidermal and hypodermal cell walls (Schumann and Knoche 2020 ). Together, these cell layers form the structural backbone of the sweet cherry fruit (Brüggenwirth et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Decreased deposition and increased swelling of cell walls contribute to increased cracking susceptibility of developing sweet cherry fruit

Schumann

Sitzenstock

Erz

et al. 2020

Planta

Self Cite

View full text Add to dashboard Cite

Main conclusion During fruit development, cell wall deposition rate decreases and cell wall swelling increases. The cell wall swelling pressure is very low relative to the fruit’s highly negative osmotic potential. Abstract Rain cracking of sweet cherry fruit is preceded by the swelling of the cell walls. Cell wall swelling decreases both the cell: cell adhesion and the cell wall fracture force. Rain cracking susceptibility increases during fruit development. The objectives were to relate developmental changes in cell wall swelling to compositional changes taking place in the cell wall. During fruit development, total mass of cell wall, of pectins and of hemicelluloses increases, but total mass of cellulose remains constant. The mass of these cell wall fractions increases at a lower rate than the fruit fresh mass—particularly during stage II and early stage III. During stage III, on a whole-fruit basis, the HCl-soluble pectin fraction, followed by the water-soluble pectin fraction, the NaOH-soluble pectin fraction and the oxalate-soluble pectin fraction all increase. At maturity, just the HCl-soluble pectin decreases. Cell wall swelling increases during stages I and II of fruit development, with little change thereafter. This was indexed by light microscopy of skin sections following turgor release, and by determinations of the swelling capacity, water holding capacity and water retention capacity. The increase in cell wall swelling during development was due primarily to increases in NaOH-soluble pectins. The in vitro swelling of cell wall extracts depends on the applied pressure. The swelling pressure of the alcohol-insoluble residue is low throughout development and surprisingly similar across different cell wall fractions. Thus, swelling pressure does not contribute significantly to fruit water potential.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decreased deposition and increased swelling of cell walls contribute to increased cracking susceptibility of developing sweet cherry fruit

Schumann

Sitzenstock

Erz

et al. 2020

Planta

Self Cite

View full text Add to dashboard Cite

show abstract

“…6). The exclusive identi cation of PIP2 proteins in in orescences (PIP2;1, PIP2;2, PIP2;5, and PIP2;7) could be related to the elevated demand for water in cauli ower in orescences, to maintain nutrient uptake and turgor [47]. The localization of PIP2 in plasma membrane samples from in orescences (Fig.…”

Section: Discussionmentioning

confidence: 96%

Plasma Membrane Vesicles From Cauliflower Meristematic Tissue And Their Role In Water Passage

García-Ibáñez

Nicolas‐Espinosa

Carvajal³

2020

Preprint

View full text Add to dashboard Cite

Background Cauliflower (Brassica oleracea L. var. botrytis) inflorescences are composed mainly of meristematic tissue, which has a high cellular proliferation. This considerable cellular density makes the inflorescence an organ with a large proportion of membranes. However, little is known about the specific role of the lipid and protein composition of the plasma membrane present in this organ. Results In this work, we analyzed the lipids and proteins present in plasma membrane from two different stages of development of cauliflower inflorescence and compared them with leaf plasma membrane. For this purpose, plasma membrane vesicles were obtained by centrifugation for each sample and the vesicular diameter and osmotic permeability (Pf) were analyzed by dynamic light scattering and the stopped-flow technique, respectively. In addition, fatty acids and sterols were analyzed by gas chromatography and HPLC, respectively. The protein composition of the inflorescences and leaves was characterized by HPLC-ESI-QTOF-MS and the data obtained were compared with Brassicaceae proteins present in the UniProt database in relation to the presence of aquaporins determined by western blot analysis. The highest Pf value was found in 90 day inflorescences-derived plasma membrane vesicles (61.4 ± 4.14 µms− 1). For sterols and fatty acids, the concentrations varied according to the organ of origin. The protein profile revealed the presence of aquaporins from the PIP1 and PIP2 subfamilies in both inflorescences and leaves. Conclusion This study shows that the composition of the sterols, the degree of unsaturation of the fatty acids, and the proteins present in the membranes analyzed give them high functionality for water passage. This represents an important addition to the limited information available in this field.

show abstract

Bionic Capsule Lithium‐Ion Battery Anodes for Efficiently Inhibiting Volume Expansion

Gao,

Rao,

Wang

et al. 2024

ChemSusChem

View full text Add to dashboard Cite

Magnetite (Fe3O4) has a large theoretical reversible capacity and rich Earth abundance, making it a promising anode material for LIBs. However, it suffers from drastic volume changes during the lithiation process, which lead to poor cycle stability and low‐rate performance. Hence, there is an urgent need for a solution to address the issue of volume expansion. Taking inspiration from how glycophyte cells mitigate excessive water uptake/loss through their cell wall to preserve the structural integrity of cells, we designed Fe3O4@PMMA multi‐core capsules by microemulsion polymerization as a kind of anode materials, also proposed a new evaluation method for real‐time repair effect of the battery capacity. The Fe3O4@PMMA anode shows a high reversible specific capacity (858.0 mAh g‐1 at 0.1 C after 300 cycles) and an excellent cycle stability (450.99 mAh g‐1 at 0.5 C after 450 cycles). Furthermore, the LiNi0.8Co0.1Mn0.1O2/Fe3O4@PMMA 200 mAh pouch cells exhibit a stable capacity (200.6 mAh) and high‐capacity retention rate (95.5%) after 450 cycles at 0.5 C. Compared to the original battery, the capacity repair rate of this battery is as high as 93.4%. This kind of bionic capsules provide an innovative solution for improving the electrochemical performance of Fe3O4 anodes to promote their industrial applications.

show abstract

Swelling of cell walls in mature sweet cherry fruit: factors and mechanisms

Abstract: Main conclusion Swelling of sweet cherry cell walls is a physical process counterbalanced by turgor. Cell turgor prevents swelling in intact cells, whereas loss of turgor allows cell walls to swell.

Cited by 18 publications

References 29 publications

Decreased deposition and increased swelling of cell walls contribute to increased cracking susceptibility of developing sweet cherry fruit

Decreased deposition and increased swelling of cell walls contribute to increased cracking susceptibility of developing sweet cherry fruit

Plasma Membrane Vesicles From Cauliflower Meristematic Tissue And Their Role In Water Passage

Bionic Capsule Lithium‐Ion Battery Anodes for Efficiently Inhibiting Volume Expansion

Contact Info

Product

Resources

About