Swelling behavior of a genetically engineered silk-elastinlike protein polymer hydrogel

Dinerman, Adam A.; Cappello, Joseph; Ghandehari, Hamidreza; Hoag, Stephen W.

doi:10.1016/s0142-9612(02)00164-3

Cited by 118 publications

(111 citation statements)

References 10 publications

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“…DSC was initially used to determine the gelation kinetics, and it showed that decreasing the number of silk blocks leads to a delay in the gelation rate at room temperature. Conversely, hydration studies showed the addition of silk blocks also reduces the swelling ratio [172,173]. Encapsulation studies have shown that the presence of either charged or uncharged solutes disrupts the formation of the gel.…”

Section: Silk-elp Hybridsmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

280

231

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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Section: Silk-elp Hybridsmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

280

231

View full text Add to dashboard Cite

show abstract

“…However, the stimuli-responsiveness of the EL block has not been exploited to any great extent to date and no complex molecular designs for that block showing advanced functionality have been reported. Furthermore, many SELR designs incorporate such a high SL percentage that the contribution of the elastomeric portion to the self-assembly process is imperceptible 27,28 . However, experience with SELR designs with a lower SL content has demonstrated that, in such compositions, the EL blocks are able to maintain their characteristic thermal transition [28][29][30] .…”

Section: Introductionmentioning

confidence: 99%

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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ABSTRACT. Although significant progress has been made in the area of injectable hydrogels for biomedical applications and model cell niches, further improvements are still needed, especially in terms of mechanical performance, stability and biomimicry of the native fibrillar architecture found in the extracellular matrix (ECM). This work focuses on the design and production of a silk-elastin-based injectable multiblock co-recombinamer that spontaneously forms a stable physical nanofibrillar hydrogel under physiological conditions. That differs from previously reported silk-elastin-like polymers on a major content and predominance of the elastin-like part, as well as a more complex structure and behavior of such part of the molecule, which is aimed to obtain well defined hydrogels.Rheological and DSC experiments showed that this system displays a coordinated and concomitant dual gelation mechanism. In a first stage, a rapid, thermally driven gelation of the co-recombinamer solution takes place once the system reaches body temperature due to the thermal responsiveness of the elastinlike (EL) parts and the amphiphilic multiblock design of the co-recombinamer. A bridged micellar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 structure is the dominant microscopic feature of this stage, as demonstrated by AFM and TEM.Completion of the initial stage triggers the second, which comprises a stabilization, reinforcement, and microstructuring of the gel. FTIR analysis shows that these events involve the formation of β-sheets around the silk motifs. The emergence of such β-sheet structures leads to the spontaneous selforganization of the gel into the final fibrous structure. Despite the absence of biological cues, here we set the basis of the minimal structure that is able to display such a set of physical properties and undergo microscopic transformation from a solution to a fibrous hydrogel. The results point to the potential of this system as a basis for the development of injectable fibrillar biomaterial platforms towards a fully functional, biomimetic, artificial extracellular matrix and cell niches.

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“…14 A responsive, reversible, and injectable drug vehicle [14][15][16][17][18] is vital for the successful delivery of drugs to a target site, so noncovalent interactions of peptide nanofibers with drugs should be studied. Peptide nanofiber assemblies with charged surface are a class of attractive scaffolds for interaction with the external surfaces of protein drugs.…”

Section: Introductionmentioning

confidence: 99%