Sweeteners

Lipinski, Gert-Wolfhard von Rymon

doi:10.1002/14356007.a26_023.pub2

Cited by 3 publications

(9 citation statements)

References 60 publications

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“…Recent reports project the annual growth rate of stevia compounds to be 6.1% and 8.2%, during 2015–2024 and 2017–2024, respectively [ 16 ]. Stevia has gained industry acceptance in recent years due to its ease of cultivation in several countries across the globe [ 16 ] and its high sweetness index (160–250 times sweeter than sucrose [ 17 ]). This shows that the growth of stevia’s use as a sugar substitute, despite taste limitations of the marketed glycosides [ 18 ], was contingent on the feasibility of its large-scale manufacturing.…”

Section: Introductionmentioning

confidence: 99%

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Kelada

Tusé²,

Gleba

et al. 2021

Foods

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There are currently worldwide efforts to reduce sugar intake due to the various adverse health effects linked with the overconsumption of sugars. Artificial sweeteners have been used as an alternative to nutritive sugars in numerous applications; however, their long-term effects on human health remain controversial. This led to a shift in consumer preference towards non-caloric sweeteners from natural sources. Thaumatins are a class of intensely sweet proteins found in arils of the fruits of the West-African plant Thaumatococcus daniellii. Thaumatins’ current production method through aqueous extraction from this plant and uncertainty of the harvest from tropical rainforests limits its supply while the demand is increasing. Despite successful recombinant expression of the protein in several organisms, no large-scale bioproduction facilities exist. We present preliminary process design, process simulation, and economic analysis for a large-scale (50 metric tons/year) production of a thaumatin II variant using several different molecular farming platforms.

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Section: Introductionmentioning

confidence: 99%

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Kelada

Tusé²,

Gleba

et al. 2021

Foods

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“…Recent reports project the annual growth rate of stevia compounds to be 6.1% and 8.2%, during 2015-2024 and 2017-2024, respectively (Ciriminna, Meneguzzo, Pecoraino, & Pagliaro, 2019). Stevia has gained industry acceptance in recent years due to its ease of cultivation in several countries across the globe (Ciriminna et al, 2019) and its high sweetness index (160-250 times sweeter than sucrose (von Rymon Lipinski, 2015). This shows that the growth of stevia's use as a sugar substitute, despite taste limitations of the marketed glycosides (Tao & Cho, 2020), was contingent on the feasibility of its large-scale manufacturing.…”

Section: Introductionmentioning

confidence: 99%

“…This work focuses on thaumatins, a class of intensely sweet proteins isolated from the arils of the fruits of the West-African plant Thaumatococcus danielli (Masuda, 2017). The distinctiveness of thaumatin lies in its sweetness index being up to 3,500 times sweeter than sugar (von Rymon Lipinski, 2015). According to 2008 Guinness World Records, it is the sweetest natural substance known to mankind (Glenday, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In 2020, the FDA granted GRAS status to recombinant thaumatin II produced in Nicotiana plants for use as a sweetener (FDA GRAS GRN 910) and as a flavor enhancer/modifier (FDA GRAS GRN 920). In the EU, thaumatins are allowed as both sweeteners and flavor enhancers (E 957) (Lee, 2000;von Rymon Lipinski, 2015). Thaumatin's safety has been extensively documented.…”

Section: Introductionmentioning

confidence: 99%

“…Notably, there have been many attempts to produce thaumatin by means of genetically engineered microorganisms and plants. Despite successfully expressing thaumatin in yeast (Masuda et al, 2004), bacteria (Illingworth, Larson, & Hellekant, 1989), fungi (Faus et al, 1998), and transgenic and transfected plants (Bartoszewski, Niedziela, Szwacka, & Niemirowicz-Szczytt, 2003;Hahn et al, 2015), biotechnological large-scale production facilities have yet to be established (von Rymon Lipinski, 2015;Joseph et al, 2019). Molecular farming, the production of recombinant proteins in plants, offers several advantages over bioreactor-based systems.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Kelada

Tusé²,

Gleba

et al. 2020

Preprint

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There are currently worldwide efforts to reduce sugar intake due to the various adverse health effects linked with the overconsumption of sugars. Artificial sweeteners have been used as an alternative to nutritive sugars in numerous applications; however, their long-term effects on human health remain controversial. This led to a shift in consumer preference towards non-caloric sweeteners from natural sources. Thaumatins are a class of intensely sweet proteins found in arils of the fruits of the West-African plant Thaumatococcus danielli. Thaumatins’ current production method through aqueous extraction from this plant and uncertainty of the harvest from tropical rainforests limits its supply while the demand is increasing. Despite successful recombinant expression of the protein in several organisms, no large-scale bioproduction facilities exist. We present preliminary process design, process simulation, and economic analysis for a large-scale (50 metric tons/year) production of thaumatin II variant by several different molecular farming platforms.

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p-Toluenesulfonic Acid-Based Deep-Eutectic Solvents for Solubilizing Metal Oxides

Rodriguez

Machiels

Binnemans

2019

ACS Sustainable Chem. Eng.

124

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Combinations of p-toluenesulfonic acid monohydrate with different hydrogen-bond acceptors (i.e., choline chloride, tetraethylammonium chloride, tetrabutylammonium chloride, betaine hydrochloride, β-alanine, tetrabutylphosphonium chloride, ethylammonium chloride, methyltriphenylphosphonium bromide, and benzyltriphenylphosphonium chloride) were tested for deep-eutectic solvent (DES) formation. Only p-toluenesulfonic acid monohydrate:choline chloride, p-toluenesulfonic acid monohydrate:tetrabutylammonium chloride, and p-toluenesulfonic acid monohydrate:tetrabutylphosphonium chloride at a 1:1 molar ratio remained liquid at room temperature. The obtained deep-eutectic solvents were characterized by determining their density and viscosity as function of temperature, and their thermal operational window (i.e., decomposition temperature and melting point/glass transition temperature). Moreover, IR spectroscopy was used to elucidate the formation of hydrogen bonds. The solubility of different metal oxides in the DES p-toluenesulfonic acid monohydrate:choline chloride was experimentally determined. The effect of the hydrogen-bond donor:hydrogen-bond acceptor molar ratio (2:1, 1:1, and 1:2) on the metal oxide solubility was explored. A comparison was made with metal oxide solubilities in other choline chloride-based DESs and in acidic aqueous solutions.

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Sweeteners

Cited by 3 publications

References 60 publications

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

p-Toluenesulfonic Acid-Based Deep-Eutectic Solvents for Solubilizing Metal Oxides

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