SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis <i>sweet13;14</i>

Isoda, Reika; Palmai, Zoltan; Yoshinari, Akira; Chen, Li-Qing; Tama, Florence; Frommer, Wolf B.; Nakamura, Masayoshi

doi:10.1101/2022.05.05.490848

Cited by 3 publications

(4 citation statements)

References 44 publications

(98 reference statements)

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“…Genetic complementation of atsweet13;14 knockout mutant with AtSWEET9, which transports sucrose but not GA, fully restored the normal pollen viability and germination phenotypes, suggesting that sucrose limitation was responsible for defective pollens (Wang et al, 2022a). These findings are validated by another study, in which the impaired pollen viability and germination phenotypes of atsweet13;14 double-knockout mutants were rescued by the sucrose-selective AtSWEET13 structural mutant (that transported only sucrose) but not by the GA-selective AtSWEET13 mutant (high-capacity GA transporter) (Isoda et al, 2022).…”

Section: Sweets In Male and Female Reproductive Organssupporting

confidence: 52%

Physiological implications of SWEETs in plants and their potential applications in improving source–sink relationships for enhanced yield

Singh

Das

Gupta

et al. 2023

Plant Biotechnology Journal

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The sugars will eventually be exported transporters (SWEET) family of transporters in plants is identified as a novel class of sugar carriers capable of transporting sugars, sugar alcohols and hormones. Functioning in intercellular sugar transport, SWEETs influence a wide range of physiologically important processes. SWEETs regulate the development of sink organs by providing nutritional support from source leaves, responses to abiotic stresses by maintaining intracellular sugar concentrations, and host-pathogen interactions through the modulation of apoplastic sugar levels. Many bacterial and fungal pathogens activate the expression of SWEET genes in species such as rice and Arabidopsis to gain access to the nutrients that support virulence. The genetic manipulation of SWEETs has led to the generation of bacterial blight (BB)resistant rice varieties. Similarly, while the overexpression of the SWEETs involved in sucrose export from leaves and pathogenesis led to growth retardation and yield penalties, plants overexpressing SWEETs show improved disease resistance. Such findings demonstrate the complex functions of SWEETs in growth and stress tolerance. Here, we review the importance of SWEETs in plant-pathogen and source-sink interactions and abiotic stress resistance. We highlight the possible applications of SWEETs in crop improvement programmes aimed at improving sink and source strengths important for enhancing the sustainability of yield. We discuss how the adverse effects of the overexpression of SWEETs on plant growth may be overcome.

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Section: Sweets In Male and Female Reproductive Organssupporting

confidence: 52%

Physiological implications of SWEETs in plants and their potential applications in improving source–sink relationships for enhanced yield

Singh

Das

Gupta

et al. 2023

Plant Biotechnology Journal

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“…Although we could not exclude the possibility that the failure of complementing atsweet13;14 by AtNPF3.1 was a result of the mechanistic differences in transporting substrates between the two transporter families, the results from SWEET9 complementation support that sucrose, not GA, is critical in rescuing the atsweet13;14 defective phenotypes of pollen viability and pollen germination. Consistent with our findings, one parallel study shows that the AtSWEET13 mutant that prefers sucrose selection was able to fully rescue the pollen viability and germination phenotype of atsweet13;14 but AtSWEET13 mutant that prefers GA selection was not (Isoda et al ., 2022 ). Moreover, the soluble sugar content (including sucrose, glucose and fructose) was dramatically reduced in pollen of atsweet13;14 , and starch was substantially accumulated in the anther wall anther of atsweet13;14 compared with those in Col‐0 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…New Phytologist (2022) 236: 525-537 www.newphytologist.com was able to fully rescue the pollen viability and germination phenotype of atsweet13;14 but AtSWEET13 mutant that prefers GA selection was not (Isoda et al, 2022). Moreover, the soluble sugar content (including sucrose, glucose and fructose) was dramatically reduced in pollen of atsweet13;14, and starch was substantially accumulated in the anther wall anther of atsweet13;14 compared with those in Col-0 (Fig.…”

Section: New Phytologistmentioning

confidence: 94%

Sucrose rather than GA transported by AtSWEET13 and AtSWEET14 supports pollen fitness at late anther development stages

et al. 2022

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Both sugar and the hormone gibberellin (GA) are essential for anther-enclosed pollen development and thus for plant productivity in flowering plants. Arabidopsis (Arabidopsis thaliana) AtSWEET13 and AtSWEET14, which are expressed in anthers and associated with seed yield, transport both sucrose and GA. However, it is still unclear which substrate transported by them directly affects anther development and seed yield.Histochemical staining, cross-sectioning and microscopy imaging techniques were used to investigate and interpret the phenotypes of the atsweet13;14 double mutant during anther development. Genetic complementation of atsweet13;14 using AtSWEET9, which transports sucrose but not GA, and the GA transporter AtNPF3.1, respectively, was conducted to test the substrate preference relevant to the biological process.The loss of both AtSWEET13 and AtSWEET14 resulted in reduced pollen viability and therefore decreased pollen germination. AtSWEET9 fully rescued the defects in pollen viability and germination of atsweet13;14, whereas AtNPF3.1 failed to do so, indicating that AtSWEET13/ 14-mediated sucrose rather than GA is essential for pollen fertility.AtSWEET13 and AtSWEET14 function mainly at the anther wall during late anther development stages, and they probably are responsible for sucrose efflux into locules to support pollen development to maturation, which is vital for subsequent pollen viability and germination.

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“…In grapes, VvSWEET7 can transport glucose and sucrose, which is involved in sugar partitioning during different fruit developmental stages (Breia et al, 2020). Similar transport activity is shown by AtSWEET13 (Han et al, 2017), which is expressed during anther development (Isoda et al, 2022). Consistently, pineapple SWEET4/11/13/18 enabled yeast mutant EBY.VW4000 to survive on a medium with either sucrose (AcSWEET18) or glucose (AcSWEET4), or both (AcSWEET11/13), indicating a broad specificity for pineapple SWEETs (Figure 11).…”

Section: Figurementioning

confidence: 81%

Identification and expression analysis of pineapple sugar transporters reveal their role in the development and environmental response

Fakher

Jakada²,

Greaves³

et al. 2022

Front. Plant Sci.

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In plants, sugars are required for several essential functions, including growth, storage, signaling, defense and reproduction. Sugar transporters carry out the controlled movement of sugars from source (leaves) to sink (fruits and roots) tissues and determine the overall development of the plant. Various types of sugar transporter families have been described in plants, including sucrose transporters (SUC/SUT), monosaccharide transporter (MST) and SWEET (from “Sugar Will Eventually be Exported Transporters”). However, the information about pineapple sugar transporters is minimal. This study systematically identified and classified 45 MST and 4 SUC/SUT genes in the pineapple genome. We found that the expression patterns of sugar transporter genes have a spatiotemporal expression in reproductive and vegetative tissues indicating their pivotal role in reproductive growth and development. Besides, different families of sugar transporters have a diel expression pattern in photosynthetic and non-photosynthetic tissues displaying circadian rhythm associated participation of sugar transporters in the CAM pathway. Moreover, regulation of the stress-related sugar transporters during cold stress indicates their contribution to cold tolerance in pineapple. Heterologous expression (yeast complementation assays) of sugar transporters in a mutant yeast strain suggested that SUT1/2 have the ability to transport sucrose, and STP13, STP26, pGlcT-L2 and TMT4 are able to transport glucose, whereas SWEET11/13 transport both sucrose and fructose. The information provided here would help researchers further explore the underlying molecular mechanism involved in the sugar metabolism of pineapple.

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SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14

Cited by 3 publications

References 44 publications

Physiological implications of SWEETs in plants and their potential applications in improving source–sink relationships for enhanced yield

Physiological implications of SWEETs in plants and their potential applications in improving source–sink relationships for enhanced yield

Sucrose rather than GA transported by AtSWEET13 and AtSWEET14 supports pollen fitness at late anther development stages

Identification and expression analysis of pineapple sugar transporters reveal their role in the development and environmental response

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