Sweet Sorghum as a Biofuel Crop

Stevens, Gene; Holou, Roland A. Y.

doi:10.1039/9781849732048-00056

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…Sweet sorghum juice is extracted from stalks using roller mills, and hydraulic and screw presses, which result in over 95% recovery of the fermentable sugars 23,24 . Extractable juice yield, reaching up to 78% of the total biomass, is one of the primary factors in obtaining a higher bioethanol yield 21…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sweet sorghum genotypes for bioethanol yield and related traits

Guden

Erdurmuş²,

Erdal³

et al. 2020

Biofuels Bioprod Bioref

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The cultivation of sweet sorghum (Sorghum bicolor (L.) Moench) for bioethanol production is receiving global attention due to the plant's sugar‐rich stalk juice, which can be fermented directly to ethanol. One of the important steps toward the development of a superior sweet sorghum cultivar is proper assessment of bioethanol‐related traits in different environments. This study was conducted to characterize global sweet sorghum accessions for bioethanol yield and related traits. To do this and to obtain a final cultivar suitable for efficient bioethanol production, two different environments were chosen to select for the characteristics studied. In total, 53 genotypes, which were selected from 551 accessions and nine cultivars, were evaluated in two contrasting environments and two consecutive years (2014–2015). The results revealed wide variability among the genotypes for the traits investigated. In all the growing years and locations, the highest values for the extractable stalk juice yield, bioethanol yield, and juice Brix content were recorded for BSS55 (22 740 L ha−1), BSS46 (1569.6 L ha−1), and BSS67 (15.63 °Bx), respectively. The genotypes that originated from the USA showed some of the highest values for bioethanol yield and related traits. Positive and very high correlations were observed between the bioethanol yield and extractable stalk juice yield (r = 0.861**) and fructose and glucose concentration (r = 0.856**). The knowledge obtained in this investigation about the variation in the bioethanol yield and related traits led to potential genotypes that can be used in sweet sorghum breeding programs for bioenergy production. © 2020 Society of Industrial Chemistry and John Wiley & Sons Ltd

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Section: Introductionmentioning

confidence: 99%

Evaluation of sweet sorghum genotypes for bioethanol yield and related traits

Guden

Erdurmuş²,

Erdal³

et al. 2020

Biofuels Bioprod Bioref

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“…Among sugar crops, sugarcane accounts for almost 80% of global sugar production. Sweet sorghum has displayed huge potential to be multiple sources of energy, food and animal feed and could be a substitute for sugarcane to produce biofuel [ 13 , 14 ]. It grows quickly in adverse stress conditions of marginal lands in tropical, subtropical and temperate zones.…”

Section: Introductionmentioning

confidence: 99%

Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum

Liu

Zhang

Gong

et al. 2021

Biotechnol Biofuels

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Background Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, shown great potential for biofuel, has lower sugar content than sugarcane. To enhance sugar content, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targetted signal peptide, was transformed into the sorghum inbred line (T×430). Results The study demonstrated that transgenic lines of grain sorghum, containing 50–60% isomaltulose, accumulated up to eightfold (1000 mM) more total sugar than the control T×430 did (118 mM) in stalks of T0 generation. Subsequently, the elite engineered lines (A5, and LSG9) were crossed with sweet sorghum (Rio, and R9188). Total sugar contents (over 750 mM), were notably higher in F1, and F2 progenies than the control Rio (480 mM). The sugar contents of the engineered lines (over 750 mM), including T0, T1, F1, and F2, are surprisingly higher than that of the field-grown sugarcane (normal range 600–700 mmol/L). Additionally, analysis of physiological characterization demonstrated that the superior progenies had notably higher rates of photosynthesis, sucrose transportation, and sink strength than the controls. Conclusions The genetic engineering approach has dramatically enhanced total sugar content in grain sorghum (T0, and T1) and hybrid sorghum (F1, and F2), demonstrating that sorghum can accumulate as high or higher sugar content than sugarcane. This research illustrates that the SI gene has enormous potential on improvement of sugar content in sorghum, particularly in hybirds and sweet sorghum. The substantial increase on sugar content would lead to significant financial benefits for industrial utilization. This study could have a substantial impact on renewable bioenergy. More importantly, our results demonstrated that the phenotype of high sugar content is inheritable and shed light on improvement for other sugar crops.

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“…Among sugar crops, sugarcane accounts for almost 80% of global sugar production. Sweet sorghum has demonstrated the huge potential to be multiple sources of energy, food and animal feed and could be a substitute for sugarcane to produce biofuel [13,14]. It grows quickly in adverse stress conditions of marginal lands in tropical, subtropical and temperate zones.…”

Section: Introductionmentioning

confidence: 99%

Stem Vacuole-targeted Sucrose Isomerase Enhances Sugar Accumulation in Sorghum

Liu

Zhang

Gong

et al. 2020

Preprint

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Background: Sugar accumulation is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high biomass, has shown great potential for biofuel. In this study, sorghum was investigated as a C4 diploid model for crops with more complicated genomes such as maize and sugarcane. To enhance sugar accumulation, the sucrose isomerase (SI) gene, driven by stem-specific promoters (A2 or LSG) with a vacuole-targeted signal peptide, was transformed into the sorghum inbred line (Tx430).Results: The study demonstrated that transgenic lines of grain sorghum, containing 50-60% isomaltulose, accumulated sevenfold (804 mM) more total sugar than the control Tx430 did (118 mM) in stalks. Subsequently, the elite engineered lines (A5, and LSG9) were crossed with sweet sorghum (R9188, and Rio). Total sugar contents (over 750 mM), were significantly higher in F1, and F2 progenies than the control Rio (480 mM). The sugar contents of the engineered lines (over 750 mM), including T0, T1, F1, and F2, are higher than that of the field-grown sugarcane (normal range 600-700 mmol/L). Additionally, physiological characterization demonstrated that the superior progenies had notably higher rates of photosynthesis, sucrose transport, and sink strength than the controls.Conclusions: The genetic engineering approach has significantly enhanced total sugar content in grain sorghum (T0, and T1) and hybrid sorghum (F1, and F2), demonstrating that sorghum can accumulate sugar contents as high or higher than sugarcane. This research puts sorghum in the spotlight and frontier as a biofuel crop, particularly as it is a shorter duration crop. The substantial increase in sugar content would lead to enormous financial benefits for industrial utilization. This study could have a substantial impact on renewable bioenergy. More importantly, our results demonstrated that the phenotype of high sugar accumulation is inheritable and shed light on improvement for other sugar crops.

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Sweet Sorghum as a Biofuel Crop

Cited by 8 publications

References 9 publications

Evaluation of sweet sorghum genotypes for bioethanol yield and related traits

Evaluation of sweet sorghum genotypes for bioethanol yield and related traits

Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum

Stem Vacuole-targeted Sucrose Isomerase Enhances Sugar Accumulation in Sorghum

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