Sweating disorders in mice with and without nerve lesions

Abstract

Background Hypersensitivity and altered sweating are often present in neuropathy patients. Nerve lesions are known to produce sudomotor dysfunctions but also patients suffering from complex regional pain syndrome, CRPS1—a condition without a nerve lesion—present with sweating disorders. Methods Using proton nuclear magnetic resonance of sweat water, we quantified sweat output of mice suffering from a nerve lesion or a bone fracture without nerve lesion and correlated their sweating with behavioural paw hypersensitivity accessed in von Frey testings, water applications and weight‐bearing measured with an incapacitance metre. Results Lesioned animals sweat less and are hypersensitive compared to healthy controls, as expected. Fractured animals on the injured side sweat less acutely after the injury but more in the chronic phase. They are hypersensitive acutely as well as chronically after the fracture. These findings resemble human bone trauma patients in the acute phase and CRPS patients in the chronic phase. Conclusions Sweating disorders are present both in neuropathic animals and in those with a bone fracture without nerve lesions, and autonomic dysfunctions might be considered as an important component in the aetiology of neuropathies. Significance Sweat output changes in mice after bone trauma, potentially indicative of posttraumatic processes leading to CRPS in humans.

Pharmacological Ischemic Conditioning with Roxadustat Does Not Affect Pain-Like Behaviors but Mitigates Sudomotor Impairment in a Murine Model of Deep Hind Paw Incision

“…Nevertheless, impeded local sweating has also been observed in mouse models of nerve injury and limb fracture, in which the association with post-traumatic processes leading to CRPS was implied. 32 Unexpectedly, our 3D immunohistochemistry data revealed a protective effect of PIC against this sudomotor dysfunction by facilitating sympathetic innervation to the eccrine glands around the wound.…”