Swarming Motility Without Flagellar Motor Switching by Reversal of Swimming Direction in E. coli

Wu, Zhengyu; He, Rui; Zhang, Rongjing; Yuan, Junhua

doi:10.3389/fmicb.2020.01042

Cited by 10 publications

(4 citation statements)

References 55 publications

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“…The surface motility of the bacteria was assessed using LB agar treated with glabridin (0.5 mg/mL) following the previously described method with minor modifications. 13 Two strains, namely BM-3994 and BM-4060, exhibiting relatively higher swarming motility, were cultivated until reaching the early stationary phase. A volume of 1 μL of the bacterial suspension was introduced onto the motility plate, followed by incubation at 37°C for 24 h, after which the surface growth zone was measured.…”

Section: Methodsmentioning

confidence: 99%

Glabridin Functions as a Quorum Sensing Inhibitor to Inhibit Biofilm Formation and Swarming Motility of Multidrug-Resistant Acinetobacter baumannii

Lin,

Zhou,

et al. 2023

IDR

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Objective Acinetobacter baumannii is a hazardous bacterium that causes hospital-acquired nosocomial infections, and the advent of multidrug-resistant A. baumannii (MDR-AB) strains is concerning. Novel antibacterial therapeutic strategies must be developed. The biological effects of glabridin on MDR-AB were investigated in this study. Methods The minimum inhibitory concentrations (MICs) of glabridin against eight clinical MDR-AB strains were determined using the broth microdilution technique. Crystal violet staining was used to assess biofilm development, which has significant contribution to bacterial resistance. Swarming motility was measured according to surface growth zone of MDR-AB on LB agar medium. qRT-PCR was used to evaluate the expression of quorum sensing genes abaI and abaR . Glabridin and routinely used therapeutic antimicrobial agents were tested for synergistic action using the checkerboard method. Results According to our findings, glabridin suppressed MDR-AB growth at high doses (512–1024 μg/mL). The 1/4 MIC of glabridin significantly decreased MDR-AB biofilm formation by 19.98% ( P < 0.05), inhibited MDR-AB motility by 44.27% ( P < 0.05), whereas the 1/2 MIC of glabridin dramatically reduced MDR-AB biofilm development by 27.43% ( P < 0.01), suppressed MDR-AB motility by 50.64% ( P < 0.05). Mechanistically, glabridin substantially downregulated the expression of quorum sensing-related genes abaI and abaR by up to 39.12% (P < 0.001) and 25.19% (P < 0.01), respectively. However, no synergistic effect between glabridin and antibacterial drugs was found. Conclusion Glabridin might be a quorum sensing inhibitor that inhibits MDR-AB biofilm development and swarming motility.

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Section: Methodsmentioning

confidence: 99%

Glabridin Functions as a Quorum Sensing Inhibitor to Inhibit Biofilm Formation and Swarming Motility of Multidrug-Resistant Acinetobacter baumannii

Lin,

Zhou,

et al. 2023

IDR

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“…The culture was then washed once with modified chemotaxis buffer [10 mM phosphate buffer (pH 7.0)], and 5 μl of the culture was placed on top of 13 g/l Nutrient broth (Fisher Scientific™) solidified with 0.2, 0.3, 0.4, 0.5 0.6, or 0.7% (w/vol) of noble agar (Fisher Scientific™). A swarming time-course assay was conducted using 0.6% noble agar added to MMAB and supplemented with 0.5% Tween-20 since preliminary data indicated its addition promoted reproducible and robust swarming ( Wu et al, 2020 ). The plates were incubated at 28°C for 24–96 h, and the diameter of the expansion rings was measured.…”

Section: Methodsmentioning

confidence: 99%

Multiple CheY Proteins Control Surface-Associated Lifestyles of Azospirillum brasilense

Ganusova

Mukherjee

et al. 2021

Front. Microbiol.

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Bacterial chemotaxis is the directed movement of motile bacteria in gradients of chemoeffectors. This behavior is mediated by dedicated signal transduction pathways that couple environment sensing with changes in the direction of rotation of flagellar motors to ultimately affect the motility pattern. Azospirillum brasilense uses two distinct chemotaxis pathways, named Che1 and Che4, and four different response regulators (CheY1, CheY4, CheY6, and CheY7) to control the swimming pattern during chemotaxis. Each of the CheY homologs was shown to differentially affect the rotational bias of the polar flagellum and chemotaxis. The role, if any, of these CheY homologs in swarming, which depends on a distinct lateral flagella system or in attachment is not known. Here, we characterize CheY homologs’ roles in swimming, swarming, and attachment to abiotic and biotic (wheat roots) surfaces and biofilm formation. We show that while strains lacking CheY1 and CheY6 are still able to navigate air gradients, strains lacking CheY4 and CheY7 are chemotaxis null. Expansion of swarming colonies in the presence of gradients requires chemotaxis. The induction of swarming depends on CheY4 and CheY7, but the cells’ organization as dense clusters in productive swarms appear to depend on functional CheYs but not chemotaxis per se. Similarly, functional CheY homologs but not chemotaxis, contribute to attachment to both abiotic and root surfaces as well as to biofilm formation, although these effects are likely dependent on additional cell surface properties such as adhesiveness. Collectively, our data highlight distinct roles for multiple CheY homologs and for chemotaxis on swarming and attachment to surfaces.

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“…Many species, including E. coli, use flagella to propel themselves to move toward food or away from noxious environments. This flagellum-mediated motility is called swimming motility, and this ability is also positively related to microbe's virulence [23]. To explore whether there is any difference in swimming motility between the isolates, we performed swimming motility assay on semi-solid agar.…”

Section: E Coli Isolated From Hag Harbors More Resistant Genesmentioning

confidence: 99%

Exposure to Veterinary Antibiotics via Food Chain Disrupts Gut Microbiota and Drives Increased Escherichia coli Virulence and Drug Resistance in Young Adults

Liu

et al. 2022

Pathogens

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Exposure to veterinary antibiotics (VAs) and preferred as veterinary antibiotics (PVAs) via the food chain is unavoidable for their extensive use not only for treating bacterial infections, but also for use as growth promoters in livestock and aquaculture. One of the consequences is the disturbance of gut microbiota. However, its impact on the virulence and drug resistance of opportunistic pathogens is still unclear. In this study, a total of 26 antibiotics were detected in the urine of 300 young undergraduates in Anhui Province. We found that excessive intake of milk was positively correlated to high levels of VAs and PVAs. It led to the dysbiosis of gut microbiota characterized by high abundance of Bacteroidetes and Proteobacteria. The increase in Proteobacteria was mainly due to a single operational taxonomic unit (OTU) of Escherichia coli (E. coli). We isolated several E. coli strains from participants and compared their drug resistance and virulence using PCR assay and virulence-related assays. We observed that exposure to high levels of VAs and PVAs induced more resistant genes and drove E. coli strain to become more virulent. At last, we conducted transcriptome analysis to investigate the molecular mechanism of virulent and drug-resistant regulators in the highly virulent E. coli strain. We noted that there were multiple pathways involved in the drug resistance and virulence of the highly virulent strain. Our results demonstrated that participants with high-level VAs and PVAs exposure have a disrupted gut microbiota following the appearance of highly drug-resistant and virulent E. coli and, therefore may be at elevated risk for long-term health complications.

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Swarming Motility Without Flagellar Motor Switching by Reversal of Swimming Direction in E. coli

Cited by 10 publications

References 55 publications

Glabridin Functions as a Quorum Sensing Inhibitor to Inhibit Biofilm Formation and Swarming Motility of Multidrug-Resistant Acinetobacter baumannii

Glabridin Functions as a Quorum Sensing Inhibitor to Inhibit Biofilm Formation and Swarming Motility of Multidrug-Resistant Acinetobacter baumannii

Multiple CheY Proteins Control Surface-Associated Lifestyles of Azospirillum brasilense

Exposure to Veterinary Antibiotics via Food Chain Disrupts Gut Microbiota and Drives Increased Escherichia coli Virulence and Drug Resistance in Young Adults

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