Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. Abstract: Macroscopic traffic flow model calibration is an optimisation problem typically solved by a derivative-free population based stochastic search methods. This paper reports on the use of a gradient based algorithm using automatic differentiation. The ADOL-C library is coupled with the METANET source code and this system is embedded within an optimisation algorithm based on RPROP. The result is a very efficient system which is able to be calibrate METANET's second order model by determining the density and speed equation parameters as well as the fundamental diagrams used. Information obtained from the system's Jacobian provides extra insight into the system dynamics. A 22 km site is considered near Sheffield, UK and the results of a typical calibration and validation process are reported.