SWAPDT: A method for Short-time Withering Assessment of Probability for Drought Tolerance in Camellia sinensis validated by targeted metabolomics

Nyarukowa, Christopher; Koech, Robert; Loots, Theodor; Apostolides, Zeno

doi:10.1016/j.jplph.2016.04.004

Cited by 21 publications

(20 citation statements)

References 39 publications

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“…Furthermore, MATE proteins have facilitated and modulated abscisic acid efflux and sensitivity in drought-tolerant Arabidopsis 46 . Since a putative QTL for %RWC annotated as MATE in this current study, it therefore, corroborates with previous reports 47 which relied on the percent %RWC to classify tea cultivars as either drought tolerant or drought susceptible based on tea leaves metabolites.…”

Section: Discussionsupporting

confidence: 91%

Functional annotation of putative QTL associated with black tea quality and drought tolerance traits

Koech

Malebe

Nyarukowa

et al. 2019

Sci Rep

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The understanding of black tea quality and percent relative water content (%RWC) traits in tea (Camellia sinensis) by a quantitative trait loci (QTL) approach can be useful in elucidation and identification of candidate genes underlying the QTL which has remained to be difficult. The objective of the study was to identify putative QTL controlling black tea quality and percent relative water traits in two tea populations and their F1 progeny. A total of 1,421 DArTseq markers derived from the linkage map identified 53 DArTseq markers to be linked to black tea quality and %RWC. All 53 DArTseq markers with unique best hits were identified in the tea genome. A total of 5,592 unigenes were assigned gene ontology (GO) terms, 56% comprised biological processes, cellular component (29%) and molecular functions (15%), respectively. A total of 84 unigenes in 15 LGs were assigned to 25 different Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways based on categories of secondary metabolite biosynthesis. The three major enzymes identified were transferases (38.9%), hydrolases (29%) and oxidoreductases (18.3%). The putative candidate proteins identified were involved in flavonoid biosynthesis, alkaloid biosynthesis, ATPase family proteins related to abiotic/biotic stress response. The functional annotation of putative QTL identified in this current study will shed more light on the proteins associated with caffeine and catechins biosynthesis and % RWC. This study may help breeders in selection of parents with desirable DArTseq markers for development of new tea cultivars with desirable traits.

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Section: Discussionsupporting

confidence: 91%

Functional annotation of putative QTL associated with black tea quality and drought tolerance traits

Koech

Malebe

Nyarukowa

et al. 2019

Sci Rep

Self Cite

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“…RWC varies according to genotypes, with resistant genotypes maintaining higher RWC compared to susceptible ones (Maritim et al, 2015). Furthermore, a method for Shorttime Withering Assessment of Probability for Drought Tolerance (SWAPDT) validated by targeted metabolomics for predicting the drought tolerance (DT) in tea was developed (Nyarukowa et al, 2016). The method relies on the percent RWC of tea leaves after 5 h under withering conditions.…”

Section: Physiological Responsesmentioning

confidence: 99%

Combating Climate Change in the Kenyan Tea Industry

et al. 2020

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Climate change triggered by global warming poses a major threat to agricultural systems globally. This phenomenon is characterized by emergence of pests and diseases, extreme weather events, such as prolonged drought, high intensity rains, hailstones and frosts, which are becoming more frequent ultimately impacting negatively to agricultural production including rain-fed tea cultivation. Kenya is predominantly an agricultural based economy, with the tea sector generating about 26% of the total export earnings and about 4% gross domestic product (GDP). In the recent years, however, the country has witnessed unstable trends in tea production associated with climate driven stresses. Toward mitigation and adaptation of climate change, multiple approaches for impact assessment, intensity prediction and adaptation have been advanced in the Kenyan tea sub-sector. Further, pressure on tea breeders to release improved climate-compatible cultivars for the rapidly deteriorating environment has resulted in the adoption of a multitargeted approach seeking to understand the complex molecular regulatory networks associated with biotic and abiotic stresses adaptation and tolerance in tea. Genetic modeling, a powerful tool that assists in breeding process, has also been adopted for selection of tea cultivars for optimal performance under varying climatic conditions. A range of physiological and biochemical responses known to counteract the effects of environmental stresses in most plants that include lowering the rates of cellular growth and net photosynthesis, stomatal closure, and the accumulation of organic solutes such as sugar alcohols, or osmolytes have been used to support breeding programs through screening of new tea cultivars suitable for changing environment. This review describes simulation models combined with high resolution climate change scenarios required to quantify the relative importance of climate change on tea production. In addition, both biodiversity and ecosystem based approaches are described as a part of an overall adaptation strategy to mitigate adverse effects of climate change on tea in Kenya and gaps highlighted for urgent investigations.

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“…Free radical content, antioxidative systems, and osmo-protectant contents were enhanced. In addition, the contents of some characteristic tea plant components, such as the contents of caffeine, polyphenols, and theanine, which are components composing tea aroma, and the corresponding synthetic genes, together with the expression of many related genes, are also affected by drought stress [2][3][4][5][6][7][8] . Moreover, other plant species also show a series of complex changes related to oxidative stress and antioxidant defence to resist drought stress [9][10][11] .…”

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Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

Wang

Xie

et al. 2020

Sci Rep

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Drought stress triggers a series of physiological and biochemical changes in tea plants. It is well known that flavonoids, lignin and long-chain fatty acids play important roles in drought resistance. However, changes in proteins related to these three metabolic pathways in tea plants under drought stress have not been reported. We analysed the proteomic profiles of tea plants by tandem mass tag and liquid chromatography-tandem mass spectrometry. A total of 4789 proteins were identified, of which 11 and 100 showed up- and downregulation, respectively. The proteins related to the biosynthesis of lignin, flavonoids and long-chain fatty acids, including phenylalanine ammonia lyase, cinnamoyl-CoA reductase, peroxidase, chalcone synthase, flavanone 3-hydroxylase, flavonol synthase, acetyl-CoA carboxylase 1,3-ketoacyl-CoA synthase 6 and 3-ketoacyl-CoA reductase 1, were downregulated. However, the contents of soluble proteins, malondialdehyde, total phenols, lignin and flavonoids in the tea plants increased. These results showed that tea plants might improve drought resistance by inhibiting the accumulation of synthases related to lignin, flavonoids and long-chain fatty acids. The proteomic spectrum of tea plants provides a scientific basis for studying the pathways related to lignin, flavonoid and long-chain fatty acid metabolism in response to drought stress.

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SWAPDT: A method for Short-time Withering Assessment of Probability for Drought Tolerance in Camellia sinensis validated by targeted metabolomics

Cited by 21 publications

References 39 publications

Functional annotation of putative QTL associated with black tea quality and drought tolerance traits

Functional annotation of putative QTL associated with black tea quality and drought tolerance traits

Combating Climate Change in the Kenyan Tea Industry

Drought stress triggers proteomic changes involving lignin, flavonoids and fatty acids in tea plants

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