SVF-derived extracellular vesicles carry characteristic miRNAs in lipedema

Priglinger, Eleni; Strohmeier, Karin; Weigl, Moritz; Lindner, Carolin; Barsch, Martin; Jacak, Jaroslaw; Redl, Heinz; Grillari, Johannes; Sandhofer, Matthias; Hackl, Matthias; Wolbank, Susanne

doi:10.1101/767483

Cited by 1 publication

(1 citation statement)

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“…It has also been used to document the distinct proteomic signature in small exosomes isolated from human induced pluripotent stem cell (iPSC)-derived neurons, plasma of dilated cardiomyopathy patients, and human fetal amniotic fluid stem cells (59,65,66). Moreover, the technique has been implied in the identification of extracellular miRNAs in small exosomes isolated from saliva, a stromal vascular fraction (SVF), and from nasal lavages (67)(68)(69). The purity of the SEC is especially recognizable when compared to other isolation methods, as shown in comparative studies with PEG-based isolation and ultracentrifugation (54,70).…”

Section: Ideal Methods For Exosome Isolation: Secmentioning

confidence: 99%

A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Sidhom¹,

Patience²,

Saleem³

2020

Preprint

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Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication. EVs are classified into several subtypes based on their origin, physical characteristics, and biomolecular makeup. Exosomes, a subtype of EVs, are released by the fusion of multivesicular bodies (MVB) with the plasma membrane of the cell. Several methods have been described in literature to isolate exosomes from biofluids including blood, urine, milk, and cell culture media among others. While differential ultracentrifugation (dUC), has been widely used to isolate exosomes, other techniques including ultrafiltration, precipitating agents such as poly-ethylene glycol (PEG), immunoaffinity capture, microfluidics and size exclusion chromatography (SEC) have emerged as credible alternatives with pros and cons associated with each. In this review, we provide a summary of commonly used exosomal isolation methodologies with a focus on SEC as an ideal methodology. We argue that exosomes isolated via SEC are relatively pure and functional, and that this methodology is reproducible and scalable, of low-cost, and does not require specialized equipment or user expertise.

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