Svalbard Fjord Sediments as a Hotspot of Functional Diversity and a Reservoir of Antibiotic Resistance

Abstract

The sea bottom acts as a key natural archive where the memory of long-term timescale environmental changes is recorded. This study discusses some ecological and chemical features of fjord sediments that were explored during the AREX cruise carried out in the Svalbard archipelago in the summer of 2021. The activity rates of the enzymes leucine aminopeptidase (LAP), beta-glucosidase (GLU), and alkaline phosphatase (AP) and community-level physiological profiles (CLPPs) were studied with the aim of determining the functional diversity of the benthic microbial community, while bacterial isolates were screened for their susceptibility to antibiotics in order to explore the role of these extreme environments as potential reservoirs of antibiotic resistance. Enzyme activity rates were obtained using fluorogenic substrates, and CLPPs were obtained using Biolog Ecoplates; antibiotic susceptibility assays were performed through the standard disk diffusion method. Spatial trends observed in the functional profiles of the microbial community suggested variability in the microbial community’s composition, presumably related to the patchy distribution of organic substrates. Complex carbon sources, carbohydrates, and amino acids were the organic polymers preferentially metabolized by the microbial community. Multi-resistance to enrofloxacin and tetracycline was detected in all of the examined samples, stressing the role of sediments as a potential reservoir of chemical wastes ascribable to antibiotic residuals. This study provides new insights on the health status of fjord sediments of West Spitsbergen, applying a dual ecological and biochemical approach. Microbial communities in the fjord sediments showed globally a good functional diversity, suggesting their versatility to rapidly react to changing conditions. The lack of significant diversification among the three studied areas suggests that microbial variables alone cannot be suitable descriptors of sediment health, and that additional measures (i.e., physical–chemical characteristics) should be taken to better define environmental status.