SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes

Kawano, Masaaki; Doi, Keiko; Fukuda, Hajime; Kita, Yoshinori; Imai, Kensuke; Inoue, Takamitsu; Enomoto, Teruya; Matsui, Masanao; Hatakeyama, Mamoru; Yamaguchi, Yuki; Handa, Hiroshi

doi:10.1016/j.btre.2014.12.008

Cited by 8 publications

(13 citation statements)

References 25 publications

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“…The major advantage of an in vitro self-assembling system like SV40's is the ease of incorporating nonviral materials into the capsid as opposed to inserting material into a preassembled capsid or engineering an in vivo expression system. Different materials have been encapsulated by SV40 VLPs, including foreign DNA of up to 17.7 kbp (7,10,56). For our in vitro SV40 assembly system, we only used the major capsid protein VP1, whereas in literature, VP2 and VP3 are shown to contribute to the infectivity in vivo (29,(57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, a viral-based system has the advantage of having a high transfection efficiency, and SV40 has been shown to be an efficient gene delivery system (5,6). In vitro, VP1 pentamers can assemble around different materials including foreign DNA up to 17.7 kbp and several types of artificial cargo (7)(8)(9)(10)(11)(12)(13)(14). Also, the surface can be modified to create targeted VLPs (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

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Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles

Rosmalen

Zlotnick

et al. 2018

Biophysical Journal

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Simian virus 40 (SV40) is a possible vehicle for targeted drug delivery systems because of its low immunogenicity, high infectivity, and high transfection efficiency. To use SV40 for biotechnology applications, more information is needed on its assembly process to efficiently incorporate foreign materials and to tune the mechanical properties of the structure. We use atomic force microscopy to determine the effect of double-stranded DNA packaging, buffer conditions, and incubation time on the morphology and strength of virus-like particles (VLPs) composed of SV40 VP1 pentamers. DNA-induced assembly results in a homogeneous population of native-like, $45 nm VLPs. In contrast, under high-ionic-strength conditions, the VP1 pentamers do not seem to interact consistently, resulting in a heterogeneous population of empty VLPs. The stiffness of both in-vitro-assembled empty and DNA-filled VLPs is comparable. Yet, the DNA increases the VLPs' resistance to large deformation forces by acting as a scaffold, holding the VP1 pentamers together. Both disulfide bridges and Ca 2þ , important in-vitro-assembly factors, affect the mechanical stability of the VLPs: the reducing agent DTT makes the VLPs less resistant to mechanical stress and prone to damage, whereas Ca 2þ-chelating EDTA induces a marked softening of the VLP. These results show that negatively charged polymers such as DNA can be used to generate homogeneous particles, thereby optimizing VLPs as vessels for drug delivery. Moreover, the storage buffer should be chosen such that VP1 interpentamer interactions are preserved.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles

Rosmalen

Zlotnick

et al. 2018

Biophysical Journal

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“…VP 1-2-3 are structural proteins that enable viral DNA to be packaged into the SV40 virion. A total of 360 VP 1 molecules form, with 72 pentamers, the virion (22, 23). The internal face of each pentamer binds a single copy of VP 2 or VP 3 (22).…”

Section: Sv40 Genomic Organizationmentioning

confidence: 99%

Association Between Simian Virus 40 and Human Tumors

et al. 2019

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Simian virus 40 (SV40) is a small DNA tumor virus of monkey origin. This polyomavirus was administered to human populations mainly through contaminated polio vaccines, which were produced in naturally infected SV40 monkey cells. Previous molecular biology and recent immunological assays have indicated that SV40 is spreading in human populations, independently from earlier SV40-contaminated vaccines. SV40 DNA sequences have been detected at a higher prevalence in specific human cancer specimens, such as the brain and bone tumors, malignant pleural mesotheliomas, and lymphoproliferative disorders, compared to the corresponding normal tissues/specimens. However, other investigations, which reported negative data, did not confirm an association between SV40 and human tumors. To circumvent the controversies, which have arisen because of these molecular biology studies, immunological researches with newly developed indirect ELISA tests were carried out in serum samples from patients affected by the same kind of tumors as mentioned above. These innovative indirect ELISAs employ synthetic peptides as mimotopes/specific SV40 antigens. SV40 mimotopes do not cross-react with the homologous human polyomaviruses, BKPyV, and JCPyV. Immunological data obtained from indirect ELISAs, using SV40 mimotopes, employed to analyze serum samples from oncological patients, have indicated that these sera had a higher prevalence of antibodies against SV40 compared to healthy subjects. The main data on (i) the biology and genetics of SV40; (ii) the epidemiology of SV40 in the general population, (iii) the mechanisms of SV40 transformation; (iv) the putative role of SV40 in the onset/progression of specific human tumors, and (v) its association with other human diseases are reported in this review.

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“…Scale bar = 100 and 25 nm (insets). Reprinted with permission from Kawano et al ( 2015 ). (C) (i) A 3D reconstruction of a confocal fluorescence microscope image of perfluorodecalin droplets coated with CPMV/biotin after being cross-linked with avidin for 3 h at 48°C.…”

Section: Viruses and Virus-like Particlesmentioning

confidence: 99%

Particle-Stabilized Fluid-Fluid Interfaces: The Impact of Core Composition on Interfacial Structure

2018

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The encapsulation of small molecule drugs in nanomaterials has become an increasingly popular approach to the delivery of therapeutics. The use of emulsions as templates for the synthesis of drug impregnated nanomaterials is an exciting area of research, and a great deal of progress has been made in understanding the interfacial chemistry that is critical to controlling the physicochemical properties of both the encapsulated material and the templated material. For example, control of the interfacial tension between an oil and aqueous phase is a fundamental concern when designing drug delivery vehicles that are stabilized by particulate surfactants at the fluid interface. Particles in general are capable of self-assembly at a fluid interface, with a preference for one or the other of the phases, and much work has focussed on modification of the particle properties to optimize formation and stability of the emulsion. An issue arises however when a model, single oil system is translated into more complex, real-world scenarios, which are often multi-component, with the incorporation of charged active ingredients and other excipients. The result is potentially a huge change in the properties of the dispersed phase which can lead to a failure in the capability of particles to continue to stabilize the interface. In this mini-review, we will focus on two encapsulation strategies based on the selective deposition of particles or proteins on a fluid-fluid interface: virus-like particles and polymer microcapsules formed from particle-stabilized emulsion templates. The similarity between these colloidal systems lies in the fact that particulate entities are used to stabilize fluid cores. We will focus on those studies that have described the effect of subtle changes in core composition on the self-assembly of particles at the fluid-fluid interface and how this influences the resulting capsule structure.

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SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beads in vitro regardless of their sizes and shapes

Cited by 8 publications

References 25 publications

Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles

Effect of dsDNA on the Assembly Pathway and Mechanical Strength of SV40 VP1 Virus-like Particles

Association Between Simian Virus 40 and Human Tumors

Particle-Stabilized Fluid-Fluid Interfaces: The Impact of Core Composition on Interfacial Structure

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