Sustained upregulation in embryonic spinal neurons of a Kv3.1 potassium channel gene encoding a delayed rectifier current

Gurantz, Devorah; Lautermilch, Nathan J.; Watt, Stephen D.; Spitzer, Nicholas C.

doi:10.1002/(sici)1097-4695(20000215)42:3<347::aid-neu6>3.0.co;2-j

Cited by 28 publications

(10 citation statements)

References 33 publications

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“…When expressed heterologously, Kv2.2 mRNA alone generates currents typical of type I neurons (Burger and Ribera, 1996), a result that does not support Kv2.2 as a molecular determinant of Kv current in type II neurons. Similar results have been obtained for the other Kv genes expressed in the embryonic Xenopus spinal cord (Ribera and Nguyen, 1993;Lazaroff et al, 1999;Gurantz et al, 2000;Fry et al, 2004 (Fantozzi et al, 2006). Changes in neuronal activity have been shown to induce homeostatic changes allowing neurons to respond appropriately (Turrigiano et al, 1994;Golowasch et al, 1999;Turrigiano, 1999;Davis and Bezprozvanny, 2001).…”

Section: Discussionsupporting

confidence: 76%

“…Kv␤4 is detected diffusely, mostly in intermediate regions. Expression of Kv1.1, Kv2.2, and Kv3.1 genes has also been examined for neurons in vitro and found to temporally parallel the patterns observed in vivo (Gurantz et al, 1996(Gurantz et al, , 2000. These results indicate that the mechanisms that direct expression of potassium currents of type I and II neurons occur either by the time of neural plate formation (St 15, the time of culture) or independently of cell-cell interactions.…”

Section: Discussionmentioning

confidence: 79%

“…Kv2.2 mRNA predominates in ventral regions, the location of VN type II neurons (Burger and Ribera, 1996). Kv3.1 transcripts appear in a more intermediate region that resembles the expression domain of glutamic acid decarboxylase mRNA, a marker for GABAergic interneurons (Gurantz et al, 2000;Watt et al, 2000). Kv␤4 is detected diffusely, mostly in intermediate regions.…”

Section: Discussionmentioning

confidence: 99%

“…Kv genes expressed in the embryonic Xenopus spinal cord include Kv1.1, Kv1.2, Kv1.4, Kv1.10, Kv2.2, Kv3.1, Kv␤4, and Kv␤2 (Ribera and Nguyen, 1993;Burger and Ribera, 1996;Lazaroff et al, 1999;Gurantz et al, 2000;Fry et al, 2001). Interestingly, the expression patterns of the majority of these genes show regional differences along the dorsal-ventral axis.…”

Section: Discussionmentioning

confidence: 99%

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Dorsal–Ventral Gradient for Neuronal Plasticity in the Embryonic Spinal Cord

Pineda

Ribera²

2008

J. Neurosci.

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Within the developing Xenopus spinal cord, voltage-gated potassium (Kv) channel genes display different expression patterns, many of which occur in opposing dorsal-ventral gradients. Regional differences in Kv gene expression would predict different patterns of potassium current (I Kv ) regulation. However, during the first 24 h of postmitotic differentiation, all primary spinal neurons undergo a temporally coordinated upregulation of I Kv density that shortens the duration of the action potential. Here, we tested whether spinal neurons demonstrate regional differences in I Kv regulation subsequent to action potential maturation. We show that two types of neurons, I and II, can be identified in culture on the basis of biophysical and pharmacological properties of I Kv and different firing patterns. Chronic increases in extracellular potassium, a signature of high neuronal activity, do not alter excitability properties of either neuron type. However, elevating extracellular potassium acutely after the period of action potential maturation leads to different changes in membrane properties of the two types of neurons. I Kv of type I neurons gains sensitivity to the blocker XE991, whereas type II neurons increase I Kv density and fire fewer action potentials. Moreover, by recording from neurons in vivo, we found that primary spinal neurons can be identified as either type I or type II. Type I neurons predominate in dorsal regions, whereas type II neurons localize to ventral regions. The findings reveal a dorsal-ventral gradient for I Kv regulation and a novel form of neuronal plasticity in spinal cord neurons.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Dorsal–Ventral Gradient for Neuronal Plasticity in the Embryonic Spinal Cord

Pineda

Ribera²

2008

J. Neurosci.

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“…A delayed rectifier, once activated, it rapidly repolarizes the cell membrane during an action potential so as to shorten its duration. This property ensures the ability for high frequency firing of the neuron (Reyes et al, 1994;Wang et al, 1998a;Gan and Kaczmarek, 1998;Rudy et al, 1999;Gurantz et al, 2000;Henne and Jeserich, 2004;Rudy and McBain, 2001;Macia et al, 2003). Kv3.1 is abundantly expressed in auditory neurons involved in preserving interaural-time differences (ITD) (Trussell, 1999;Parameshwaran-Lyer et al, 2001.…”

Section: Introductionmentioning

confidence: 99%

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Feng

Morest

2006

Hearing Research

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Expression of voltage‐dependent potassium channels in the developing visual system of Xenopus laevis

Pollock

Ferguson

McFarlane

2002

J of Comparative Neurology

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Accumulating evidence suggests that voltage-dependent potassium (Kv) channels have important and varied roles in the development of neuronal and non-neuronal cell types. They have been implicated in processes such as proliferation, cell adhesion, migration, neurite outgrowth, and axon guidance. In this study, we used antibodies against several electrically active Kv channel alpha-subunits (Kv1-4) to describe the spatial and temporal expression patterns of Kv channel subunits in Xenopus laevis retinal ganglion cell (RGC) somata, axons, and growth cones. We found that RGCs express Kv1.3-, Kv1.5-, Kv3.4-, and Kv4.2-like subunits. Each subunit displayed unique cellular and subcellular distributions. Moreover, the expression patterns changed considerably over the major period of Xenopus retinal cell genesis and differentiation. Weak or no immunoreactivity was observed with antibodies against Kv1.1, Kv1.2, Kv1.4, Kv1.6, and Kv3.2 subunits in RGCs or other retinal cell types. In support of our previous pharmacologic evidence implicating Kv channels in RGC axon outgrowth, we found that Kv1.5-, Kv3.4-, and Kv4.2-like proteins, but not Kv1.3-like subunits, are abundantly expressed in RGC growth cones.

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Sustained upregulation in embryonic spinal neurons of a Kv3.1 potassium channel gene encoding a delayed rectifier current

Cited by 28 publications

References 33 publications

Dorsal–Ventral Gradient for Neuronal Plasticity in the Embryonic Spinal Cord

Dorsal–Ventral Gradient for Neuronal Plasticity in the Embryonic Spinal Cord

Development of synapses and expression of a voltage-gated potassium channel in chick embryonic auditory nuclei

Expression of voltage‐dependent potassium channels in the developing visual system of Xenopus laevis

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