Sustained Transgene Expression by Transfection of Renin Gene into Liver of Neonates

Site-specific gene delivery in vivo through engineered Sendai viral envelopes

“…The liver is an ideal organ for transfection of a gene whose product is secreted into the circulation and is important for systemic gene therapy for several inherited diseases. Moreover, the nonpathogenicity of Sendai virus to humans and its little immunogenicity have together increased the prospects of F-virosomes in gene therapy (18,19). The results presented here by using two reporter genes independently are highly supportive of this notion.…”

“…Besides the known risk of toxicity of cationic lipids and the transient expression of CAT gene, the efficiency of this system is Ϸ10-12 times less (20) than that of the F-virosomal delivery. In spite of a sustained insulin gene expression through Sendai virus-liposome complex, this system lacks cell-type specificity and may have undesirable side effects because of the presence of the sialic acid-binding protein (HN) of viral origin (19). The ''Trojan Horse'' strategy of reconstituted Sendai virus envelope in the field of drug delivery and gene therapy as reviewed earlier (21) also suffers from the lack of target specificity.…”

Intrathecal injection of HVJ‐E containing HGF gene to cerebrospinal fluid can prevent and ameliorate hearing impairment in rats

“…Organs (brain, lung, spleen, liver, and cochlea) were harvested and placed individually in 50 ml FALCON tubes. Luciferase activity was measured using a luciferase assay kit (Promega), as described previously (30). Luciferase levels were normalized by determining the protein concentrations of the tissue extracts (31).…”