Sustained Tolerance to a Specific Effect of Ethanol on Posttetanic Potentiation in
            <i>Aplysia</i>

Traynor, M. Elaine; Woodson, PB; Schlapfer, W. T.; Barondes, S. H.

doi:10.1126/science.181841

Cited by 28 publications

(2 citation statements)

References 7 publications

(3 reference statements)

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“…For example, chronic exposure to EtOH has been shown to disrupt long‐term potentiation (LTP) in the hippocampus (reviewed in Zorumski et al., ) and lateral amygdala (Stephens et al., ) and has been shown to produce LTP‐like effects through presynaptic and postsynaptic mechanisms in the BLA (Christian et al., , ; Läck et al., ). Furthermore, chronic exposure has also been shown to modulate short‐term plasticity including paired‐pulse facilitation at both glutamatergic and GABAergic synapses (reviewed in McCool, ) as well as the magnitude and duration of PTP (Gage and Hubbard, ; Traynor et al., ).…”

Section: Discussionmentioning

confidence: 99%

Strain‐Dependent Effects of Acute Alcohol on Synaptic Vesicle Recycling and Post‐Tetanic Potentiation in Medial Glutamate Inputs to the Mouse Basolateral Amygdala

Gioia

McCool

2017

Alcoholism Clin & Exp Res

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Background Inbred mouse strains are differentially sensitive to the acute effects of ethanol and are useful tools for examining how unique genomes differentially affect alcohol-related behaviors and physiology. DBA/2J mice have been shown to be sensitive to the acute anxiolytic effects of alcohol as well as the anxiogenic effects of withdrawal from chronic alcohol exposure, while B6 mice are resistant to both. Considering that the basolateral amygdala is an important brain region for the acute and chronic effects of ethanol on fear and anxiety related behaviors, we hypothesized that there would be strain-dependent differences in the acute effects of ethanol in BLA slices. Methods We utilized patch clamp electrophysiology in BLA coronal slices from four inbred mouse strains (A/J, BALBcJ, C57BL/6J and DBA2/J) to examine how genetic background influences acute ethanol effects on synaptic vesicle recycling and post-tetanic potentiation in response to low (2 Hz) and high (40 Hz) frequency stimulation. Results We found that ethanol inhibited synaptic vesicle recycling in a strain- and stimulation frequency-dependent manner. Vesicle recycling in DBA/2J and BALBcJ cells was inhibited by acute ethanol during both low and high frequency stimulation while recycling measured from AJ cells was sensitive only during high frequency stimulation. Recycling at C57BL/6J synapses was insensitive to ethanol regardless of stimulation frequency. We additionally found that cells from DBA/2J and BALBcJ mice were sensitive to ethanol-mediated inhibition of post-tetanic potentiation. Conclusions Acute ethanol application inhibited vesicle recycling and post-tetanic potentiation at glutamatergic synapses in both a strain- and frequency-dependent fashion. Several presynaptic proteins that contribute to synaptic vesicle priming in addition to post-tetanic potentiation have been implicated in alcohol-related behaviors, including Munc13, Munc18, and RIM proteins, making them potential candidates for the molecular mechanism controlling these effects.

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Section: Discussionmentioning

confidence: 99%

Strain‐Dependent Effects of Acute Alcohol on Synaptic Vesicle Recycling and Post‐Tetanic Potentiation in Medial Glutamate Inputs to the Mouse Basolateral Amygdala

Gioia

McCool

2017

Alcoholism Clin & Exp Res

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“…There have also been reports of tolerance occurring without any signs of physical dependence. For example, Traynor, Woodson, Schlapfer, and Barondes (1976) found that several hours of intermittent ethanol exposure produced tolerance to ethanol's effect on the decay of posttetanic potentiation in the abdominal ganglion of Aplysia but that no abnormality was evident in the tissue after the cessation of ethanol exposure. In addition, Mana and Pinel (1987) reported that amygdala-kindled rats that were made tolerant to ethanol's anticonvulsant effect displayed no evidence of an increase in the duration of elicited seizures when ethanol was completely withdrawn.…”

Section: Discussionmentioning

confidence: 99%

Effect of an ascending dose regimen on the development of tolerance to the anticonvulsant effect of diazepam.

Kalynchuk¹,

Kim²,

Pinel³

et al. 1994

Behavioral Neuroscience

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The effect of an ascending dose regimen on the development of tolerance to diazepam's anticonvulsant effect was assessed. During the 22 trials of the tolerance development phase, amygdala-kindled rats received either a series of dosage injections ranging from high (10 mg/kg), to low (1.0 mg/kg), and ascending (1.0 mg/kg and increased by 0.2-mg/kg increments to 3.0 mg/kg) or saline injections. Diazepam was administered by ip injection once every 48 hr, and each injection was followed 1 hr later by a convulsive stimulation. The ascending dose rats displayed significantly more tolerance to the anticonvulsant effect of diazepam than did the high dose, low dose, or saline rats. By contrast, both the ascending and high dose rats displayed a significant withdrawal effect (i.e., increased duration of convulsions) after the cessation of diazepam injections. Results demonstrate that administration of ascending dosages can facilitate the development of tolerance to anticonvulsant drug effects and that tolerance and withdrawal are not necessarily inextricably related.

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Stimulation is necessary for the development of tolerance to a neuronal effect of ethanol

1980

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Ethanol accelerates the decay of post-tetanic potentiation at an identified synapse in Aplysia. We have previously shown that with repeated exposures the ethanol effect diminishes, a development termed "tolerance." Here we present evidence that the establishment of tolerance depends on a adequate stimulation of the presynaptic terminal in the presence of ethanol. Elevated magnesium in the perfusion medium prevents tolerance, whereas elevated calcium in the perfusion medium reduces the amount of stimulation required for tolerance to develop.

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Sustained Tolerance to a Specific Effect of Ethanol on Posttetanic Potentiation in Aplysia

Cited by 28 publications

References 7 publications

Strain‐Dependent Effects of Acute Alcohol on Synaptic Vesicle Recycling and Post‐Tetanic Potentiation in Medial Glutamate Inputs to the Mouse Basolateral Amygdala

Strain‐Dependent Effects of Acute Alcohol on Synaptic Vesicle Recycling and Post‐Tetanic Potentiation in Medial Glutamate Inputs to the Mouse Basolateral Amygdala

Effect of an ascending dose regimen on the development of tolerance to the anticonvulsant effect of diazepam.

Stimulation is necessary for the development of tolerance to a neuronal effect of ethanol

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