Sustained Release Properties of Polyelectrolyte Multilayer Capsules

Antipov, A. A.; Sukhorukov, Gleb B.; Donath, Edwin; Möhwald, Helmuth

doi:10.1021/jp002184+

Cited by 353 publications

(336 citation statements)

References 19 publications

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“…2B). This observation is in agreement with similar permeation studies based on multilayer capsules, [29,30,32,33] where a weight-average molecular weight (M w ) cutoff between 4000 and 70 000 g mol ±1 for uncharged molecules was found. [29] The fact that for the low-molecular-weight species permeation occurs within minutes, allows estimation of the diffusion constant of the molecules through the membrane to be made.…”

supporting

confidence: 91%

Filled Microcavity Arrays Produced by Polyelectrolyte Multilayer Membrane Transfer

et al. 2005

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rate was kept at 100 sccm. After the furnace had cooled to room temperature, a white wool-like product was deposited on the silicon substrate and the temperature of the cold zone was about 1050 C. The collected products were characterized by SEM (FESEM; JEOL JSM 6700F), HRTEM (JEOL 2010, at 200 kV), and EDX attached to the HRTEM. The dielectric properties of the products were measured by a dielectric relaxation spectrometer (Hioki 3531Z HiTESTER) The development of lab-on-a-chip technologies has attracted considerable attention in the past decade because miniaturized devices offer exciting opportunities for analytical, bio-, and synthetic chemistry, as well as for materials science. Downscaling allows the design of portable devices that are capable of performing complete analysis of samples, so-called total analysis systems (see a review by Jakeway et al.[1] ), reduces the amounts of reagents and speeds up processing and reaction times owing to reduced lengths in the device and the correspondingly shorter diffusion times. Finally, lowcost, mass-produced microfluidic chips can be readily adapted for massive parallel analysis or syntheses that work with ultrasmall quantities. A central challenge for downscaling remains the improvement of existing and the development of new device architectures that allow transport, separation, and analysis of reagents. In this communication, we present a novel and versatile approach to integrating semipermeable membranes with thicknesses down to 50 nm in soft lithographic structures.[2] We show that these membranes act as diffusion barriers for macromolecules, while they are permeable for lowmolecular-weight species, which makes them interesting for separation purposes. In addition, such a membrane can be used as an osmotic pressure sensor if its deflection resulting from a pressure difference is monitored. Here, we focus on the first aspect, investigating arrays of micrometer-sized cavities filled with entrapped macromolecules and covered with membranes that are permeable for low-molecular-weight spe-COMMUNICATIONS

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confidence: 91%

Filled Microcavity Arrays Produced by Polyelectrolyte Multilayer Membrane Transfer

et al. 2005

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“…The concentration of neuraminidase in the collected supernatant was determined by the UV-vis spectrometer. Figure 3 shows the release rates of neuraminidase from (PSS/PAH) 2 [21,29,30]. Our results are consistent with their findings that the release rate of proteins from polyelectrolyte microcapsules over the first hours is significantly higher than that over the following hours.…”

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confidence: 89%

Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro

Liu

Wang

Bai

et al. 2014

J. R. Soc. Interface.

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Microcapsules made of polyelectrolyte multilayers exhibit no or low toxicity, appropriate mechanical stability, variable controllable degradation and can incorporate remote release mechanisms triggered by various stimuli, making them well suited for targeted drug delivery to live cells. This study investigates interactions between microcapsules made of synthetic (i.e. polystyrenesulfonate sodium salt/polyallylamine hydrochloride) or natural (i.e. dextran sulfate/poly-L-arginine) polyelectrolyte and human umbilical vein endothelial cells with particular focus on the effect of the glycocalyx layer on the intake of microcapsules by endothelial cells. Neuraminidase cleaves N-acetyl neuraminic acid residues of glycoproteins and targets the sialic acid component of the glycocalyx on the cell membrane. Three-dimensional confocal images reveal that microcapsules, functionalized with neuraminidase, can be internalized by endothelial cells. Capsules without neuraminidase are blocked by the glycocalyx layer. Uptake of the microcapsules is most significant in the first 2 h. Following their internalization by endothelial cells, biodegradable DS/PArg capsules rupture by day 5; however, there is no obvious change in the shape and integrity of PSS/PAH capsules within the period of observation. Results from the study support our hypothesis that the glycocalyx functions as an endothelial barrier to cross-membrane movement of microcapsules. Neuraminidase-loaded microcapsules can enter endothelial cells by localized cleavage of glycocalyx components with minimum disruption of the glycocalyx layer and therefore have high potential to act as drug delivery vehicles to reach tissues beyond the endothelial barrier of blood vessels.

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“…1,2 Over the last decades, alternatives to common surfactants have been studied and developed. 2 Many approaches have been developed, such as pickering emulsions based on solid particles, [3][4][5] bolaamphiphiles, 6 microcapsules, 7,8 and surfactant-like peptides, 9 for water-in-oil, oil-in-water or even water-in-oil-in-water emulsions. 10 In addition, so-called pepfactants (long chain (21-mer) peptide surfactants) have been demonstrated to stabilize foams and emulsions at fluid-fluid interfaces in a stimuli-responsive 3 manner by changing the bulk solution conditions.…”

Section: Introductionmentioning

confidence: 99%

Enzymatically activated emulsions stabilised by interfacial nanofibre networks

et al. 2016

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This version is available at https://strathprints.strath.ac.uk/55388/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the ABSTRACTWe report on the on-demand formation of emulsions stabilized by interfacial nanoscale networks by biocatalytic self-assembly of Fmoc (9-fluorenylmethoxycarbonyl) dipeptide amphiphiles in aqueous/organic mixtures. The use of an alkaline phosphatase to transform phosphorylated precursors into self-assembling aromatic peptide amphiphiles (Fmoc-tyrosine-leucine, Fmoc-YL) provides a route to trigger self-assembly of nanofibrous networks and gels. In biphasic organic/aqueous systems, these networks form preferentially at the interface. This gives rise to the possibility of on-demand activation of emulsifying ability, producing switchable emulsions that may be activated by enzyme addition, even after storage of the biphasic mixture for several weeks. Experimental (Fluorescence and FTIR spectroscopy) and computational techniques (Atomistic Molecular Dynamics) are combined to show that the self-assembly process of Fmoc-YL occurs through aromatic interactions and hydrogen bonding to generate an interfacial nanofibrous network.

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Sustained Release Properties of Polyelectrolyte Multilayer Capsules

Cited by 353 publications

References 19 publications

Filled Microcavity Arrays Produced by Polyelectrolyte Multilayer Membrane Transfer

Filled Microcavity Arrays Produced by Polyelectrolyte Multilayer Membrane Transfer

Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro

Enzymatically activated emulsions stabilised by interfacial nanofibre networks

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