Sustained Release of Proteins from Electrospun Biodegradable Fibers

Chew, Sing Yian; Wen, Jie; Yim, Evelyn K.F.; Leong, Kam W.

doi:10.1021/bm0501149

Cited by 530 publications

(370 citation statements)

References 34 publications

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“…collagen, silk fibroin, and fibrinogen) and synthetic polymers (e.g. PGA, PLLA, PLGA, and PCL) have been processed into fine nonwoven fabrics for tissue engineering research [48,[50][51][52][53][54][55][56][57][58][59][60]. Various cells have been reported to attach, proliferate, and differentiate into or maintain their functional phenotypes on these electrospun nano-fibrous materials [48,54,58,59,61,62].…”

Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,181

815

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Tissue engineering and regenerative medicine is an exciting research area that aims at regenerative alternatives to harvested tissues for transplantation. Biomaterials play a pivotal role as scaffolds to provide three-dimensional templates and synthetic extracellular-matrix environments for tissue regeneration. It is often beneficial for the scaffolds to mimic certain advantageous characteristics of the natural extracellular matrix, or developmental or would healing programs. This article reviews current biomimetic materials approaches in tissue engineering. These include synthesis to achieve certain compositions or properties similar to those of the extracellular matrix, novel processing technologies to achieve structural features mimicking the extracellular matrix on various levels, approaches to emulate cell-extracellular matrix interactions, and biologic delivery strategies to recapitulate a signaling cascade or developmental/would-healing program. The article also provides examples of enhanced cellular/tissue functions and regenerative outcomes, demonstrating the excitement and significance of the biomimetic materials for tissue engineering and regeneration.

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Section: Electrospinningmentioning

confidence: 99%

Biomimetic materials for tissue engineering

Peter

2008

Advanced Drug Delivery Reviews

1,181

815

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“…Organic solvent and water are subsequently removed by freeze drying leaving a solid porous polymer with porosity up to 90% [96]. This technique has been applied to many biocompatible polymers such as PLA, PGA, PLAGA, PCL, chitosan and alginate [97][98][99][100]. Despite its versatility, freeze drying is a time and energy consuming method that takes several days to completely eliminate solvents.…”

Section: Freeze Dryingmentioning

confidence: 99%

“…However, most of these studies concern the immobilization of such biomolecules at the fibre surface [94][95][96]. Leong and coworkers incorporated GFs that were intended to promote nerve regeneration into ES fibres of caprolactone-ethylethylene phosphate copolymer [97,98]. In other studies, GF have been incorporated into fibres by using coaxial ES [99,100].…”

Section: "Composite" Electrospun Scaffoldsmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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“…Electrospun fibers can encapsulate bioactive drugs and proteins [10][11][12] and may be used as tissue scaffolds for direct in vivo applications [9]. Inclusion of aligned electrospun fibers in a nerve conduit could enhance sciatic nerve regeneration over a 15 mm critical size defect in rats after 3 months post-implantation [8].…”

Section: Introductionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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Sustained Release of Proteins from Electrospun Biodegradable Fibers

Cited by 530 publications

References 34 publications

Biomimetic materials for tissue engineering

Biomimetic materials for tissue engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

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