Sustained release of nanosized complexes of polyethylenimine and anti-TGF-β2 oligonucleotide improves the outcome of glaucoma surgery

Santos, Ana Lúcia Gomes dos; Bochot, Amélie; Doyle, Aoife; Tsapis, Nicolas; Siepmann, Juergen; Siepmann, Florence; Schmaler, Jeannette; Besnard, Madeleine; Behar‐Cohen, Francine

doi:10.1016/j.jconrel.2006.02.010

Cited by 87 publications

(40 citation statements)

References 21 publications

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“…Rabbits have been widely used to study ocular pharmacokinetics of drugs. [42][43][44][45][46] Although some differences between the rabbit and human eye do exist, for example, volume of vitreous humor and minimal neural retinal vasculature, pharmacokinetic behavior of drugs in this model extrapolates well with that observed in humans. However, the structural differences need to be kept in mind while evaluating the data.…”

Section: Discussionmentioning

confidence: 73%

Evaluation of the Intravenous and Topical Routes for Ocular Delivery of Hesperidin and Hesperetin

Srirangam

Hippalgaonkar

Avula

et al. 2012

Journal of Ocular Pharmacology and Therapeutics

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Purpose: The objective of this study was to determine the ocular bioavailability of hesperidin and hesperetin, especially with respect to their distribution into the posterior segment of the eye, following systemic and topical administration in rabbits. Methods: Hesperidin and hesperetin were administered either intravenously or topically to male New Zealand white (NZW) rabbits. Vitreous humor and plasma samples were collected after intravenous administration and analyzed to estimate the concentrations of the parent compounds and their metabolites. Ocular tissue concentrations, obtained on topical administration of hesperidin and hesperetin, were also determined. Results: In the systemic circulation, hesperidin and hesperetin were rapidly metabolized into their glucuronides, which are extremely hydrophilic in nature. Vitreal samples did not demonstrate any detectable levels of hesperidin/hesperetin following intravenous administration. Topical administration produced significant concentrations of hesperidin/hesperetin in all the ocular tissues tested at the 1 and 3 hours time points postdosing, with hesperetin showing higher levels compared to hesperidin. However, only low levels were generated in the vitreous humor. Inclusion of a penetration enhancer, benzalkonium chloride (BAK), improved the back-ofthe-eye hesperetin levels. Conclusions: Ocular delivery of hesperidin/hesperetin via the systemic route does not seem to be feasible considering the rapid generation of the hydrophilic metabolites. Topical application appears to be more promising and needs to be further developed/refined.

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Section: Discussionmentioning

confidence: 73%

Evaluation of the Intravenous and Topical Routes for Ocular Delivery of Hesperidin and Hesperetin

Srirangam

Hippalgaonkar

Avula

et al. 2012

Journal of Ocular Pharmacology and Therapeutics

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“…Nearly all of these reports are based on the absorption of CpG onto preformed positive particles except for few works which tested the adjuvant effect inside the polymeric matrix with more [25] or less [45] success. In another context, some studies have applied for the complexation of CpG oligonucleotides or CpG DNA with positive molecules (such as PEI [31] or DOTAP) to modify the intracellular uptake and route with negative or positive effect in their stimulatory activity. In the current study, we investigated the immune response induced by the co-delivery of CpG-chitosan complexes (not characterized in this research work) and OVA in non-charged PLGA microspheres.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the association of CpG sequences with positive molecules has been described as a successful strategy to load both molecules into the same particle [31,32]. The milestone of this idea is the counterbalancing of the CpG negative charges and the reduction of its hidrophylicity.…”

Section: Introductionmentioning

confidence: 99%

Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology

Román

Irache

Gómez

et al. 2008

European Journal of Pharmaceutics and Biopharmaceutics

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“…Particle porosity has been attributed to high osmotic pressure inside the internal aqueous phase (25). Addition of NaCl to the external phase seems to reduce the osmotic pressure difference between the internal and external aqueous phases (25,26). Particles with a porous surface lead to an enhanced initial burst release.…”

Section: Discussionmentioning

confidence: 99%

Antibody Response is Differentially Influenced by PLGA-PAD4 Particle Characteristics

Manish

Bhatnagar

2017

Int J Infect

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Background: Anthrax, as a zoonotic disease, is considered a major bioterror threat. Development of effective prophylactic approaches is needed to combat this disease. The protective antigen domain 4 (PAD4) has been extensively studied as a vaccine candidate against anthrax. Previously, we showed that PAD4 can be successfully encapsulated in poly (lactide-co-glycolide) (PLGA)-based particulate systems. Objectives: We aimed to determine if there is an intricate relationship between the parameters of PLGA particulate process and the associated immunogenicity. Methods: In this study, PLGA (50:50) and PLGA (85:15) with a similar molecular weight were used as the polymer variants. Polymer variants were evaluated using 3 adaptations of w/o/w solvent evaporation method. Six variants of PLGA-PAD4 particulate systems were developed and studied for size, antigen content, and immunogenicity. Results: NaCl in the external aqueous phase was required to achieve nonporous and spherical microparticles. Particle size was found to be dependent on the applied methods, and there was no significant difference in size by varying the PLGA compositions. PLGA variants showed a differential behavior with antigen encapsulation in the evaluated methods. The hydrophobic particulate

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Sustained release of nanosized complexes of polyethylenimine and anti-TGF-β2 oligonucleotide improves the outcome of glaucoma surgery

Cited by 87 publications

References 21 publications

Evaluation of the Intravenous and Topical Routes for Ocular Delivery of Hesperidin and Hesperetin

Evaluation of the Intravenous and Topical Routes for Ocular Delivery of Hesperidin and Hesperetin

Co-encapsulation of an antigen and CpG oligonucleotides into PLGA microparticles by TROMS technology

Antibody Response is Differentially Influenced by PLGA-PAD4 Particle Characteristics

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