Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

Mori, Arianna De; Hafidh, Meena; Mele, Natalia; Yusuf, Rahmi; Cerri, Guido; Gavini, Elisabetta; Tozzi, Gianluca; Barbu, Eugen; Conconi, Mariateresa; Draheim, Roger Russell; Roldo, Marta

doi:10.3390/pharmaceutics11030116

Cited by 25 publications

(25 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AgNWs were synthetized using the polyol method, as previously described. 14 AgNWs, with an average length of 5 μm and an average diameter of 99 nm, were obtained. 14…”

Section: Methodsmentioning

confidence: 99%

Antibacterial PMMA Composite Cements with Tunable Thermal and Mechanical Properties

et al. 2019

Self Cite

View full text Add to dashboard Cite

PMMA-based cements are the most used bone cements in vertebroplasty and total hip arthroplasty. However, they present several drawbacks, including susceptibility to bacterial infection, monomer leakage toxicity, and high polymerization temperature, which can all lead to damage to the surrounding tissues and their failure. In the present study, silver nanowires (AgNWs) have been introduced to bestow antibacterial properties; chitosan (CS) to promote porosity and to reduce the polymerization temperature, without negatively affecting the mechanical performance; and methacryloyl chitosan (CSMCC) to promote cross-linking with methyl methacrylate (MMA) and reduce the quantity of monomer required for polymerization. Novel PMMA cements were formulated containing AgNWs (0 and 1% w/w) and CS or CSMCC at various concentrations (0, 10, 20, and 30% w/w), testing two different ratios of powder and MMA (P/L). Mechanical, thermal, antibacterial, and cytotoxic properties of the resulting composite cements were tested. Cements with concentrations of CS > 10% presented a significantly reduced polymerization temperature. The mechanical performances were affected for concentrations > 20% with a P/L concentration equal to 2:1. Concentrations of AgNWs as low as 1% w/w conferred antimicrobial activity against S. aureus, whereas biofilm formation on the surface of the cements was increased when CS was included in the preparation. The combination of CS and AgNWs allowed a higher concentration of Ag+ to be released over time with enhanced antimicrobial activity. Inclusion of AgNWs did not affect cell viability on the scaffolds. In conclusion, a combination of CS and AgNWs may be beneficial for reducing both polymerization temperature and biofilm formation, without significantly affecting mesenchymal stem cell proliferation on the scaffolds. No advantages have been noticed as a result of the reducing P/L ratio or using CSMCC instead of CS.

show abstract

“…AgNWs were synthetized using the polyol method, as previously described. 14 AgNWs, with an average length of 5 μm and an average diameter of 99 nm, were obtained. 14…”

Section: Methodsmentioning

confidence: 99%

Antibacterial PMMA Composite Cements with Tunable Thermal and Mechanical Properties

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…AgNWs were synthesized via the polyol method, as previously described [43]. AgNWs-CNT were synthetized in the presence of CNTs with a slightly modified method.…”

Section: Synthesis Of Agnws and Compositesmentioning

confidence: 99%

“…This step was repeated twice. The solid pellet obtained was then washed and stored as previously described [43].…”

Section: Synthesis Of Agnws and Compositesmentioning

confidence: 99%

“…The set parameters for the analysis were as follows: 1450 W for plasma power, 30 rpm for pump speed, 13 L/min for coolant flow and 0.75 L/min for nebulizer flow. Ag + release from nanoparticles was determined as previously reported [43]. In order to determine whether either AgNWs or CNTs possessed any autofluorescence, freeze-dried NPs were suspended in deionized water and sonicated to provide a homogeneous suspension.…”

Section: Characterization Of Agnws and Compositesmentioning

confidence: 99%

“…The effect of the prepared samples on bacterial growth kinetics was assessed as previously described [43].…”

Section: Bacterial Growth Kineticsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Antibacterial and Cytotoxicity Properties of Silver Nanowires and Their Composites with Carbon Nanotubes for Biomedical Applications

Mori

Jones

Cretella

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

In this work, we prepared silver nanowires (AgNWs) via the polyol method in the presence or absence of single wall carbon nanotubes (CNTs) and tested their physicochemical, antibacterial and cytotoxic properties. Results showed that the introduction of CNTs lead to the formation of AgNWs at lower temperature, but the final product characteristics of AgNWs and AgNWs-CNT were not significantly different. AgNWs exhibited antibacterial properties against all the studied bacterial species via the formation of oxygen reactive species (ROS) and membrane damage. Furthermore, AgNWs exhibited a dose-dependent and time-dependent toxicity at concentrations ≥ 10 µg/mL. Fibroblasts appeared to be more resistant than human colorectal adenocarcinoma (Caco-2) and osteoblasts to the toxicity of AgNWs. The cytotoxicity of AgNWs was found to be related to the formation of ROS, but not to membrane damage. Overall, these results suggest that AgNWs are potential antibacterial agents against E. coli, S. aureus, MRSA and S. saprophyticus, but their dosage needs to be adjusted according to the route of administration.

show abstract

Biomaterials against Bone Infection

Vallet‐Regí

Lozano

González

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

Cited by 25 publications

References 46 publications

Antibacterial PMMA Composite Cements with Tunable Thermal and Mechanical Properties

Antibacterial PMMA Composite Cements with Tunable Thermal and Mechanical Properties

Evaluation of Antibacterial and Cytotoxicity Properties of Silver Nanowires and Their Composites with Carbon Nanotubes for Biomedical Applications

Biomaterials against Bone Infection

Contact Info

Product

Resources

About