“…20,21 Western blot Cells were exposed to epidermal growth factor (EGF, 100 ng ml À1 ) for 5 min (Roche Diagnostics, Penzberg, Germany). Western blots were carried out with total protein extracted from cells as already described using monoclonal antibody directed against PTEN, pPTEN, P53, AKT, phosphorylated-AKT (pAKT), GSK3b, pGSK3b, P70S6K, pP70S6K, ERK1/2, pERK1/2, BAX and tubulin 22 (Table 1).…”