Sustained and Extended Release with Structural and Activity Recovery of Lysozyme from Complexes with Sodium (Sulfamate Carboxylate) Isoprene/Ethylene Oxide Block Copolymer

Abstract

The complexation of lysozyme and sodium (sulfamate carboxylate) isoprene/ethylene oxide (SCIEO) at pH = 7.4 and the release of lysozyme from the complexes in the presence of NaCl were investigated. Through electrostatic and hydrophobic interactions, lysozyme and SCIEO form stable complex nanoparticles. The complexation partially disturbs the structure of lysozyme. Some of the hydrophobic residues of lysozyme are exposed to bind with SCIEO. The complexation leads to loss of most of the lysozyme activity. In the presence of NaCl, lysozyme can be released from the complexes. The released lysozyme molecules recover their native structure and activity completely. In the condition of physiological pH and ionic strength, a sustained and extended release of lysozyme was achieved.

Interaction between lysozyme and colloidal poly(NIPAM-co-acrylic acid) microgels

“…The propensity of lysozyme to self-associate makes it interesting as a model protein since many other proteins also have this property. At the same time it is a well characterized small (M w = 14.5 kD) globular molecule (4.5 Â 3.0 Â 3.0 nm) [71], known to be structurally stable under a wide range of conditions, also in complexes with linear polyelectrolytes [45,72,73], and its phase behaviour in saline solutions, including conditions for gelation, has been investigated [71,74,75]. The present study constitutes a further downscale of the lysozyme-microgel system, to microgels of roughly 1 lm in diameter, synthesized by emulsion polymerization.…”

Complex coacervate‐based materials for biomedicine

“…Moving beyond the mere use of biological materials, researchers have also drawn inspiration from biological processes such as DNA–histone binding, heparin–growth factor interactions, and intracellular protein–RNA granules . Similarly, coacervate‐based materials can be designed to respond to a variety of stimuli, including ionic strength, changes in pH, redox chemistries, temperature, and light, for use in sensing or drug delivery applications . This review is designed to provide an overview of complex coacervation, with an emphasis on the use of such materials for applications in the broad field of biomedicine.…”

PEGylation to Improve Protein Stability During Melt Processing

“…[5] Depot formulations are advantageous because proteins are protected from in vivo degradation and the release profile can be sustained and controlled over time based on the polymer composition. [6] Current protein encapsulation methods used to create microspheres, [7][8] electrospun fibers, [9][10] and hydrogels [11]–[13] have seen great progress both in vitro and in vivo. These techniques, though, often involve co-dissolution steps where the protein is dissolved or dispersed into organic media alongside polymer excipients and surfactants.…”