Sustainable malaria control: transdisciplinary approaches for translational applications

Birkholtz, Lyn‐Marié; Bornman, Riana; Focke, Walter Wilhelm; Mutero, Clifford Maina; Jager, Christiaan De

doi:10.1186/1475-2875-11-431

Cited by 19 publications

(18 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mosquito-borne diseases remain a persistent threat to over half of the world’s population, and their range has been spreading due to globalization, urbanization, and climate change 32 – 34 . Current preventative strategies center around controlling the vectors of these diseases; most often, this involves the use of bed nets and insecticides 3 , 35 , 36 . However, many mosquito species have evolved resistances to commonly used insecticides 37 and some have even altered their biting behavior to gain access to humans at times of the day when bed nets are not being used 38 .…”

Section: Discussionmentioning

confidence: 99%

Blood meal-induced inhibition of vector-borne disease by transgenic microbiota

et al. 2018

View full text Add to dashboard Cite

Vector-borne diseases are a substantial portion of the global disease burden; one of the deadliest of these is malaria. Vector control strategies have been hindered by mosquito and pathogen resistances, and population alteration approaches using transgenic mosquitos still have many hurdles to overcome before they can be implemented in the field. Here we report a paratransgenic control strategy in which the microbiota of Anopheles stephensi was engineered to produce an antiplasmodial effector causing the mosquito to become refractory to Plasmodium berghei. The midgut symbiont Asaia was used to conditionally express the antiplasmodial protein scorpine only when a blood meal was present. These blood meal inducible Asaia strains significantly inhibit pathogen infection, and display improved fitness compared to strains that constitutively express the antiplasmodial effector. This strategy may allow the antiplasmodial bacterial strains to survive and be transmitted through mosquito populations, creating an easily implemented and enduring vector control strategy.

show abstract

Section: Discussionmentioning

confidence: 99%

Blood meal-induced inhibition of vector-borne disease by transgenic microbiota

et al. 2018

View full text Add to dashboard Cite

show abstract

“…MBVs are also transmitted between male and female mosquitoes or from a female to the offspring. Furthermore, malaria and lymphatic filariasis, transmitted by hematophagous arthropods, are still the most prevalent mosquito-borne parasitic diseases in humans [ 13 , 14 ]. A continuous effort to monitor the MBVs is critical to prevent sudden outbreaks [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, malaria parasites and lymphatic filariae, borne by mosquitoes, are currently the most common parasites in the world [ 13 , 14 ]. Malaria and lymphatic filariasis have been considered as the world’s highest priority control and eradication parasitic diseases due to their wide prevalence [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

et al. 2017

View full text Add to dashboard Cite

BackgroundMosquito-borne viruses (MBVs) and parasites (MBPs) are transmitted through hematophagous arthropods-mosquitoes to homoiothermous vertebrates. This study aims at developing a detection method to monitor the spread of mosquito-borne diseases to new areas and diagnose the infections caused by MBVs and MBPs.MethodsIn this assay, an ultra-sensitive chemiluminescence (CL) detection method was developed and used to simultaneously detect 19 common MBVs and MBPs. In vitro transcript RNA, virus-like particles (VLPs), and plasmids were established as positive or limit of detection (LOD) reference materials.ResultsMBVs and MBPs could be genotyped with high sensitivity and specificity. The cut-off values of probes were calculated. The absolute LODs of this strategy to detect serially diluted in vitro transcribed RNAs of MBVs and serially diluted plasmids of MBPs were 102–103copies/μl and 101–102copies/μl, respectively. Further, the LOD of detecting a strain of pre-quantified JEV was 101.8–100.8PFU/ml, fitted well in a linear regression model (coefficient of determination = 0.9678).ConclusionsUltra-sensitive CL imaging DNA hybridization was developed and could simultaneously detect various MBVs and MBPs. The method described here has the potential to provide considerable labor savings due to its ability to screen for 19 mosquito-borne pathogens simultaneously.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-1975-1) contains supplementary material, which is available to authorized users.

show abstract

“…Mosquitoes are insect vectors of deadly diseases such as malaria, dengue fever, Zika virus, lymphatic filariasis and West Nile fever. They infect hundreds of millions of people, causing immense suffering and up to a million deaths per annum 1 . These disease transmissions can be reduced by using, for example, topical mosquito repellents that prevent or at least reduce contact between the blood-seeking female mosquitoes and humans 2 .…”

Section: Introductionmentioning

confidence: 99%

A promising azeotrope-like mosquito repellent blend

Izadi

Focke

Asaadi

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Topical repellents play a key role in reducing the outdoor transmission of mosquito-borne diseases by reducing human-vector contact. Excellent repellents are available, but there is always room for improvement. This article reports on a particularly effective binary repellent blend of ethyl butylacetylaminopropionate and nonanoic acid. A composition containing 25 mol% of the acid exhibits negative pseudo-azeotrope behaviour at 50 °C, meaning that the liquid vapour pressure is lower than that of the parent compounds and evaporation occurs without a change in the liquid composition. In tests performed using the South African Medical Research Council’s cup-on-arm procedure, this mixture provided better protection for a longer time than the “gold standard of mosquito repellents”, namely N,N-diethyl-m-toluamide, commonly known as DEET.

show abstract

Sustainable malaria control: transdisciplinary approaches for translational applications

Cited by 19 publications

References 59 publications

Blood meal-induced inhibition of vector-borne disease by transgenic microbiota

Blood meal-induced inhibition of vector-borne disease by transgenic microbiota

Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

A promising azeotrope-like mosquito repellent blend

Contact Info

Product

Resources

About