Sustainable Green Analytical Chemistry: Spectrophotometric Method Development of Dapagliflozin and Omeprazole by Using Eco-Friendly Solvent

Abstract

The present research’s aim was to develop analytical method utilizing ecologically suitable solvent which enhances solubility of analyte, sensitivity of the method etc. An analytical method was developed for the estimation of dapagliflozin (DGZ) and omeprazole (OPZ) by using aqueous 0.1 N HCl on the UV-VIS spectrophotometer. Wavelengths 223 nm and 282.5 nm were set to measure absorbance of DGZ and OPZ respectively. The results of different spectral characteristic techniques were examined to select the parameters and the design was validated against the ICH Q 2 R1 regulatory guidelines. The linearity of the drug was determined at a concentration of 1 to 50μg/ml and 1 to 32μg/ml for DGZ and OPZ respectively. The accuracy was found within acceptable limit with standard deviation 1.1874 to 6.4984 for DGZ and 0.1401 to 0.9843 for OPZ; and the assay study data was found 99.77 % for DGZ and 97.92 % for OPZ. The stability study of the method was performed out by minute variation in the wavelength, scan speed. The developed method is rigid, robust and efficient for the estimation of DGZ and OPZ from their respective dosage form. The effort was made to develop green or eco-friendly analytical method utilizing hydrotropic solvent for water insoluble drug dapagliflozin and omeprazole. KEYWORDS: Green method, Dapagliflozin, Omeprazole, analytical method, eco-friendly solvent, UV spectroscopy.