Sustainable Bombyx mori's silk fibroin for biomedical applications as a molecular biotechnology challenge: A review

Bitar, Lara; Isella, Benedetta; Bertella, Francesca; Bettker Vasconcelos, Carolina; Harings, Jules; Kopp, Alexander; van der Meer, Yvonne; Vaughan, Ted J.; Bortesi, Luisa

doi:10.1016/j.ijbiomac.2024.130374

Cited by 4 publications

(1 citation statement)

References 143 publications

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“…Sericins have a molecular weight range between 20 and 310 kDa [157,158] and are comprised of 44% glycine, 29% alanine, and 11% serine [159]. A bacterium bearing the genes from B. mori has been used to overexpress fibrion [101,160,161] and sericin proteins [162,163]. There are two different types of silk fibroin in its solid state.…”

Section: Silk Fibroinmentioning

confidence: 99%

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Nawaz,

Gu,

Gibbons

et al. 2024

Biomimetics

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The sophisticated, elegant protein-polymers designed by nature can serve as inspiration to redesign and biomanufacture protein-based materials using synthetic biology. Historically, petro-based polymeric materials have dominated industrial activities, consequently transforming our way of living. While this benefits humans, the fabrication and disposal of these materials causes environmental sustainability challenges. Fortunately, protein-based biopolymers can compete with and potentially surpass the performance of petro-based polymers because they can be biologically produced and degraded in an environmentally friendly fashion. This paper reviews four groups of protein-based polymers, including fibrous proteins (collagen, silk fibroin, fibrillin, and keratin), elastomeric proteins (elastin, resilin, and wheat glutenin), adhesive/matrix proteins (spongin and conchiolin), and cyanophycin. We discuss the connection between protein sequence, structure, function, and biomimetic applications. Protein engineering techniques, such as directed evolution and rational design, can be used to improve the functionality of natural protein-based materials. For example, the inclusion of specific protein domains, particularly those observed in structural proteins, such as silk and collagen, enables the creation of novel biomimetic materials with exceptional mechanical properties and adaptability. This review also discusses recent advancements in the production and application of new protein-based materials through the approach of synthetic biology combined biomimetics, providing insight for future research and development of cutting-edge bio-inspired products. Protein-based polymers that utilize nature’s designs as a base, then modified by advancements at the intersection of biology and engineering, may provide mankind with more sustainable products.

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Section: Silk Fibroinmentioning

confidence: 99%

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Nawaz,

Gu,

Gibbons

et al. 2024

Biomimetics

View full text Add to dashboard Cite

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Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

Lin,

Li,

Wang

et al. 2024

Adv Funct Materials

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The creation of functional hydrogels with robust load‐bearing capacity adaptable to complex tissue regeneration remains challenging. Silk fibroin (SF) is a natural biomaterial with excellent mechanical strength and cell adhesion capacity, possessing tremendous potential to solve the aforementioned dilemma. The excellent biodegradability and biocompatibility and the molecular structure with multiple modifiable moieties provide opportunities for the injectability and multifunctionality of hydrogels. Furthermore, the incorporation of other polymers or active ingredients can improve the basic properties, confer biological activities and pharmacological effects, and provide the prerequisites for hydrogels to fulfill specific requirements. Therefore, SF‐based hydrogels are widely applied in tissue repair and regeneration, especially in bone, skin, nerve, liver, myocardium, and cornea, which have effectively addressed the challenges of scaffolds' lack of cell adhesion sites, excessive degradation, as well as the lack of efficient, long‐lasting antimicrobial properties. This review outlines the properties of SF, discusses the types of materials commonly used in the preparation of hydrogels and their characteristics, and describes the current construction methods of SF hydrogels. Simultaneously, recent advancements in applying SF‐based multifunctional hydrogels within biological tissues are explored, with a focus on their role in tissue repair, highlighting the repair mechanism of SF based on bone and skin.

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Hybrid construction of tissue-engineered nerve graft using skin derived precursors induced neurons and Schwann cells to enhance peripheral neuroregeneration

Guo,

Zhu,

et al. 2024

Materials Today Bio

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Sustainable Bombyx mori's silk fibroin for biomedical applications as a molecular biotechnology challenge: A review

Cited by 4 publications

References 143 publications

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications

Multifunctional Hydrogel Based on Silk Fibroin Promotes Tissue Repair and Regeneration

Hybrid construction of tissue-engineered nerve graft using skin derived precursors induced neurons and Schwann cells to enhance peripheral neuroregeneration

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