Sustainable biodiesel production from oleaginous yeasts utilizing hydrolysates of various non-edible lignocellulosic biomasses

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

“…Concerning strains of R. kratochvilovae (FMCC Y 70 and FMCC Y 71 ) did not produce significant SCO quantities under the present culture conditions, in contrast to literature reports indicating that at least some Rhodotorula (previous name Rhodosporidium) kratochvilovae strains (i.e. strain HIMPA1) can be efficient SCO producers during growth on hydrophilic substrates (Patel et al 2014(Patel et al , 2015(Patel et al , 2016(Patel et al , 2017. Other species of the genus Rhodosporidium, especially the ones of the species R. toruloides, have also shown a very good ability in lipid accumulation when grown in Gly (Uc ßkun Kiran et al 2013;Yang et al 2014;Tchakouteu et al 2015b).…”

“…In contrast to these results, another new Debaryomyces isolate (D. etchellsii strain BM1 isolated from olive-mill wastewaters) during its cultivation on sugar-or Gly-based media, presented the typical pattern of oil accumulation after nitrogen depletion from the medium (Arous et al 2015(Arous et al , 2016a, presenting also remarkable ascosporogenesis (Arous et al 2015). The highest SCO production in the current investigation was reported by R. glutinis NRRL YB-252 (L max = 7Á2 g l À1 , Y L/X = 38Á2% w/w), that are values quite interesting and comparable to several ones reported in the literature concerning growth of 'red' yeasts (genera Rhodosporidium or Rhodotorula) on various low-cost hydrophilic carbon sources (Saenge et al 2011;Uc ßkun Kiran et al 2013;Patel et al 2014Patel et al , 2015Patel et al , 2016Patel et al , 2017Uprety et al 2017a. Lipid produced by R. glutinis NRRL YB-252 cultivated in high Gly 0 concentrations presented high quantities of oleic acid (>70% w/w), suggesting the use of Gly as substrate in order for lipid presenting excellent composition as regards its potential transformation into second generation biodiesel or its utilization as starting fatty material amenable for various lubricant applications (Uprety et al 2017a;Tsakraklides et al 2018) to be created.…”

Lipids from yeasts and fungi: physiology, production and analytical considerations

“…Moreover, in the last few years there has been a great upsurge of interest in studies related with various aspects of SCO production, due to potential industrial applications that SCOs may have. Therefore, investigations dealing with the biochemistry, molecular biology, physiology and biochemical engineering of SCO production process present a continuously increasing interest for the microbiological community worldwide (for reviews see: Ratledge and Wynn 2002;Beopoulos et al 2009aBeopoulos et al , 2009bFakas et al 2009a;Huang et al 2013;Meeuwse et al 2013;Xu et al 2013;Muniraj et al 2015a;Patel et al 2016;Bharathiraja et al 2017). In fact, SCOs can be employed as substitutes of expensive lipids rarely found in the Plant or Animal Kingdom (e.g.…”

“…sugars, glycerol, etc.) are considered as potential and important candidates for the production of this lipid that would result in the generation of the second-generation lipid-based biofuels (Huang et al 2013;Meeuwse et al 2013;Xu et al 2013;Koutinas et al 2014a;Muniraj et al 2015a;Patel et al 2016Patel et al , 2017aQin et al 2017). It is evident that concerning the production of liquid biofuels with the aid of oleaginous micro-organisms, only the hydrophilic carbon sources should be used as substrates for this purpose; it would evidently not make sense to proceed to fermentation of a fatty substrate in order to create cellular lipid that would subsequently be converted into biofuel (biodiesel or renewable diesel), while the initial fat substrate could have already been directly converted into biofuel without the step of fermentation being implicated.…”

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

“…Glucose is most commonly used for cultivation of oleaginous microorganisms, but various plant-derived mono-and disaccharides can be used as carbon sources instead (Zhang et al, 2016), which broadens the spectrum of potential feedstocks. Lignocellulosic biomass, which is mainly composed of the polysaccharides cellulose and hemicellulose and the polyaromatic compound lignin, is the most abundant renewable and non-edible resource in the world, which can be used as a carbon source to reduce the production cost of microbial lipids (Patel et al, 2016). Several oleaginous yeast species have thus been cultivated on lignocellulose-based hydrolysates (Whiffin et al, 2016).…”