Kombucha is a well-known fermented beverage that offers various nutritional and health benefits to humans. Various substrates have been considered for kombucha fermentation, including herbs, fruits, flowers, and vegetables, to enhance flavors, odor, and nutritional value. In this study we examined the potential of cascara or coffee husk, as a substrate for kombucha production. Our results indicated that a combination of cascara and a sucrose concentration of 10g/L was appropriate for the fermentation of kombucha. The cascara kombucha achieved a low pH of 2.69 after a 14-day of fermentation, which corresponds to microbiological safety requirements. Additionally, fermentation increased polyphenols, flavonoids, and total acids content, resulting in antioxidant, antimicrobial, and prebiotic activities that promote a healthy gut microbiota. Furthermore, 14 days of fermentation has been demonstrated for achieving a balanced sensory profile, including sourness, sweetness, and odor, as well as enhancing the health-promoting nutrient accumulation. These findings emphasize the potential health benefits of cascara kombucha, making it an appealing beverage for consumers at the same time, while also repurposing the coffee industry by-products, contributing to sustainable and environmentally friendly development.