Sustainability of strategies for Ostrinia nubilalis management in Northern Italy: Potential impact on beneficial arthropods and aflatoxin contamination in years with different meteorological conditions

Magagnoli, Serena; Lanzoni, Alberto; Masetti, Antonio; Depalo, Laura; Albertini, Marco; Ferrari, Roberto; Spadola, Giorgio; Degola, Francesca; Restivo, Francesco Maria; Burgio, Giovanni

doi:10.1016/j.cropro.2020.105529

Cited by 10 publications

(13 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aflatoxin-contaminated peanuts pollute the environment and cause the serious economic damage [10]. Despite remarkable improvements in the technologies of harvesting and storage processing, the contamination of aflatoxins is still a problem to be solved in the peanut industry [11]. Various physical, chemical, and biological methods have been developed to solve the problem of aflatoxin pollution [12].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts

Yang

Xiao

Shen

et al. 2021

Toxins

View full text Add to dashboard Cite

Aflatoxins seriously threaten the health of humans and animals due to their potential carcinogenic properties. Enzymatic degradation approach is an effective and environmentally friendly alternative that involves changing the structure of aflatoxins. In this study, Trametes versicolor aflatoxin B1-degrading enzyme gene (TV-AFB1D) was integrated into the genome of Pichia pastoris GS115 by homologous recombination approach. The recombinant TV-AFB1D was expressed in engineering P. pastoris with a size of approximately 77 kDa under the induction of methanol. The maximum activity of TV-AFB1D reached 17.5 U/mL after the induction of 0.8% ethanol (v/v) for 84 h at 28 °C. The AFB1 proportion of 75.9% was degraded using AFB1 standard sample after catalysis for 12 h. In addition, the AFB1 proportion was 48.5% using AFB1-contaminated peanuts after the catalysis for 18 h at 34 °C. The recombinant TV-AFB1D would have good practical application value in AFB1 degradation in food crops. This study provides an alternative degrading enzyme for the degradation of AFB1 in aflatoxin-contaminated grain and feed via enzymatic degradation approach.

show abstract

Section: Introductionmentioning

confidence: 99%

Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts

Yang

Xiao

Shen

et al. 2021

Toxins

View full text Add to dashboard Cite

show abstract

“…The AFB 1 determination was carried out by high-pressure liquid chromatography (HPLC), as reported by Magagnoli et al [ 23 ] following the Kobra ® Cell method (R-Biopharm Rhone, Ltd., Glasgow, UK). Briefly: 20 g of maize kernels were grounded in a blender with 100 mL 70% MetOH, filtered through a paper filter, diluted 1:5 in Milli-Q water, and filtered with a microfiber filter (1.5 μm, VICAM, Watertown, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Validation and Ecological Niche Investigation of a New Fungal Intraspecific Competitor as a Biocontrol Agent for the Sustainable Containment of Aflatoxins on Maize Fields

Spadola

Giannelli

Magagnoli

et al. 2022

JoF

Self Cite

View full text Add to dashboard Cite

Crop yield and plant products quality are directly or indirectly affected by climate alterations. Adverse climatic conditions often promote the occurrence of different abiotic stresses, which can reduce or enhance the susceptibility to pests or pathogens. Aflatoxin producing fungi, in particular, whose diffusion and deleterious consequences on cereals commodities have been demonstrated to highly depend on the temperature and humidity conditions that threaten increasingly larger areas. Biological methods using intraspecific competitors to prevent fungal development and/or toxin production at the pre-harvest level are particularly promising, even if their efficacy could be affected by the ecological interaction within the resident microbial population. A previously characterized Aspergillus flavus atoxigenic strain was applied in two maize fields to validate its effectiveness as a biocontrol agent against aflatoxin contamination. At one month post-application, at the harvest stage, its persistence within the A. flavus population colonizing the maize kernels in the treated area was assessed, and its efficacy was compared in vitro with a representation of the isolated atoxigenic population. Results proved that our fungal competitor contained the aflatoxin level on maize grains as successfully as a traditional chemical strategy, even if representing less than 30% of the atoxigenic strains re-isolated, and achieved the best performance (in terms of bio-competitive potential) concerning endogenous atoxigenic isolates.

show abstract

“…The maize borer, Ostrinia nubilalis, the maize earworm, Heliothis zea, and the fall armyworm, Spodoptera frugiperda, have been identified as the major insects implicated in A. flavus infection and subsequent aflatoxin contamination of preharvest maize (Widstrom, 1979;Windham et al, 1999). When insect damage occurred simultaneously with favourable climatic conditions, such as drought stress and high temperature, high aflatoxin concentrations were usually found in maize grains (Magagnoli et al, 2021) techniques will reduce formation of holes and damage to the cobs which, in turn, will reduce the entry points for the fungus Sétamou et al, 1998). Thus, the application of registered insecticides especially when drought and heat stress frequently occur during the reproductive stages of the plants, can mitigate the risk of fungal growth.…”

Section: Insect Controlmentioning

confidence: 99%

Review of good agricultural practices for smallholder maize farmers to minimise aflatoxin contamination

Xu¹,

Baker²,

Whitaker

et al. 2022

WMJ

View full text Add to dashboard Cite

Maize is consumed world-wide as staple food, livestock feed, and industrial raw material. However, it is susceptible to fungal attack and at risk of aflatoxin contamination under certain conditions. Such contamination is a serious threat to human and animal health. Ensuring that the maize used by food industry meets standards for aflatoxin levels requires significant investment across the supply chain. Good Agricultural Practices (GAP) form a critical part of a broader, integrated strategy for reduction of aflatoxin contamination. We reviewed and summarised the GAP of maize that would be effective and practicable for aflatoxin control within high-risk regions for smallholder farmers. The suggested practicable GAP for smallholder farmers were: use of drought-tolerant varieties; timely harvesting before physiological maturity; sorting to remove damaged ears and those having poor husk covering; drying properly to 13% moisture content; storage in suitable conditions to keep the crop clean and under condition with minimally proper aeration, or ideally under hermetic conditions. This information is intended to provide guidance for maize growers that will help reduce aflatoxin in high-risk regions, with a specific focus on smallholder farmers. Following the proposed guidelines would contribute to the reduction of aflatoxin contamination during pre-harvest, harvest, and post-harvest stages of the maize value chain.

show abstract

Sustainability of strategies for Ostrinia nubilalis management in Northern Italy: Potential impact on beneficial arthropods and aflatoxin contamination in years with different meteorological conditions

Cited by 10 publications

References 44 publications

Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts

Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts

Validation and Ecological Niche Investigation of a New Fungal Intraspecific Competitor as a Biocontrol Agent for the Sustainable Containment of Aflatoxins on Maize Fields

Review of good agricultural practices for smallholder maize farmers to minimise aflatoxin contamination

Contact Info

Product

Resources

About