Suspension culture of sugarbeet (beta vulgaris L.). induction and habituation of dedifferentiated and self-regenerating cell lines

Geyt, J. P. C. van; Jacobs, M.

doi:10.1007/bf00269208

Cited by 32 publications

(5 citation statements)

References 9 publications

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“…Hormone free growth of root or shoot organ cultures was not attempted. Other work has shown the ability of sugar beet cells (callus and suspension) to habituate to hormone-free medium by successively lowering the auxin concentration, however the ability to grow without exogenous cytokinins appeared to be spontaneous in most of these cultures (Van Geyt and Jacobs, 1985). Doubling times of these cell cultures ranged from 4 to 8 days, similar to that of the rye root cultures reported here.…”

Section: Discussionsupporting

confidence: 59%

Hormone independent root organ cultures of rye (Secale cereale)

Whitney

1996

Plant Cell Tiss Organ Cult

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This work describes the growth of rye root organ cultures which were capable of being repeatedly subcultured in hormone-free medium. They showed morphological characteristics, growth rate, inability to produce shoots, and response to auxins and cytokinins similar to those of the Agrobacterium rhizogenes (Ri plasmid) transformed hairy root cultures of tobacco and red beet which were used for comparison. The root cultures of rye were initiated from callus produced on a medium containing the growth regulators (plant hormones) 2,4-D and kinetin, then transferred to hormone-free medium. However not all rye explants gave rise to callus that would differentiate into stable hairy root cultures and rye seedling root explants did not grow if placed directly on a hormone-free medium. Rice and wheat produced callus and roots on a medium containing hormones but root organ cultures could not be maintained on a hormone-free medium.

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Section: Discussionsupporting

confidence: 59%

Hormone independent root organ cultures of rye (Secale cereale)

Whitney

1996

Plant Cell Tiss Organ Cult

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“…It has been shown that during long-term culture, plant cells may lose the need for auxin and/or cytokinin to maintain active growth. This process known as 'habituation', which is common in callus cultures in some plant species such as sugarbeet [ 50 ], was described as a shift from auxo- to autotrophic state for growth regulator requirements [ 51 ]. Coffee embryogenic cultures do not require auxin or cytokinin to proliferate, although it was possible to stimulate proliferation by optimum exogenous concentrations of both growth regulators.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

et al. 2011

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BackgroundFollowing genome sequencing of crop plants, one of the main challenges today is determining the function of all the predicted genes. When gene validation approaches are used for woody species, the main obstacle is the low recovery rate of transgenic plants from elite or commercial cultivars. Embryogenic calli have frequently been the target tissue for transformation, but the difficulty in producing or maintaining embryogenic tissues is one of the main problems encountered in genetic transformation of many woody plants, including Coffea arabica.ResultsWe identified the conditions required for successful long-term proliferation of embryogenic cultures in C. arabica and designed a highly efficient and reliable Agrobacterium tumefaciens-mediated transformation method based on these conditions. The transformation protocol with LBA1119 harboring pBin 35S GFP was established by evaluating the effect of different parameters on transformation efficiency by GFP detection. Using embryogenic callus cultures, co-cultivation with LBA1119 OD600 = 0.6 for five days at 20 °C enabled reproducible transformation. The maintenance conditions for the embryogenic callus cultures, particularly a high auxin to cytokinin ratio, the age of the culture (optimum for 7-10 months of proliferation) and the use of a yellow callus phenotype, were the most important factors for achieving highly efficient transformation (> 90%). At the histological level, successful transformation was related to the number of proembryogenic masses present. All the selected plants were proved to be transformed by PCR and Southern blot hybridization.ConclusionMost progress in increasing transformation efficiency in coffee has been achieved by optimizing the production conditions of embryogenic cultures used as target tissues for transformation. This is the first time that a strong positive effect of the age of the culture on transformation efficiency was demonstrated. Our results make Agrobacterium-mediated transformation of embryogenic cultures a viable and useful tool both for coffee breeding and for the functional analysis of agronomically important genes.

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“…Habituation or autonomy for one or several exogenous growth regulators characterizes some callus types which is not without relation with their anatomical structure [9]. Callus anatomical and biochemical characteristics certainly can be affected by the culture media and the environmental conditions since they can be changed by chemical and physical manipulations [8,38]. There are, however, some indications that calli may continue to express biochemical traits of the mother plants [14,22,36,40].…”

Section: Introductionmentioning

confidence: 99%

Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

Bourgeade

Boyer

Jaegher

et al. 1989

Plant Cell Tiss Organ Cult

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Calli have been initiated in vitro from young internodes (control and rubbed) of Bryonia dioica, where previously it had been shown, using intact plants, that rubbing induced limited growth through enhanced lignification. Calli derived from rubbed internodes were somewhat more compact and showed biochemical changes, i.e. enhanced activity of total peroxidase and isoperoxidases, enhanced production of l-aminocyclopropane-l-carboxylic acid (ACC) and ethylene, enhanced tissue capacity to convert ACC into ethylene, enhanced activity of phenylalanine ammonia-lyase (PAL) and higher content of lignin, which characterized rubbed internodes. Differences in ethylene metabolism between the two types of calli tended to fade from the third week onwards of initial culture, whereas lignin content, peroxidase activity and peroxidase isoenzyme pattern appeared to be more persistant rubbinginduced markers for several subcultures. The results point to the persistance of environmentally induced changes in gene expression.

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Suspension culture of sugarbeet (beta vulgaris L.). induction and habituation of dedifferentiated and self-regenerating cell lines

Cited by 32 publications

References 9 publications

Hormone independent root organ cultures of rye (Secale cereale)

Hormone independent root organ cultures of rye (Secale cereale)

Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures

Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

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