Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation

Fujii, Genichiro; Kurashina, Yuta; Terao, Yosuke; Azuma, Toshifumi; Morikawa, Akira; Kodeki, Kazuhide; Takahara, Osamu; Takemura, Kenjiro

doi:10.1016/j.ultsonch.2021.105488

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…Recently, Vero cells have been successfully adapted to grow in suspension in serum-free media [ 15 , 16 ]. Fujii et al [ 17 ] fabricated an ultrasonic irradiation system and used CHO cells as a model example, and they found the culture time was reduced by 14% when the cell number increased 1000-fold.…”

Section: Introductionmentioning

confidence: 99%

Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Luo

Niu

et al. 2021

Vaccines

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Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production.

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Section: Introductionmentioning

confidence: 99%

Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Luo

Niu

et al. 2021

Vaccines

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“…Because the principal use of microfluidics technologies is in the improvement of healthcare diagnostics and treatment, many of the applications of acoustofluidics have likewise been in this discipline (Rufo et al, 2022). The vast majority of particle manipulation work, for example, has ultimately been pursued due to a desire to pattern, sort (Franke et al, 2010;Zhang et al, 2021c), propel (Bo et al, 2009Fujii et al, 2021), or agglomerate cells in wells (Kurashina et al, 2017) or open dishware (Mei et al, 2022). Exosome trapping has been an important topic of research via acoustofluidics (Habibi et al, 2020), as exosomes may be produced by cancer cells as they metastasize and may contain genetic information sufficient to identify their source.…”

Section: Biomedicine and Acoustofluidicsmentioning

confidence: 99%

Acoustofluidics

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2023

Front. Acoust.

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The propagation of acoustic waves in fluids and solids produces fascinating phenomena that have been studied since the late 1700s and through to today, where it is finding broad application in manipulating fluids and particles at the micro to nano-scale. Due to the recent and rapid increase in application frequencies and reduction in the scale of devices to serve this new need, discrepancies between theory and reality have driven new discoveries in physics that are underpinning the burgeoning discipline. While many researchers are continuing to explore the use of acoustic waves in microfluidics, some are exploring vastly smaller scales, to nanofluidics and beyond. Because many of the applications incorporate biological material—organelles, cells, tissue, and organs—substantial effort is also being invested in understanding how ultrasound interacts with these materials. Surprisingly, there is ample evidence that ultrasound can be used to directly drive cellular responses, producing a new research direction beyond the established efforts in patterning and agglomerating cells to produce tissue. We consider all these aspects in this mini-review after a brief introduction to acoustofluidics as an emerging research discipline.

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Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation

Abstract: Highlights Exposure to 158 kHz ultrasound enables suspension culture without damaging cells. Cell proliferation was 40% higher than that with the conventional method after 72 h. This technique accelerates the transition to large-scale suspension culture.

Cited by 2 publications

References 31 publications

Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Acoustofluidics

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