Suspected periodontopathogens in erupting third molar sites of periodontally healthy individuals

Abstract

29 periodontally healthy subjects (11 female and 18 male) with a mean age of 24 years (range 19 to 38 years) and with partially erupted lower third molars participated in this study. 18 subjects demonstrated no signs or symptoms of acute inflammation and were without pain (group A). 5 subjects showed redness of the pericoronal tissues and experienced pain upon palpation (group B). 6 subjects suffered from acute pain and exhibited formation of pus (group C). Microbiological samples were taken from the lateral aspect of the pericoronal space using the paperpoint-method. Continuous anaerobic techniques were utilized for microbiological processing. The samples were cultivated on ETSA and on selective media and were studied by darkfield microscopy. Gram-negative anaerobic rods accounted for 27% (group A), 34% (group B) and 39% (group C) of all organisms growing on ETSA. Bacteroides intermedius was detected in 61% (group A), 80% (group B) and 83% (group C) of the samples. B. gingivalis was found in 1 sample of group A only. Fusobacterium sp. was detected in 56% (group A), 80% (group B) and 33% (group C) of the samples. Capnocytophaga were seen in 67% (group A), 20% (group B) and 50% of the samples. Actinobacillus actinomycetemcomitans was found in 44% (group A), 40% (group B) and 17% (group C). 72% of the group A and 100% of the group B and C samples contained spirochetes. In all of those positive samples, small spirochetes were present, but only 78% contained medium and only 48% large spirochetes.(ABSTRACT TRUNCATED AT 250 WORDS)

Periodontal pathogens in erupting third molars of periodontally healthy subjects

“…Bacterial samples were taken from the pericoronal pockets of partly erupted mandibular third molars using the paper-point method 19,24 . In brief, gingival tissue at the sampling site was cleaned with swabs containing 70% ethanol.…”

“…Predominant microbial species of pericoronitis are Streptococcus, Actinomyces and Propionibacterium spp, as well as betalactamase-producing bacteria such as Prevotella, Bacteroides, Fusobacterium, Capnocytophaga and Staphylococcus spp 7,26 , but the principal periodontal pathogens have rarely been isolated in pericoronitis by culturing. Porphyromonas gingivalis has been detected in 100% of samples by LEUNG et al 13 , in 9.1% of samples by RAJASUO et al 24 , and in 20% of healthy third-molar pericoronal pockets by MOMBELLI et al 19 . Actinobacillus actinomycetemcomitans was detected in 17-40% by MOMBELLI et al 19 , in 9.1% by RAJASUO et al 24 significant odds ratio of 1.5 for total coronary heart disease and 1.9 for fatal heart disease.…”

“…Porphyromonas gingivalis has been detected in 100% of samples by LEUNG et al 13 , in 9.1% of samples by RAJASUO et al 24 , and in 20% of healthy third-molar pericoronal pockets by MOMBELLI et al 19 . Actinobacillus actinomycetemcomitans was detected in 17-40% by MOMBELLI et al 19 , in 9.1% by RAJASUO et al 24 significant odds ratio of 1.5 for total coronary heart disease and 1.9 for fatal heart disease. MEURMAN et al 17 reviewed all the published evidence up to the year 2004 regarding the relationships between oral health parameters and atherosclerosis and cardiovascular diseases.…”

Periodontal bacteria in different sampling sites of pericoronitis patients

“…Porphyromonas gingivalis (P.g.) has been detected in 100% of the samples of Leung et al [3], but only in 9.1% of the samples of Rajasuo et al [4] and in 20% of healthy third-molar pericoronal pockets by Mombelli et al [5]. Aggregatibacter actinomycetemcomitans (A.a.) has been detected in 9.1% [4], in 17% -40% [5] and in 10.8% [6] of the samples, respectively.…”

“…A 0.8-mm-thick sterile endodontic paper-point was then inserted into the depth of the pericoronal and gingival pockets, respectively, and left in place for 30 seconds as described by Mombelli et al [5] and Rajasuo et al [4]. Saliva samples were collected from the bottom of the mouth using a sterile syringe, and tongue samples by scraping the dorsum of the tongue with a sterile metal spatula.…”