Suspect screening of pharmaceuticals in fish livers based on QuEChERS extraction coupled with high resolution mass spectrometry

Baesu, Anca; Ballash, Gregory A.; Mollenkopf, Dixie F.; Wittum, Thomas E.; Sullivan, Sean; Bayen, Stéphane

doi:10.1016/j.scitotenv.2021.146902

Cited by 14 publications

(13 citation statements)

References 61 publications

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“…In most of the literature consulted, specimens were captured by professional divers in different sampling areas, although in some cases, they were purchased in local supermarkets, either to be used as an analyte-free matrix [ 53 ], or as study sample [ 64 , 74 , 75 ]. Once captured, they were transported on ice, in order to avoid decomposition, at −10 °C and stored frozen at −20 °C [ 53 , 72 , 77 ], or deep-frozen (around −80 °C) until analysis [ 64 , 73 , 78 ].…”

Section: Analytical Methodologies For the Determination Of Pharmaceut...mentioning

confidence: 99%

“…López-García et al [ 61 ] used ACN with salts (MgSO 4 , NaCl, sodium citrate and DCS (sodium citrate sesquihydrate)) for the study of mussel’s tissue, with recoveries between 77% and 118% and and low LODs (<2 ng g −1 ). Bobrowska-Korczak et al [ 64 ] and Miossec et al [ 73 ], studied the presence of 98 and 41 PhACs, respectively, in fish and shrimps, with LODs between 0.1 and 40.2 ng g −1 , reaching recoveries in the range of 28 to 188%.…”

Section: Analytical Methodologies For the Determination Of Pharmaceut...mentioning

confidence: 99%

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An Overview of Analytical Methods to Determine Pharmaceutical Active Compounds in Aquatic Organisms

et al. 2022

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There is increasing scientific evidence that some pharmaceuticals are present in the marine ecosystems at concentrations that may cause adverse effects on the organisms that inhabit them. At present, there is still very little scientific literature on the (bio)accumulation of these compounds in different species, let alone on the relationship between the presence of these compounds and the adverse effects they produce. However, attempts have been made to optimize and validate analytical methods for the determination of residues of pharmaceuticals in marine biota by studying the stages of sample treatment, sample clean-up and subsequent analysis. The proposed bibliographic review includes a summary of the most commonly techniques, and its analytical features, proposed to determine pharmaceutical compounds in aquatic organisms at different levels of the trophic chain in the last 10 years.

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Section: Analytical Methodologies For the Determination Of Pharmaceut...mentioning

confidence: 99%

Section: Analytical Methodologies For the Determination Of Pharmaceut...mentioning

confidence: 99%

An Overview of Analytical Methods to Determine Pharmaceutical Active Compounds in Aquatic Organisms

et al. 2022

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“…Frozen liver samples were homogenized, and extractions were conducted on 0.5 g of sample as previously described [16]. In brief, a 100 μL sample of extract was diluted to 1 mL with water and analyzed using an Agilent UHPLC 1290 coupled with an Agilent 6545 QTOF-ESI-MS in both positive and negative ionization modes.…”

Section: Target and Suspect Screening Of Antimicrobial Residuesmentioning

confidence: 99%

“…In addition, sample extracts were screened for suspect antimicrobial targets using the Agilent Mass Hunter Profinder software B.010.0 and importing profinder archive files (.pfa) into Mass Profiler Professional (v 15.0 Agilent Technologies) for searches among the Agilent Veterinary Drug PCDL (2153 compounds) and Water Screening PCDL (1451 compounds) libraries. The method was evaluated earlier based on recovery, precision and matrix effect, with different approaches, e.g., quality control samples, taken to assess the quality of the data [16]. PLOS ONE…”

Section: Target and Suspect Screening Of Antimicrobial Residuesmentioning

confidence: 99%

Fish as sentinels of antimicrobial resistant bacteria, epidemic carbapenemase genes, and antibiotics in surface water

et al. 2022

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Surface waters, especially those receiving wastewater flows, can disseminate antimicrobial resistant bacteria (ARB), antimicrobial resistance genes (ARG), and antibiotics. In the Scioto River of central Ohio, United States, we evaluated fishes as potential sentinels of ARB and antimicrobial contamination and investigated the influence of antimicrobial exposure on the fish intestinal resistome. Seventy-seven fish were collected from river reaches receiving inputs from two wastewater treatment plants that serve the greater Columbus Metropolitan Area. Fish were screened for the presence of cephalosporin-resistant (CeRO) and carbapenem-resistant (CRO) organisms, epidemic carbapenemase genes, and antibiotic drugs and metabolites using culture methods, droplet digital PCR, and ultra-high performance liquid chromatography tandem mass spectroscopy (UHPLC-MS/MS). Nearly 21% of fish harbored a CeRO in their resistome, with 19.4% exhibiting bacteria expressing an AmpC genotype encoded by blaCMY, and 7.7% with bacteria expressing an extended-spectrum β-lactamase phenotype encoded by blaCTX-M. blaKPC and blaNDM were present in 87.7% (57/65) and 80.4% (37/46) of the intestinal samples at an average abundance of 104 copies. Three antibiotics–lincomycin (19.5%), azithromycin (31.2%) and sulfamethoxazole (3.9%)–were found in hepatic samples at average concentrations between 25–31 ng/g. Fish harboring blaCTX-M and those exposed to azithromycin were at greater odds of being downstream of a wastewater treatment plant. Fish that bioconcentrated antibiotics in their liver were not at greater odds of harboring CeRO, CRO, or epidemic carbapenemase gene copies in their resistome. Our findings confirm that fishes can be effective bioindicators of surface waters contaminated with ARB, ARG, and antibiotics. Moreover, our findings highlight the varying importance of different mechanisms that facilitate establishment of ARB in aquatic ecosystems.

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“…Among these, PSA and C 18 can effectively remove polar compounds such as fatty acids, organic acids, sugars, and polar pigments, as well as weakly polar compounds such as oils, proteins, fat-soluble vitamins, and fats. Yet, PSA and C 18 have the disadvantages of poor adsorption stability due to the influence of pH, temperature, and other factors 23 , large usage, and high cost 24 . GCB has a planar hexagonal structure similar to graphite, which is strongly adsorbed on pesticide compounds with planar structure, thus affecting the recovery rate of pesticides with planar structure 25 .…”

Section: Introductionmentioning

confidence: 99%

Residual levels and dietary intake risk assessment of 11 pesticides in apricots from different ecological planting regions in China

Yang

Xing

Liu

et al. 2022

Sci Rep

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The frequent and massive use of pesticides has led to pesticide residues in apricot, threatening food safety and human health. A reliable and simple modified QuEChERS method with ultra-performance liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of 11 pesticides in apricot. Method validation indicated that satisfied linearity (R2 ≥ 0.9959), accuracy (recoveries of 72–119%), sensitivity (limits of detection, 0.03–0.30 μg/kg; limits of quantification, 0.13–1.00 μg/kg), and precision (relative standard deviations ≤ 11.9%), and matrix effects were 0.89–1.13. Apricot samples from different ecological regions in China were collected and tested using the proposed methods. Monitoring results were used to assess the dietary intake risk of Chinese populations of different ages and genders. Dietary risk assessment revealed that the risk quotients were 0.003–1.184% for different gender and age groups in China, indicating none unacceptable public health risk for general population. This work was thus significant in developing a simpler, more efficient and economical analysis method and food safety risks of the 11 pesticides on apricot and facilitated the establishment of maximum residue limits.

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Suspect screening of pharmaceuticals in fish livers based on QuEChERS extraction coupled with high resolution mass spectrometry

Cited by 14 publications

References 61 publications

An Overview of Analytical Methods to Determine Pharmaceutical Active Compounds in Aquatic Organisms

An Overview of Analytical Methods to Determine Pharmaceutical Active Compounds in Aquatic Organisms

Fish as sentinels of antimicrobial resistant bacteria, epidemic carbapenemase genes, and antibiotics in surface water

Residual levels and dietary intake risk assessment of 11 pesticides in apricots from different ecological planting regions in China

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