Suscetibilidade a desinfetantes e perfil de resistência a antimicrobianos em isolados de Escherichia coli

Abstract

RESUMO.-A colibacilose, causada por Escherichia coli, é a enfermidade entérica de maior impacto na produção de suínos, podendo levar à morte do animal. Esta bactéria possui grande capacidade de desenvolver resistência a múltiplos antimicrobianos e a desinfetantes. Desta forma, estudos que abordem mecanismos de resistência e perϐil de amostras de campo tornam-se necessários. E. coli é amplamente utilizada como modelo de estudos que exploram a resistência intrín-seca e extrínseca a multidrogas. Neste trabalho, buscou-se Colibacillosis caused by Escherichia coli is the most important enteric disease in pig production, which may lead to death of the affected animal. The bacterium has a great ability to develop resistance to multiple antibiotics and disinfectants. Thus, investigation addressing mechanisms of resistance and proϐile of ϐield samples is necessary. E. coli is widely used as a model for studies that explore the intrinsic and extrinsic resistance to multidrugs. In this paper, we attempt to associate the susceptibility proϐile of 62 isolates of E. coli to three disinfectants and 13 antimicrobials. Also 31 isolates were tested for the presence of efϐlux mechanism. Of the three disinfectants tested, alkyldimethylbenzylammonium chloride+nonyl phenoxy polyethoxy ethanol was the most effective (100%), followed by glutaraldehyde+al kyldimethylbenzylammonium chloride (95.2%) and alkyldimethylbenzylammonium chloride (88.8%). Among the antimicrobials tested, there was greater resistance to tetracycline (62.2%) and higher sensitivity to ϐlorfenicol (88.6%). The high sensitivity of the isolates against disinfectants may be related to the absence of efϐlux mechanism. The average index of multiple resistance to antimicrobials was 0.52, what demonstrates a proϐile of multidrug resistant isolates, showing the need for rational use of these drugs in pig production.

Phylogenetic analysis, biofilm production, and antimicrobial resistance profile of Escherichia coli isolated from slaughtered pigs

“…This was observed in the present study, in which 99.21% (125/126) of the strains exhibited multiresistance, which is a similar result to that obtained by Krewer et al (2012), who reported that this result could indicate the potential for horizontal transfer of resistance genes. Mota et al (2005) reported that high rates of multiple resistance to antimicrobials represent a great risk to public health by hindering the treatment of animal and human diseases.…”

“…Baccaro et al (2002) recorded 97% of strains resistant to this antimicrobial when analyzing 600 E. coli isolates from piglets with diarrhea in the state of São Paulo. Ten years later, Krewer et al (2012) analyzed 62 isolates of E. coli from the feces of pigs in the south of Brazil and found that 16.3% of the samples were resistant to this active ingredient. This behavior could be explained by a determination from the Ministry of Agriculture, Fishing and Supplies (Normative Instruction no.…”

Caracterização Físico-Química, Antioxidante E De Aminoácidos Da Castanha Do Baru, Castanha De Caju E Castanha-Do-Brasil

“…Muitos mecanismos podem ser utilizados pela bactéria para adquirir resistência aos antimicrobianos, dentre eles temos a chamada resistência adquirida, que é a expressão de bombas de efluxo que expulsam moléculas do antibiótico do meio intracelular para o extracelular, impedindo ou dificultando sua ligação com o sitioalvo, causando, desta maneira, diminuição ou supressão da sensibilidade a múltiplos antimicrobianos ou podem ocorrer mutações em um ou mais genes que codificam regiões-alvo (proteínas) destes antimicrobianos (BAPTISTA, 2013;KREWER et. al., 2012;KAUR, 2018).…”

Evaluation of homeopathic treatment of Escherichia coli infected swine with indetification of virulence factors involved: Pilot study

“…Enteritis can appear in three different stages: neonatal diarrhea appears in the first days of life; piglet diarrhea from the first week of life to weaning; and post-weaning diarrhea. E. coli is the agent most frequently associated with neonatal and post-weaning diarrhea in swine [14,15,16,17,18,19,20,21,22]. From the clinical point of view, piglets might refuse to suckle and exhibit watery diarrhea leading quickly to dehydration, weakness and eventually death [15,22,23].…”