Susceptibility to Ulcerative Colitis and Genetic Polymorphisms of A251G SOD1 and C-262T CAT

Abstract

SummaryBackgroundReactive oxygen species can attack and damage almost every molecule found in living cells, including proteins, carbohydrates, lipids, and DNA. For this reason, their production is normally tightly controlled. Among the most important defenses against these radicals are the superoxide dismutase (SOD) enzymes and catalase (CAT). Increasing attention has been given to the role of reactive oxygen metabolites in the pathogenesis of ulcerative colitis (UC), which is defined as an idiopathic and chronic intestinal inflammation. Accordingly, we hypothesized a relation between genetic polymorphisms in the two antioxidant enzymes SOD1 A251G (rs2070424) and CAT C-262T (rs1001179) and the risk of UC.MethodsThe present case-control study included 109 UC patients (46 males and 50 females) and 186 (67 males and 119 females) gender-matched healthy controls. Genotyping was done by the PCR-RFLP method.ResultsAfter adjusting for age and gender, a significant association was observed between the AG+GG genotypes of SOD1 A251G polymorphism (vs. AA genotype) and risk of UC (OR=0.29, 95% CI: 0.10–0.86, P= 0.025) after adjusting for age and gender. Our statistical analysis revealed that the CAT C-262T polymorphism did not associate with the risk of UC before and/or after adjusting for age and gender.ConclusionsBased on the present statistical analysis, the G allele of the SOD1 A251G polymorphism decreases the risk of UC, thus it might be assumed that the G allele has a protective role.

The copper‐zinc superoxide dismutase activity in selected diseases

“…Individuals with AA genotype were characterized by the highest serum SOD activity, compared to patients with genotypes: AG and GG . Interestingly, a polymorphism of SOD1 has recently been associated with IBD, as it has been claimed, using PCR‐RFLP method, that the G allele of the SOD1 rs2070424 (A251G) decreases the risk of UC . The association of SOD1 and SOD3 polymorphisms with the risk of IBD development needs further analysis.…”

Downregulation of Dickkopf-1 Augments the Therapeutic Effects of Endometrial Regenerative Cells on Experimental Colitis

“…IL-10 partcipates in suppressing the antigen presentations and the synthesis of pro-in ammatory cytokines in colitis [60]. SOD, is a critical antioxidant enzyme, which could catalyze the dismutation of toxic superoxides and scavenge superoxide radicals in colons [44]. On the contrary, IFN-γ, TNF-α, MPO, COX-2 and iNOs are pro-in ammatory factors which often strongly exacerbate the in ammations cascade in colitis [61][62][63].…”

“…Meanwhile, we also tested the SOD (superoxide dismutases) mRNA expression in different groups ( Figure 5K). SOD, which is regarded as a critical antioxidant enzyme, could catalyze the dismutation of toxic superoxides and scavenge superoxide radicals in colitis [44]. In our present study, we found that SOD mRNA expressions were signi cantly raised in DKK1 low -ERC group (DKK1 low -ERC group vs. unmodi ed ERC group, p < 0.01), but plummeted in DKK1 high -ERC group (DKK1 high -ERC group vs. unmodi ed ERC group, SOD, p < 0.01).…”

Superoxide dismutase coding of gene polymorphisms associated with susceptibility to Parkinson's disease

“…However, genes for these three SODs (SOD1, SOD2, SOD3) are highly polymorphic in human, with certain polymorphisms reported to be associated with serious diseases (Kim et al, 2015;Xu et al, 2012). For example, SOD1 A/G (rs2070424) polymorphism may decrease the risk of (and G allele might protect from) ulcera- tive colitis (El-Kheshen et al, 2016) and gastric cancer (Ebrahimpour and Saadat, 2014). Further, SOD2 T/C (rs4880) is reported to be associated with noise-induced hearing loss in humans (Liu et al, 2010), while SOD3 C/G (rs1799895) is associated with cardiovascular disease (Naganuma et al, 2008) and preeclampsia (Procopciuc et al, 2012).…”