Susceptibility to sulfuryl fluoride and lack of cross-resistance to phosphine in developmental stages of the red flour beetle,<i>Tribolium castaneum</i>(Coleoptera: Tenebrionidae)

Jagadeesan, Rajeswaran; Nayak, Manoj K.; Pavic, Hervoika; Chandra, Kerri A.; Collins, Patrick J.

doi:10.1002/ps.3940

Cited by 44 publications

(27 citation statements)

References 40 publications

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“…Mortality response in eggs, larvae and pupae was estimated based on the procedure described previously by Jagadeesan et al (2015) in which the number of live adults that emerged from control jars was compared with the number of live adults that emerged in treated jars for each of the respective life stages. These mortality values were corrected using Abbott's formula (Abbott 1925) and subjected to probit analysis (Finney 1979) factors.…”

Section: Discussionmentioning

confidence: 99%

Relative tolerance and expression of resistance to phosphine in life stages of the rusty grain beetle, Cryptolestes ferrugineus

et al. 2017

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Cryptolestes ferrugineus is a serious cosmopolitan pest of stored products. Frequent and indiscriminate usage of phosphine has caused the development of high levels of resistance to this fumigant. As there are few alternatives, it is imperative that resistance to phosphine is managed. Effective management requires knowledge of key factors driving the rate of selection. One of the most important factors is the response of each resistance genotype to phosphine, especially heterozygotes. Moreover, it is important to understand the expression of resistance in all life stages as all stages are subjected to selection during fumigation. We determined the relative tolerance and resistance levels to phosphine in all life stages of homozygous parental strains (susceptible and resistant) and their F 1 progeny (heterozygous) and estimated relative dominance of resistance within life stages over 48 h. In susceptible insects, relative tolerance was highest in eggs followed by pupae, then adults which had about the same tolerance as larvae. In homozygous resistant insects, the order of tolerance was adult = egg [ pupae [ larvae and in heterozygotes larvae [ eggs [ pupae [ adults. All life stages expressed resistance with resistance ratios highest in adults [ pupae [ larvae [ eggs. At LC 50 , resistance was incompletely recessive in eggs, pupae and adults and incompletely dominant in larvae. Eggs and adults were also incompletely recessive at LC 95 , but larvae were completely dominant and pupae were incompletely dominant. Our data showed that a proportion of heterozygotes in all life stages, the major carriers of resistance in the field, will survive at very high concentrations, particularly in the egg stage, forming a nucleus for reinfestation or dispersal of resistance.

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Section: Discussionmentioning

confidence: 99%

Relative tolerance and expression of resistance to phosphine in life stages of the rusty grain beetle, Cryptolestes ferrugineus

et al. 2017

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“…However, frequent and widespread use of chemical fumigants has led to the development of resistance in stored-product insects [5]. Furthermore, due to the destruction of earth’s ozone layer, residue formation, and carcinogenicity, some chemical fumigants have been prohibited [6, 7]. Therefore, it is critical to search for novel fumigants for combating stored-product insects.…”

Section: Introductionmentioning

confidence: 99%

Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation

et al. 2016

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BackgroundThe cereal weevil, Sitophilus zeamais is one of the most destructive pests of stored cereals worldwide. Frequent use of fumigants for managing stored-product insects has led to the development of resistance in insects. Essential oils from aromatic plants including the tea oil plant, Melaleuca alternifolia may provide environmentally friendly alternatives to currently used pest control agents. However, little is known about molecular events involved in stored-product insects in response to plant essential oil fumigation.ResultsM. alternifolia essential oil was shown to possess the fumigant toxicity against S. zeamais. The constituent, terpinen-4-ol was the most effective compound for fumigant toxicity. M. alternifolia essential oil significantly inhibited the activity of three enzymes in S. zeamais, including two detoxifying enzymes, glutathione S-transferase (GST), and carboxylesterase (CarE), as well as a nerve conduction enzyme, acetylcholinesterase (AChE). Comparative transcriptome analysis of S. zeamais through RNA-Seq identified a total of 3,562 differentially expressed genes (DEGs), of which 2,836 and 726 were up-regulated and down-regulated in response to M. alternifolia essential oil fumigation, respectively. Based on gene ontology (GO) analysis, the majority of DEGs were involved in insecticide detoxification and mitochondrial function. Furthermore, an abundance of DEGs mapped into the metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were associated with respiration and metabolism of xenobiotics, including cytochrome P450s, CarEs, GSTs, and ATP-binding cassette transporters (ABC transporters). Some DEGs mapped into the proteasome and phagosome pathway were found to be significantly enriched. These results led us to propose a model of insecticide action that M. alternifolia essential oil likely directly affects the hydrogen carrier to block the electron flow and interfere energy synthesis in mitochondrial respiratory chain.ConclusionThis is the first study to perform a comparative transcriptome analysis of S. zeamais in response to M. alternifolia essential oil fumigation. Our results provide new insights into the insecticidal mechanism of M. alternifolia essential oil fumigation against S. zeamais and eventually contribute to the management of this important agricultural pest.

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“…fumigant with a different mode of action can effectively be used to curtail the selection of resistant individuals of C. ferrugineus and other grain insect species [40]. In addition, the results of this work will assist with identification of the rph1 gene, which may provide new control opportunities to manage resistance to phosphine in grain insect pests.…”

Section: Accepted M Manuscriptmentioning

confidence: 98%

Genetic characterization of field-evolved resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Laemophloeidae: Coleoptera)

Jagadeesan

Collins

Nayak

et al. 2016

Pesticide Biochemistry and Physiology

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Inheritance of resistance to phosphine fumigant was investigated in three field-collected strains of rusty grain beetle, Cryptolestes ferrugineus, Susceptible (S-strain), Weakly Resistant (Weak-R) and Strongly Resistant (Strong-R). The strains were purified for susceptibility, weak resistance and strong resistance to phosphine, respectively, to ensure homozygosity of resistance genotype. Crosses were established between S-strain x Weak-R, S-strain x Strong-R and Weak-R x Strong-R, and the dose mortality responses to phosphine of these strains and their F 1 , F 2 and F 1 -backcross progeny were obtained. The fumigations were undertaken at 25°C and 55% RH for 72 hours.Weak-R and Strong-R showed resistance factors of 6.3× and 505× compared with S-strain at the LC 50 .Both weak and strong resistances were expressed as incompletely recessive with degrees of dominance of -0.48 and -0.43 at the LC 50 , respectively. Responses of F 2 and F 1 -backcross progeny indicated the existence of one major gene in Weak-R, and at least two major genes in Strong-R, one of which was allelic with the major factor in Weak-R. Phenotypic variance analyses also estimated that the number of independently segregating genes conferring weak resistance was 1 (nE = 0.89) whereas there were two genes controlling strong resistance (nE = 1.2). The second gene, unique to Strong-R, interacted synergistically with the first gene to confer a very high level of resistance (~80×).Neither of the two major resistance genes was sex linked. Despite the similarity of the genetics of resistance to that previously observed in other pest species, a significant proportion (~15 to 30%) of F 1 individuals survived at phosphine concentrations higher than predicted. Thus it is likely that additional dominant heritable factors, present in some individuals in the population, also influenced the resistance phenotype.Our results will help in understanding the process of selection for phosphine resistance in the field which will inform resistance management strategies. In addition, this information will provide a basis for the identification of the resistance genes.

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Susceptibility to sulfuryl fluoride and lack of cross-resistance to phosphine in developmental stages of the red flour beetle,Tribolium castaneum(Coleoptera: Tenebrionidae)

Cited by 44 publications

References 40 publications

Relative tolerance and expression of resistance to phosphine in life stages of the rusty grain beetle, Cryptolestes ferrugineus

Relative tolerance and expression of resistance to phosphine in life stages of the rusty grain beetle, Cryptolestes ferrugineus

Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation

Genetic characterization of field-evolved resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Laemophloeidae: Coleoptera)

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