Susceptibility to Coolness at the Young Microspore Stage under High Nitrogen Supply in Rice (<i>Oryza Sativa</i>L.). Proteome Analysis of Mature Anthers

Hayashi, Takami; Yamaguchi, Tomoya; Nakayama, Katsuhiro; Komatsu, Setsuko; Koike, Setsuo

doi:10.1626/pps.9.212

Cited by 14 publications

(21 citation statements)

References 35 publications

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“…However, transcript-level (mRNA) expression does not always correlate to the quality and quantity of the gene product, i.e., proteins (Abbott 1999;Pandey and Mann 2000;Zivy and de Vienne 2000), because of alternative splicing and post-translational modifications (Komatsu 2005). Hence, 2D protein expression has been extensively employed in a wide range of studies to analyze different stages of pollen development (Imin et al 2001;Kerim et al 2003;Dai et al 2006) and cold stress at the young microspore stage (Hayashi et al 2006;Imin et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Jagadish

Raveendran

Rang

et al. 2011

Rice

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In future climates, rice crops will be frequently exposed to water deficit and heat stress at the most sensitive flowering stage, causing spikelet sterility and yield losses. Water deficit alone and in combination with heat stress significantly reduced peduncle elongation, trapping 32% and 55% of spikelets within the leaf sheath, respectively. Trapped spikelets had lower spikelet fertility (66% in control) than those exserted normally (>93%). Average weighted fertility of exserted spikelets was lowest with heat stress (35%) but higher with combined stress (44%), suggesting acquired thermo-tolerance when preceded by water-deficit stress. Proteins favoring pollen germination, i.e., pollen allergens and beta expansin, were highly upregulated with water deficit but were at normal levels under combined stress. The chaperonic heat shock transcripts and proteins were significantly up-regulated under combined stress compared with either heat or water deficit. The importance of spikelet proteins responsive to water deficit and heat stress to critical physiological processes during flowering is discussed.

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Section: Introductionmentioning

confidence: 99%

Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Jagadish

Raveendran

Rang

et al. 2011

Rice

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“…Satake (1991) reported that cool temperature causes reduction in the number of microspore and pollen grains during microsporogenesis. The pollen germination ratio was similarly found declined further when nitrogen fertilization was done under the cool weather temperature during flowering (Hayashi et al, 2006) because of declined number of engorged pollen grains. It has been reported that less fertile spikelets were observed in rice grown in cool weather during flowering with high nitrogen fertilization (Sasaki and Wada, 1975;Amano and Moriwaki, 1984;Satake et al, 1987).…”

Section: Discussionmentioning

confidence: 95%

“…But, excessive N fertilization causes reduction in floral potency which was declared through decline in the number of pollen grains (Tatsuta, 1999;Hayashi et al, 2000). The pollen germination ratio was similarly found declined further when high dose of nitrogen fertilization was done under the cool weather temperature during flowering which was indicated from declined number of engorged pollen grains (Hayashi et al, 2006). It has been reported that less fertile spikelets were observed in rice crop grown in cool temperature during flowering with high nitrogen fertilization (Sasaki and Wada, 1975;Amano and Moriwaki, 1984;Satake et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Flowering, reproductive behaviors and their effects on grain yields of newly bred single cross hybrids of yellow maize (Zea mays L.) in winter in subtropical Nepalese Himalayan foot plain

Adhikari

Ghimire

Sah

et al. 2015

Preprint

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Introduction:All the plants of a maize population or hybrid PP (plant population) do not dehisce anthers and do not emerge silk simultaneously. Generally, male flowering or anther dehiscence (anthesis) is leading and female flowering (silk emergence or silking) is lagging under both optimum and stressful environment. Enough and timely pollen availability is indispensable when silks come out of maize ears for largest number of kernel set. So; study of flowering and reproductive behavior of newly bred maize hybrids are indispensable to confirm high grain yielding hybrid with traits of synchrony in anthesis and silking, coming out of all silks and enough pollen availability in short duration. Materials and methods: Flowering and reproductive behaviors of fifteen newly bred single cross hybrids of yellow maize have been examined growing them in an RCBD trial planting their seeds on October 3, 2012 to expose flowering to the natural winter in subtropical foot plain of Himalaya. For flowering behavior; emergence of tassel, anthesis, silking, silk senescence, tassel-anthesis interval, anthesis-silking interval, silking-silk senescence interval of the hybrids have been examined dissecting the PP into four equal parts (percent) as the first earliest, second earliest, third earliest and terminal 25% of the hybrid PP denominated as 25, 50, 75 and 100% respectively of the each of the fifteen hybrids. So, whether third earliest 25% and terminal 25% silk emerging PP of each of the hybrids (SILK75 and SILK100%) respectively received enough pollen from the PP of the same hybrid can be determined through ASI 75 and ASI 100 of the hybrid PP. Results and discussion: Among some polynomial regression equations (PREs) discovered based on floral traits, a grain yield estimating PRE (t ha -1 ) Y = -11922 + 507.3*(TSS25) 1 -7.183*(TSS25) 2 + 0.03387*(TSS25) 3 with r 2 value 66% has been discovered from days taken for TSS25. Similarly, high r 2 bearing PREs were from days for anthesis and silking. PREs with the lowest r 2 were for the days required for the silk senescence (S SEN). Reasons of the low r 2 are: the observation of silk browning or withering could not be precise for naked eye since the silks looked fresh longer in high humidity, wellirrigated state, low temperature, low sun-shine and foggy or cloudy day although the silks lost receptivity earlier in the winter. In addition, interval duration from SILK INI to S SEN cannot be the duration of silk receptivity in the winter of subtropical Nepalese Himalayan foot plain. For reproductive behavior; number of functional egg cells that became successful zygotes to pull assimilates for complete kernel development can reflect aroma about reproductive strength (RS) of the hybrid. Accordingly, hybrids 8 and 5 have been found of the highest RS.

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“…Furthermore, plants exposed to cool temperatures that were grown under High-N show lower fertility than the plants under normal nitrogen even though they have almost the same number of engorged pollen grains on stigma (Hayashi et al, 2000). The pollen germination ratio is also decreased by cooling at the young microspore stage and this decrease is enhanced under High-N conditions (Hayashi et al, 2006). These results suggest that the increase in sterile spikelets due to the cool temperature and High-N is caused by the enhanced decreases in the number of engorged pollen grains and the germination ratio.…”

mentioning

confidence: 98%

“…In the previous report, the proteome analysis of the rice mature anthers was used to examine the changes in proteins under High-N and/or High-N plus cooling at the young microspore stage (High-N-cooling), and changes in 11 proteins involved in cell elongation, stress responses and sugar metabolism were observed (Hayashi et al, 2006). It is noteworthy that 3 out of these 11 proteins are identified as expansin (EXP).…”

mentioning

confidence: 99%

Effects of High Nitrogen Supply on the Susceptibility to Coolness at the Young Microspore Stage in Rice (Oryza sativaL.): Gene Expression Analysis in Mature Anthers

Hayashi

Yamaguchi

Nakayama

et al. 2009

Plant Production Science

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Susceptibility to Coolness at the Young Microspore Stage under High Nitrogen Supply in Rice (Oryza SativaL.). Proteome Analysis of Mature Anthers

Cited by 14 publications

References 35 publications

Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Spikelet Proteomic Response to Combined Water Deficit and Heat Stress in Rice (Oryza sativa cv. N22)

Flowering, reproductive behaviors and their effects on grain yields of newly bred single cross hybrids of yellow maize (Zea mays L.) in winter in subtropical Nepalese Himalayan foot plain

Effects of High Nitrogen Supply on the Susceptibility to Coolness at the Young Microspore Stage in Rice (Oryza sativaL.): Gene Expression Analysis in Mature Anthers

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