Susceptibility to Apoptosis in Insulin-like Growth Factor-I Receptor-deficient Brown Adipocytes

Valverde, Ángela M.; Mur, Cecilia; Brownlee, Michael; Benito, Manuel

doi:10.1091/mbc.e03-11-0853

Cited by 23 publications

(16 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Insulin has been reported to protect various cell types from apoptotic cell death induced by different means such as elevated glucose, cytokines, fatty acids, and serum withdrawal [12,[29][30][31][32][33]. In accord with these studies, we observed that insulin reduced effects of H 2 O 2 to increase LDH release and reduce viability of CHO and HEK293 cells (Bucris, Sampson and Zick, unpublished) and had slight protective effects of its own on these and other (PC12 pheochromocytoma and AML-12 liver cells) nonpancreatic b cells.…”

Section: Discussionmentioning

confidence: 99%

Insulin increases H2O2-induced pancreatic beta cell death

et al. 2010

View full text Add to dashboard Cite

Insulin resistance results, in part, from impaired insulin signaling in insulin target tissues. Consequently, increased levels of insulin are necessary to control plasma glucose levels. The effects of elevated insulin levels on pancreatic beta (β) cell function, however, are unclear. In this study, we investigated the possibility that insulin may influence survival of pancreatic β cells. Studies were conducted on RINm, RINm5F and Min-6 pancreatic β-cells. Cell death was induced by treatment with H(2)O(2), and was estimated by measurements of LDH levels, viability assay (Cell-Titer Blue), propidium iodide staining and FACS analysis, and mitochondrial membrane potential (JC-1). In addition, levels of cleaved caspase-3 and caspase activity were determined. Treatment with H(2)O(2) increased cell death; this effect was increased by simultaneous treatment of cells with insulin. Insulin treatment alone caused a slight increase in cell death. Inhibition of caspase-3 reduced the effect of insulin to increase H(2)O(2)-induced cell death. Insulin increased ROS production by pancreatic β cells and increased the effect of H(2)O(2). These effects were increased by inhibition of IR signaling, indicative of an effect independent of the IR cascade. We conclude that elevated levels of insulin may act to exacerbate cell death induced by H(2)O(2) and, perhaps, other inducers of apoptosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Insulin increases H2O2-induced pancreatic beta cell death

et al. 2010

View full text Add to dashboard Cite

show abstract

“…To determine the potential influence of Fas/FasL interaction on IL-10-aNSC-induced apoptosis of MOG-reactive T cells, splenocytes of naive MOG TCR transgenic mice, of which all CD4 + T cells are MOG35-55 specific (55), were cultured in the presence of MOG35-55 (10 μg/ml) and purified anti-FasL antibody (no azide/low endotoxin, BD Biosciences -Pharmingen) in different concentrations (0, 10, 50, 250 ng/ml) to block FasL (56,57). Four days later, these cells were cocultured with aNSCs at a ratio of 10:1 for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Adult neural stem cells expressing IL-10 confer potent immunomodulation and remyelination in experimental autoimmune encephalitis

Yang¹,

Jiang²,

Fitzgerald³

et al. 2009

J. Clin. Invest.

183

179

View full text Add to dashboard Cite

“…IGF1 induces adipocyte differentiation (Scavo et al, 2004) and stimulates metabolic hormone secretion (Maillard et al, 2011). Highly mitochondrial dense and metabolically active BAT is an IGF-I target tissue and fetal BAT has an especially high number of IGF1 receptors (Lorenzo et al, 1993; Valverde et al, 2004). …”

Section: Factors Influencing Adipogenesis: Current Status Of Knowlmentioning

confidence: 99%

Influence of maternal obesity, diet and exercise on epigenetic regulation of adipocytes

Dhasarathy

Roemmich

Claycombe

2017

Molecular Aspects of Medicine

View full text Add to dashboard Cite

Susceptibility to Apoptosis in Insulin-like Growth Factor-I Receptor-deficient Brown Adipocytes

Cited by 23 publications

References 69 publications

Insulin increases H2O2-induced pancreatic beta cell death

Insulin increases H2O2-induced pancreatic beta cell death

Adult neural stem cells expressing IL-10 confer potent immunomodulation and remyelination in experimental autoimmune encephalitis

Influence of maternal obesity, diet and exercise on epigenetic regulation of adipocytes

Contact Info

Product

Resources

About