Susceptibility testing of clinical isolates of Methylobacterium species

Brown, William J.; Sautter, Robert L.; Crist, Arthur E.

doi:10.1128/aac.36.8.1635

Cited by 26 publications

(17 citation statements)

References 9 publications

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“…In clinical health issues like treatments of infections, aminoglycosides and quinolones were reported to be effective (3,9), while public health issues have been countered with surfactants and sanitizers, like quaternary ammonium compounds (10). However, we found that Methylobacterium strains isolated from bathrooms were tolerant to various stresses, including surfactants like benzalkonium chloride (BAC) (8,11).…”

contrasting

confidence: 39%

Pentanol and Benzyl Alcohol Attack Bacterial Surface Structures Differently

Yano

Miyahara

Morii

et al. 2016

Appl Environ Microbiol

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bThe genus Methylobacterium tolerates hygiene agents like benzalkonium chloride (BAC), and infection with this organism is an important public health issue. Here, we found that the combination of BAC with particular alcohols at nonlethal concentrations in terms of their solitary uses significantly reduced bacterial viability after only 5 min of exposure. Among the alcohols, Raman spectroscopic analyses showed that pentanol (pentyl alcohol [PeA]) and benzyl alcohol (BzA) accelerated the cellular accumulation of BAC. Fluorescence spectroscopic assays and morphological assays with giant vesicles indicated that PeA rarely attacked membrane structures, while BzA increased the membrane fluidity and destabilized the structures. Other fluorescent spectroscopic assays indicated that PeA and BzA inactivate bacterial membrane proteins, including an efflux pump for BAC transportation. These findings suggested that the inactivation of membrane proteins by PeA and BzA led to the cellular accumulation but that only BzA also enhanced BAC penetration by membrane fluidization at nonlethal concentrations.

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contrasting

confidence: 39%

Pentanol and Benzyl Alcohol Attack Bacterial Surface Structures Differently

Yano

Miyahara

Morii

et al. 2016

Appl Environ Microbiol

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“…In agreement with a previous report, aminoglycosides were extremely active against Methylobacterium species in vitro, whereas â-lactam drugs showed variable inhibitory effects (Brown et al, 1992). Half of our isolates were susceptible to trimethoprim/sulfamethoxazole.…”

Section: Antibioticsupporting

confidence: 80%

Underdiagnosis of urinary tract infection caused by Methylobacterium species with current standard processing of urine culture and its clinical implications

Lee

Tang

Liu

2004

Journal of Medical Microbiology

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Methylobacterium species are environmental opportunistic bacteria, and urinary tract infection (UTI) caused by these pathogens has not yet been documented. Four cases of UTI with Methylobacterium bacteraemia in immunocompetent female patients are reported. Their urine cultures, processed according to standard procedures (i.e. incubation at 35 8C in ambient air for 24 h before incubation at room temperature for a further 24 h), were either negative or positive for Escherichia coli. Specially designed experiments indicated that colonies of Methylobacterium species were visualized on blood agar only after incubation at 35 8C for at least 40 h, and growth was completely suppressed when concurrently incubated with much smaller inocula of E. coli. The isolates were variably susceptible to cephalosporins, but 100 % susceptible to aminoglycosides. This study suggests an underdiagnosis of UTI caused by Methylobacterium species when the standard procedure of processing urine cultures is used, and implies that administration of aminoglycosides is important when treatment of UTIs with cephalosporin fails.

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“…Two large Methylobacterium studies assessing in vitro antibiotic susceptibility have demonstrated broad resistance to beta-lactam antibiotics (2,6). To date, isolates tested have been imipenem susceptible (1,7,9).…”

Section: Discordant Carbapenem Susceptibility In Methylobacterium Spementioning

confidence: 99%