Susceptibility Testing of Anaerobic Bacteria with 100-μg Carbenicillin Disks

Laslie, W. Wayne; Lambe, Dwight W.

doi:10.1128/aac.10.1.112

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…With some B. fragilis strains there was a slight turbidity in the drug-containing tubes, but it was easy to ascertain a marked difference from the heavily grown control tube without drug. Difficulties (7,16,18). The problems met in reading the diffusion tests were mainly cloudy zones due to the dense inoculum or organisms that grew well close to the disk but were inhibited further away from the disk (33).…”

Section: Resultsmentioning

confidence: 99%

“…Direct numerical comparisons of the extent of variability of the two test procedures cannot be made, because the results were obtained in two systems not directly transferable (7). DISCUSSION There is controversy over the questions of whether the susceptibility testing of anaerobic bacteria should be performed routinely or whether the management of anaerobic infections should rely upon published susceptibility patterns for the different bacterial speeies (11,16,26,34). I believe that routine testing should be done for clinically significant anaerobes from sources not contaminated with normal flora, since the susceptibility pattern of various anaerobes against several antimicrobial agents is known to change (12, 15-17, 21, 28, 30 (4, 23, 24, 28, 31, 32; additional data were provided by Hoffmann-La Roche and Co. AG and Pfizer AG).…”

Section: Resultsmentioning

confidence: 99%

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Susceptibility of Anaerobic Bacteria to Metronidazole, Ornidazole, and Tinidazole and Routine Susceptibility Testing by Standardized Methods

Wüst

1977

Antimicrob Agents Chemother

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A total of 114 strains ofanaerobic bacteria were examined for their susceptibility to metronidazole, ornidazole, and tinidazole by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration in different media. All strains, with the exception of the isolates ofPropionibacterium acnes, were inhibited by 3.1 ug each and killed by 6.3 ,ug each of all three nitroimidazole compounds per ml. No significant differences in MIC values were found among metronidazole, ornidazole, and tinidazole. Only minor differences were detected by comparing MIC values obtained in brain heart infusion agar with and without sheep blood, brucella agar, and Mueller-Hinton agar (both containing blood). When the strains were tested by the modified broth-disk method proposed by Metronidazole and related nitroimidazole derivatives, including ornidazole and tinidazole, are receiving increasing attention in the treatment of anaerobic infections. This interest rests on the results ofintensive in vitro susceptibility evaluations (2-4, 9, 10, 20, 24, 26, 28, 32) and limited but promising trials of these agents in patients infected with various anaerobic bacteria (14,19,31,39 Currently, there are several methods proposed for routine susceptibility testing ofanaerobic bacteria (5,8,21,25,29,36,37 Clostridium sporogenes, 1 Clostridium tertium, 1 Clostridium sp. The strains were maintained at room temperature in chopped-meat broth (13).Media. Throughout the experiments, prereduced, anaerobically sterilized media were used. These were prepared according to methods described by Holdeman and Moore (13). The basic media were supplemented brain heart infusion (BHI) broth and chopped-meat carbohydrate (CMC) broth. The basal peptone-yeast extract medium used for testing biochemical reactions contained 0.5% peptone (Difco) and 0.5% Trypticase (BBL) instead of 1% peptone (W. E. C. Moore, personal communication).The media used for MIC determinations were: BHI agar (Difco), supplemented with 0.5% yeast extract (Difco), with and without 5% citrated sheep blood (BHIA); brucella agar (kindly supplied by Pfizer Overseas, Inc., New York) with 5% sheep blood; Mueller-Hinton agar (BBL), supplemented with 0.5% yeast extract (Difco) and 5% sheep blood. All media for MIC determinations were supplemented with menadione (0.5 ,g/ml) and hemin (5 ,tg/ml).Antimicrobial agents. Metronidazole and ornidazole were kindly supplied by P. Angehrn, Hoffmann-La Roche & Co. AG, Basel, Switzerland, and tinidazole was supplied by E. Milek, Pfizer AG, Zurich, Switzerland.Stock solutions containing 1,000 ,ug of each antimicrobial agent per ml were prepared in distilled water, filter-sterilized (Millipore, 0.22 um), and kept frozen at -20°C.MIC determinations. MIC determinations were performed according to Blazevic (1). For the agar dilution technique, plates were prepared containing serial twofold dilutions of each antimicrobial agent from 100 to 0.1 ,ug/ml. The plates were held overnight at room temperature in GasPak jars (BBL) or prepared on the day of th...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Susceptibility of Anaerobic Bacteria to Metronidazole, Ornidazole, and Tinidazole and Routine Susceptibility Testing by Standardized Methods

Wüst

1977

Antimicrob Agents Chemother

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A Standardized Disk Method for Antibiotic Susceptibility Testing for Anaerobes; Four Years of Antibiotic Data

Lambe

Laslie

1980

Anaerobic Bacteria

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Brain abscess due to Streptococcus MG-intermedius (Streptococcus milleri)

Melo¹,

Raff²

1978

J Clin Microbiol

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Three cases of brain abscesses due to Streptococcus MG-intermedius are reported, and the literature pertaining to this subject is reviewed. The importance of careful and complete identification of these etiological agents of infection is stressed. The clinical presentation, the origin of S. MG-intermedius producing brain abscess, and its relation to hepatic abscesses and endocarditis are discussed.

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Susceptibility Testing of Anaerobic Bacteria with 100-μg Carbenicillin Disks

Abstract: A total of 245 strains ofanaerobic bacteria were examined for their susceptibility to

Cited by 3 publications

References 30 publications

Susceptibility of Anaerobic Bacteria to Metronidazole, Ornidazole, and Tinidazole and Routine Susceptibility Testing by Standardized Methods

Susceptibility of Anaerobic Bacteria to Metronidazole, Ornidazole, and Tinidazole and Routine Susceptibility Testing by Standardized Methods

A Standardized Disk Method for Antibiotic Susceptibility Testing for Anaerobes; Four Years of Antibiotic Data

Brain abscess due to Streptococcus MG-intermedius (Streptococcus milleri)

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