Susceptibility test of female anopheles mosquitoes to ten insecticides for indoor residual spraying (IRS) baseline data collection in Northeastern Nigeria

Umar, Aliyu M.; Kabir, B. G. J.; Amajoh, C. N.; Inyama, P.U.; Ordu, D A; Barde, Auwal Alhassan; Misau, A A; Sambo, M L; Babuga, U.; Kobi, M; Jabbdo, M A

doi:10.5897/jen2014.0100

Cited by 18 publications

(33 citation statements)

References 22 publications

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“…from the evaluated LGAs of Lagos State. Several previous studies in different parts of Nigeria have recorded similar resistant level to different Pyrethriods and DDT 7,9,11,12 . Pyrethriods resistance recorded in these study areas could be detrimental to the effectiveness of LLINs as they are the only class of insecticide currently approved for use in LLINs.…”

Section: Discussionmentioning

confidence: 70%

“…The effectiveness and efficacy of the few insecticides approved for the control of malaria vectors by WHO has been greatly hampered by the advent of insecticide resistance. In Nigeria, Pyrethriods and DDT resistance has been reported in several parts of the country [7][8][9][10][11][12] . Insecticides resistance in malaria vectors can result from one or combination of behavioral changes, morphological modifications, target site mutation and metabolism by detoxifying enzymes.…”

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confidence: 99%

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First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in Nigeria

Fagbohun

Idowu

Otubanjo

et al. 2020

Sci Rep

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First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in nigeria ifeoluwa Kayode fagbohun 1 ✉ , emmanuel taiwo idowu 1 , olubunmi Adetoro otubanjo 1 & taiwo Samson Awolola 2 Susceptibility and PBO synergist bioassays were done using 3-5 days old female Anopheles mosquito collected from Lagos State, Nigeria with WHO test papers DDT (4%), permethrin (0.75%), Bendiocarb (1%) and PBO (4%) according to standard procedures. The activities of cytochrome P450s, glutathione S-transferase and carboxylesterases were determined using biochemical assays. the presence of kdr-w, kdr-e and Ace-1 R mutations were examined using molecular assays. Resistance to DDt and permethrin in An gambiae s.s from the four Local Government Areas (LGAs) was recorded while suspected resistance to bendiocarb was recorded in mosquitoes from Alimosho and Kosofe LGAs. PBO synergist reduced the knockdown time and also recorded significantly (P < 0.05) higher 24 hrs percentage mortality compared to non-synergized bioassays. Increased activities of detoxifying enzymes was recorded in wild mosquito compared to the insecticides susceptible laboratory strain and this was significant (P < 0.05) in P450s, esterase α and β. Kdr-w was detected in An. gambiae s.s from all the LGAs, kdr-e (L1014S) was detected in Alimosho, Kosofe and ibeju-Lekki, while the Ace-1 R gene was detected in Alimosho and Kosofe. Results from this study provide evidence for resistance of An. gambiae from Lagos State to multiple classes of neurotoxic insecticides with multiple resistance mechanisms to these insecticides.

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Section: Discussionmentioning

confidence: 70%

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confidence: 99%

First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in Nigeria

Fagbohun

Idowu

Otubanjo

et al. 2020

Sci Rep

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“…The paucity of information on dominant vector species (DVS) is most extreme in the Sahelo-Sudanian region of the country. With the exception of few focal studies 7,8 reliable information on the DVS from northern Nigeria is grossly lacking for decades following the comprehensive study conducted in Garki 9 . This is in stark contrast to the situation in southern part of Nigeria (especially the south-west), where significant progress on malaria vector bionomics has been achieved 10–13 .…”

Section: Introductionmentioning

confidence: 99%

Temporal escalation of Pyrethroid Resistance in the major malaria vector Anopheles coluzzii from Sahelo-Sudanian Region of northern Nigeria

Ibrahim

Mukhtar

Datti

et al. 2019

Sci Rep

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Despite the highest global burden of malaria, information on bionomics and insecticide resistance status of malaria vectors is grossly lacking in the densely populated Sahelo-Sudanian region of Nigeria. To support evidence-based vector control we characterised transmission and resistance profiles of Anopheles coluzzii populations from three sites in northern Nigeria. High sporozoite infection (~19.51%) was found in the An . coluzzii populations. A high pyrethroid resistance was observed with only 1% mortality against deltamethrin, a high LD 50 (96.57 µg/ml), and a high LT 50 (170.27 min, resistance ratio of ~51 compared with the fully susceptible Ngoussou colony). Moderate carbamate resistance was observed. Synergist bioassays significantly recovered deltamethrin susceptibility implicating CYP450s (mortality = 85%, χ 2 = 134.04, p < 0.0001) and esterases (mortality = 56%, χ 2 = 47.31, p < 0.0001). Reduced bed net efficacy was also observed, with mortalities on exposure to the roof of PermaNet3.0 (PBO + deltamethrin) more than 22 times compared to the side panel (deltamethrin). TaqMan genotyping revealed a high frequency of 1014F kdr mutation (82%) with significant difference in genotype distribution associated with permethrin resistance [OR = 4.69 (CI:1.53–14.35, χ 2 = 8.22 p = 0.004]. Sequencing of exons 18–21 of the VGSC led to detection of two additional nonsynonymous mutations, Ile10148Asn and Ser1156Gly. These findings highlight the threats posed by the highly resistant An . coluzzii to malaria control in Nigeria.

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“…This implies that these pyrethriod treated nets have been in use till date in these areas. Previous studies revealed that use of LLINs could result in development of insecticide resistance in Anopheles mosquitoes to pyrethriods [9,23]. The possibility of these LLINs inducing changes in the adult mosquito populations thus contributing to the resistance status recorded in these study areas may not be ruled out.…”

Section: Discussionmentioning

confidence: 90%

Assessment of Oral Health Knowledge, Attitude and Practice for Public Primary School Teachers in the City of Madinah, Saudi Arabia

Al-Johani¹,

Elanbya²

2019

JAMMR

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Background: School teachers can play an important role in helping to instill good health habits in their students. Consequently, teachers need to have an adequate knowledge and a proper attitude regarding oral health. Method and Materials: This is a cross sectional study conducted among public primary school teachers in Al-Madinah city. A questionnaire was distributed in 10 randomly selected schools. All teachers were asked to participate. Data was analyzed using SPSS software. Results: 200 teachers were asked to participate all of them responded. 81% of the teachers were aware that caries are caused by bacteria in the oral cavity. 69.5% of teachers thinks that fluorides strength teeth. 44.5 % of the teachers brushed their teeth twice a day. Only 15% of the teachers regularly discuss oral health topics with their students. 74.5% of them think that it is necessary to treat tooth caries in primary teeth. 70.5% think that dental health education should be included in the primary school curriculum. 64% of them believed that schools should restrict the consumption of caries causing snacks during school hours. Conclusion: Teachers’ knowledge regarding oral health needs improvement. Their own oral health practices are fair; however, their attitude towards oral health is very positive.

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Susceptibility test of female anopheles mosquitoes to ten insecticides for indoor residual spraying (IRS) baseline data collection in Northeastern Nigeria

Abstract: Malaria is a major public health problem in

Cited by 18 publications

References 22 publications

First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in Nigeria

First report of AChE1 (G119S) mutation and multiple resistance mechanisms in Anopheles gambiae s.s. in Nigeria

Temporal escalation of Pyrethroid Resistance in the major malaria vector Anopheles coluzzii from Sahelo-Sudanian Region of northern Nigeria

Assessment of Oral Health Knowledge, Attitude and Practice for Public Primary School Teachers in the City of Madinah, Saudi Arabia

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