Susceptibility Test Methods: Fastidious Bacteria

Jorgensen, James H.

doi:10.1128/9781555816728.ch71

Cited by 18 publications

(17 citation statements)

References 262 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Test bacteria were grown in 100 mL BHI (Brain Heart Infusion, Sigma Aldrich Ltd., Hungary). Each bacteria were incubated in a shaker incubator (C25 Incubator Shaker, New Brunswick Scientific, Edison, New Jersey, United States) at 37°C and at a speed of 60 rpm for 12 h ( 41 ). The bacterial suspensions were diluted with clear BHI to the appropriate concentrations for each assay.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and Biofilm Degradation Effects of Hungarian Honeys Linked With Botanical Origin, Antioxidant Capacity and Mineral Content

et al. 2022

View full text Add to dashboard Cite

The aim of the study was to assess the impact of four unifloral honeys on the food-borne pathogens Pseudomonas aeruginosa and Staphylococcus aureus, by analyzing the honeys’ antibacterial and biofilm degradation effects, as well as their antioxidant activity and element content. Linden and milkweed honeys represented light colored honeys, while goldenrod and chestnut honeys the darker ones. The botanical origin of the honeys and the relative frequency of their pollen types were established with melissopalynological analysis. The antioxidant capacities were calculated by two single electron transfer based methods (TRC – Total Reducing Capacity and TEAC – Trolox Equivalent Antioxidant Capacity) and a hydrogen atom transfer based assay (ORAC – Oxygen Radical Absorbance). The amount of four main macro- and two microelements was quantified. The antibacterial activity was determined by minimum inhibitory concentration (MIC) and membrane degradation assays. Furthermore, the biofilm degradation power of the samples was studied as well. The light colored linden honey with the lowest TRC and TEAC, but with the highest ORAC antioxidant activity and high element content showed the best antibacterial and biofilm degradation effects. Meanwhile, the dark colored chestnut honey with significantly higher single electron transfer based antioxidant capacities, with high element content, but lower ORAC showed significantly higher MIC and lower membrane degradation activity than linden honey. In case of biofilm degradation, both honey types gave similarly high inhibitory effect. Goldenrod honey was similarly effective regarding its MIC properties like chestnut honey, but had significantly lower antioxidant potential and ability to disrupt bacterial membranes and biofilms. Milkweed honey was the honey type with the lowest bioactivity and element content. The honeys, unequivocally characterized by their antioxidant characters and element content, displayed different antibacterial and biofilm degradation effects. In addition, some honey traits were found to be good predictors of the antimicrobial potential of honeys: ORAC assay showed correlation with the MIC values of both bacteria, and strict correlation was found between the mineral content and the antibiofilm activity of the studied honeys. Our studies indicate that unifloral honeys, such as linden and chestnut honeys, are plant-derived products with great potential as antimicrobial agents in food preservation, exhibiting remarkable antibacterial activity against food-borne pathogens.

show abstract

Section: Methodsmentioning

confidence: 99%

Antibacterial and Biofilm Degradation Effects of Hungarian Honeys Linked With Botanical Origin, Antioxidant Capacity and Mineral Content

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A high index of suspicion and collection of appropriate anaerobic samples may increase the number of reported cases of A. naeslundii [25].…”

Section: Discussionmentioning

confidence: 99%

“…It is given in a high dosage over a prolonged period, because the infection has a tendency to recur. Most deepseated infections can be expected to respond to intravenous penicillin G, 10 to 20 million units/day given for 2 to 6 weeks, followed by an oral phenoxypenicillin in a dosage of 2 to 4 g/day [1,3,24,25].…”

Section: Discussionmentioning

confidence: 99%

Mediastinitis Due to <i>Actinomyces Naeslundii</i>

Cekovska

Kaftandzieva

Petrovska

et al. 2010

Macedonian Journal of Medical Sciences

View full text Add to dashboard Cite

A 48-year-old man with retrosternal chest pain and upper abdominal pain with propagation to the back, with clinical signs of nausea, dyspnea and dysphagia was referred to the Clinics of thoracic surgery. After esophagography, operative treatment was indicated. Left thoracotomy with mediastinothomy were performed. During the operation, a sample of pus was taken and sent for a microbiological examination at the Institute of Microbiology and Parasitology, Medical Faculty in Skopje. Standard microbiological procedures were used. The sample was cultured anaerobically on Schaedler agar and incubated for 48 hours at 37ºC. Oval, smooth colonies were observed. Gram stained smear revealed short branching Gram-positive filaments suspected for genus Actinomyces. For a definitive diagnosis and differentiation of the Actinomyces species, an automated VITEK system was used. Two weeks after treatment by ceftriaxon, metronidazol and analgetics, all clinical signs improved and patient was released in good physical condition.

show abstract

“…These agents are also used in empiric and preventive therapies for the treatment of BHS infections [3, 4]. However, recent studies have shown considerable changes in the susceptibility of BHS to erythromycin and clindamycin, although different resistance rates to these agents owing to geographical variation and investigators have been reported [5-7]. β-Lactam agents have been the treatment of choice for VGS infections; however, increase in the incidence of VGS with multidrug-resistance to penicillin and other agents, such as cephalosporins, macrolides, lincosamides, tetracycline, quinupristin-dalfopristin, and quinolones, has been reported [7, 8].…”

Section: Introductionmentioning

confidence: 99%

“…However, recent studies have shown considerable changes in the susceptibility of BHS to erythromycin and clindamycin, although different resistance rates to these agents owing to geographical variation and investigators have been reported [5-7]. β-Lactam agents have been the treatment of choice for VGS infections; however, increase in the incidence of VGS with multidrug-resistance to penicillin and other agents, such as cephalosporins, macrolides, lincosamides, tetracycline, quinupristin-dalfopristin, and quinolones, has been reported [7, 8]. Moreover, CLSI has recommended that VGS isolated from normally sterile body sites should be tested for penicillin susceptibility by using a minimum inhibitory concentration (MIC) method and interpretive criteria [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the MicroScan MICroSTREP Plus Antimicrobial Panel for Testing ß-Hemolytic Streptococci and Viridans Group Streptococci

Kim

Jang

et al. 2011

Ann Lab Med

View full text Add to dashboard Cite

BackgroundIn order to determine the clinical usefulness of the MicroScan (Siemens Healthcare Diagnostics, USA) MICroSTREP plus antimicrobial panel (MICroSTREP) for testing antimicrobial susceptibility of β-hemolytic streptococci (BHS) and viridans group streptococci (VGS), we compared the accuracy of MICroSTREP with that of the CLSI reference method.MethodsSeventy-five BHS and 59 VGS isolates were tested for antimicrobial susceptibility to ampicillin, penicillin, cefotaxime, meropenem, erythromycin, clindamycin, levofloxacin, and vancomycin by using MICroSTREP and the CLSI agar dilution method.ResultsThe overall essential agreement with regard to minimum inhibitory concentrations (MICs) (within ±1 double dilution) between MICroSTREP and the CLSI reference method was 98.2%, and categorical agreement (CA) was 96.9%. For the BHS isolates, the CA for erythromycin was 96.0%, whereas that for cefotaxime, meropenem, levofloxacin, and vancomycin (for ampicillin, penicillin, and clindamycin; 98.7%) was 100%. For the VGS isolates, the CA for penicillin was 84.7% and that for erythromycin, clindamycin, and vancomycin (for meropenem, 86.5%; for ampicillin, 88.1%; and for cefotaxime and levofloxacin, 96.6%) was 100%. All categorical errors of penicillin and ampicillin in the VGS isolates were minor.ConclusionsThe accuracy of MICroSTREP is comparable to that of the CLSI reference method, suggesting that this panel can be effective for testing antimicrobial susceptibility of BHS and VGS.

show abstract

Susceptibility Test Methods: Fastidious Bacteria

Cited by 18 publications

References 262 publications

Antibacterial and Biofilm Degradation Effects of Hungarian Honeys Linked With Botanical Origin, Antioxidant Capacity and Mineral Content

Antibacterial and Biofilm Degradation Effects of Hungarian Honeys Linked With Botanical Origin, Antioxidant Capacity and Mineral Content

Mediastinitis Due to <i>Actinomyces Naeslundii</i>

Evaluation of the MicroScan MICroSTREP Plus Antimicrobial Panel for Testing ß-Hemolytic Streptococci and Viridans Group Streptococci

Contact Info

Product

Resources

About