Susceptibility Rates of Pulmonary and Extrapulmonary 229 Mycobacterium tuberculosis Isolates to Major Antituberculous Drugs and Comparison of E test and Standard Proportion Methods

Abstract

A AB BS S TTR RA AC CT T O Ob b j je ec c t ti i v ve e: : Fol lo wing the an timy co bac te ri al re sis tan ce that has be en emer ging, and struggling the aga inst re sis tan ce has be co me as im por tant as ef fec ti ve tre at ment in tu ber cu lo sis con trol. The drug suscep ti bi lity test (DST) re sults of 229 M.tu ber cu lo sis (MTB) which had be en iso la ted on Lo wens te in-Jen sen (L-J) me di um from va ri o us cli ni cal samp les we re eva lu a ted. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : All iso la tes are ori gi nated from112 pul mo nary samp les and 117 ex tra pul mo nary samp les. Af ter de con ta mi na ti on and L-J cul tu re pro ce du res, all iso la tes we re iden ti fi ed as MTB comp lex by using con ven ti o nal bi oc he mi cal tests inc lu ding ni a cin, nit ra te, ca ta la se and ca ta la se tests imp le men ted at 68°C. DST was per for med by E test (AB Bi o disk, Swe e den) met hod for iso ni a zi d (INH), ri fam pi cin (RIF), et ham but hol (EMB) and strep tomy cin (SM) ac cording to ma nu fac tu rer ad vi ces. L-J pro por ti on met hod was per for med for 59 MTB iso la tes in ad di ti on to E test. R Re e s su ul lt ts s: : The ove rall re sis tan ce ra tes of INH and RIF we re 4% and resistance to SM was 2% and of EMB was 1%. The re sis tant iso la tes we re fo und ge ne rally in the iso la tes ori gi na ted from pul mo nary samp les. The re sis tan ce ra tes to INH and RIF we re 7%, to SM 3% and to EMB 2% for pul mo nary; and to INH, RIF and SM we re 1% and to EMB was 0% for ex tra pul mo nary iso la tes, res pec ti vely. INH and RIF re sis tan ce was signi fi cantly hig her in pul mo nary iso la tes (p< 0.05). In 59 MTB iso la tes, ag re e ment bet we en E test and L-J propor ti on was 95% for INH and EMB, 86% for RIF, and 85% for SM. C Co on nc c l lu u s si i o on n: : Ac cor ding to DST re sults, an timy co bac te ri al re sis tan ce ra tes in ex tra pul mo nary MTB iso la tes we re sig ni fi cantly lo wer than pul mo nary iso la tes. E test met hod which is known as a re a so nably fast test in com pa ri son with con ven ti o nal sus cep tibi liy tests may be used as a ra pid di ag nos tic test met hod for DST. Ho we ver, the re is a ne ed for furt her E test stu di es com pa ring stan dard met hods in mo re MTB iso la tes. K Ke ey y W Wo or rd ds s: : Myco bac te ri um tu ber cu lo sis; tu ber cu lo sis, mul tid rug-re sis tant; tu ber cu lo sis; mic ro bi al sen si ti vity tests Ö ÖZ ZE ET T A Am ma aç ç: : Or ta ya çık mak ta olan an ti mik ro bi yal di ren cin ar dın dan di ren ce kar şı mü ca de le tü ber kü loz kon tro lün de et ki li te da vi ka dar önem li ha le gel miş tir. Çe şit li kli nik ör nek ler den alı nan, Lo wens te in-Jensen (L-J) be si ye rin de izo le edi len 229 M. tu ber cu lo sis (MTB) 'un du yar lı lık test so nuç la rı de ğer len di ril di. G Ge e --r re eç ç v ve e Y Yö ön n t te em m l le er r: : Tüm izo lat la rın 112'si ak ci ğer ör nek le rin den ve 117'si ak ci ğer dı şı ör nek ler den mey da na gel miş tir. Kon ta mi n...