Susceptibility of Various Cell Lines to Rabies Virus

Mifune, Kumato; Makino, Yoshihiro; Mannen, Kazuaki

doi:10.2149/tmh1973.7.201

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…We constructed plasmids for the expression of N-terminally Flag-tagged P proteins from various strains, including a RABV fixed strain Nishigahara (Ni) (Ishikawa et al, 1989), a street strain 1088, which was originally isolated from a woodchuck in the USA and has high pathogenicity after peripheral infection in mice (Mifune et al, 1979) and three lyssaviruses LBV, MOKV and DUVV. Expression levels of all P proteins in the plasmid-transfected cells were comparable (Fig.…”

Section: Resultsmentioning

confidence: 99%

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

Masatani

Ozawa

Yamada

et al. 2016

Journal of General Virology

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The P protein of rabies virus (RABV) is known to interfere with the phosphorylation of the host IFN regulatory factor 3 (IRF-3) and to consequently inhibit type I IFN induction. Previous studies, however, have only tested P proteins from laboratory-adapted fixed virus strains, and to the best of our knowledge there is no report about the effect of P proteins from street RABV strains or other lyssaviruses on the IRF-3-mediated type I IFN induction system. In this study, we evaluated the inhibitory effect of P proteins from several RABV strains, including fixed and street virus strains and other lyssaviruses (Lagos bat, Mokola and Duvenhage viruses), on IRF-3 signalling. All P proteins tested inhibited retinoic acid-inducible gene-1 (RIG-I)-and TANK binding kinase 1 (TBK1)-mediated IRF-3-dependent IFN-b promoter activities. On the other hand, the P proteins from the RABV street strains 1088 and HCM-9, but not from fixed strains Nishigahara (Ni) and CVS-11 and other lyssaviruses tested, significantly inhibited I-kappa B kinase e (IKKe)-inducible IRF-3-dependent IFN-b promoter activity. Importantly, we revealed that the P proteins from the 1088 and HCM-9 strains, but not from the remaining viruses, interacted with IKKe. By using expression plasmids encoding chimeric P proteins from the 1088 strain and Ni strain, we found that the C-terminal region of the P protein is important for the interaction with IKKe. These findings suggest that the P protein of RABV street strains may contribute to efficient evasion of host innate immunity.

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Section: Resultsmentioning

confidence: 99%

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

Masatani

Ozawa

Yamada

et al. 2016

Journal of General Virology

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“…Mouse neuroblastoma cell lines, NA and Neuro-2a (N2a) cells, were maintained in Eagle's minimal essential medium (MEM) supplemented with 10% fetal calf serum (FCS). The street RABV strain, 1088, which was originally isolated from a rabid woodchuck in North America [ 16 ], was propagated in suckling mouse brains, and the supernatant from a 10% brain homogenate in phosphate-buffered saline (PBS) supplemented with 2% FCS was used [ 17 ]. The entire genome sequence of 1088 has been determined (GenBank Accession no.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Favipiravir (T-705) in Rabies Postexposure Prophylaxis

et al. 2015

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Rabies is a fatal encephalitis caused by rabies virus (RABV), and no antiviral drugs for RABV are currently available. We report for the first time the efficacy of favipiravir (T-705) against RABV in vitro and in vivo. T-705 produced a significant, 3–4 log10 reduction in the multiplication of street and fixed RABV strains in mouse neuroblastoma Neuro-2a cells, with half-maximal inhibitory concentrations of 32.4 µM and 44.3 µM, respectively. T-705 significantly improved morbidity and mortality among RABV-infected mice when orally administered at a dose of 300 mg/kg/day for 7 days, beginning 1 hour after inoculation. T-705 significantly reduced the rate of virus positivity in the brain. Furthermore, the effectiveness of T-705 was comparable to that of equine rabies virus immunoglobulin for postexposure prophylaxis. Collectively, our results suggest that T-705 is active against RABV and may serve as a potential alternative to rabies immunoglobulin in rabies postexposure prophylaxis.

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“… 15 Strain 1088 is a street virus strain that was isolated from a rabid woodchuck in North America. 17 High Five cells were maintained in Gibco Grace's Insect Medium (Thermo Fisher Scientific) supplemented with 10% fetal calf serum. Hairless mice (Hos: HR‐1, female, 6‐week‐old) were purchased from Hoshino Laboratory Animals, Ibaraki, Japan.…”

Section: Methodsmentioning

confidence: 99%

Validation of serum apolipoprotein A1 in rabies virus‐infected mice as a biomarker for the preclinical diagnosis of rabies

Yamada

Kuribayashi

Inomata

et al. 2021

Microbiology and Immunology

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Rabies is a type of acute fetal encephalitis caused by rabies virus (RABV). While it becomes incurable after symptom onset, it can be prevented by post-exposure prophylaxis (PEP) during the long incubation period. While preclinical diagnosis aids the appropriate PEP administration, it is mostly nonfeasible owing to the absence of viremia or a specific antibody response during the incubation period. Here, an attempt was made to identify a serum biomarker for the preclinical diagnosis of rabies. Using the serum from a mouse inoculated intramuscularly (i.m.) with 5 × 10 5 focusforming units (FFU) of recombinant RABV expressing red firefly luciferase (1088/ RFLuc) immediately before symptom onset, two-dimensional differential gel electrophoresis was conducted, followed by mass spectrometry, and it was confirmed that apolipoprotein A1 (ApoA1) was up-regulated. ELISA showed that the serum ApoA1 and specific antibody levels increased during the incubation period and on the day of symptom onset. Since a lower infectious dose can be used to induce the unstable and long incubation period generally observed in natural infection, the ApoA1 level in mice inoculated i.m. with 10 3 FFU of 1088/RFLuc was examined by monitoring viral dynamics using in vivo imaging. The serum ApoA1 and specific antibody levels wereThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Susceptibility of Various Cell Lines to Rabies Virus

Cited by 10 publications

References 21 publications

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

Contribution of the interaction between the rabies virus P protein and I-kappa B kinase ϵ to the inhibition of type I IFN induction signalling

Efficacy of Favipiravir (T-705) in Rabies Postexposure Prophylaxis

Validation of serum apolipoprotein A1 in rabies virus‐infected mice as a biomarker for the preclinical diagnosis of rabies

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