Susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol

Amorim, Crystiane Venditi Gomes do; Aun, Carlos Eduardo; Mayer, Márcia Pinto Alves

doi:10.1590/s1806-83242004000300012

Cited by 33 publications

(40 citation statements)

References 27 publications

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“…Chlorhexidine gluconate has been used in endodontics as an irrigation solution (15)(16)(17)(18)20) and as an intracanal medication (15,17,19). It is active against a wide range of microorganisms, such as Gram-positive and Gram-negative bacteria (1,(15)(16)(17)(18)(19)(20)(21). In addition to its low cytotoxicity and high antimicrobial activity, CHX has other properties that make its use suitable in endodontic treatments, such as the ability to interact with oral tissues (1,15,17,(20)(21) and to continue to be released for a long period of time (17,(20)(21).…”

Section: Discussionmentioning

confidence: 99%

“…It is active against a wide range of microorganisms, such as Gram-positive and Gram-negative bacteria (1,(15)(16)(17)(18)(19)(20)(21). In addition to its low cytotoxicity and high antimicrobial activity, CHX has other properties that make its use suitable in endodontic treatments, such as the ability to interact with oral tissues (1,15,17,(20)(21) and to continue to be released for a long period of time (17,(20)(21). CHX gel is an effective intracanal medication due to its broad antimicrobial spectrum (1,(15)(16)(17)(18)(19)(20)(21), which is in agreement with the present findings.…”

Section: Discussionmentioning

confidence: 99%

“…Pulp therapy in deciduous teeth is a complex treatment due to the instrumentation procedures, the complexity of the apical delta (1)(2)(3), the biological cycle of the deciduous teeth (1-2), physiological root resorption and rhizolysis (1,(3)(4) and long treatment sessions during which the child sometimes does not always cooperate (5)(6). Therefore, it is important to select an effective material for endodontic treatment of deciduous teeth to eliminate microorganisms from the root canal system using an appropriate set of procedures, such as biomechanical preparation of the root canal, irrigation with different solutions and the use of intracanal dressings.…”

Section: Introductionmentioning

confidence: 99%

“…An ideal root canal filling material for deciduous teeth must have several properties, such as being harmless to the periapical tissues and permanent teeth germs, resorbing readily if pressed beyond the apex, and being antiseptic (3)(4)(5)(6). Then, the success of the endodontic therapy is primarily dependent on the susceptibility of the infecting microorganism to the commonly used antimicrobial agents (1,2). For pulpectomy cases in deciduous teeth, over 90% of all dental schools in Brazil have adopted the root filling paste proposed by Guedes-Pinto et al (7), which is called GuedesPinto Paste (GPP) and is composed of iodoform, camphorated parachlorophenol (CMPC) and Rifocort ® (5-9).…”

Section: Introductionmentioning

confidence: 99%

“…No studies have attempted to modify GPP by removing CMPC and adding another substance with antimicrobial activity and low cytotoxicity. Chlorhexidine has been used in periodontology for more than 20 years due to its antimicrobial properties and low cytotoxicity (1,(15)(16)(17)(18)(19)(20)(21). It also can be used as an endodontic irrigation solution (15)(16)(17)(18)20) and as an intracanal medication (15,(19)(20).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial action of root canal filling pastes used in deciduous teeth

Vargas‐Ferreira¹,

Angonese²,

Friedrich³

et al. 2010

Rev. odonto ciênc. (Online)

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Purpose: This study assessed the antimicrobial effectiveness of an iodoform-based paste (Guedes-Pinto Paste, GPP) and a paste modified by the addition of a 2% chlorhexidine gluconate gel (CHX) to replace the camphorated parachlorophenol component of the original GPP.Methods: The antimicrobial action was tested against the following microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli and Bacillus subtilis. In the agar diffusion test, 18 Petri plates with 20 mL of BHIA were inoculated with 0.1 mL of the microbial suspensions. Paper disks were immersed in the experimental solutions for 1 min and were then placed over the BHIA surface in each agar plate. The plates were maintained for 1 h at room temperature and then incubated at 37ºC for 48 h. The diameters of the microbial inhibition halos were measured around the paper disks containing the substances. Data were analyzed using the Mann-Whitney U-test (α=0.05).Results: Antimicrobial action was observed for the GPP and CHX pastes, which presented absence of turbidity for almost all microorganisms. No statistically significant difference in the antimicrobial action was found between GPP and CHX.Conclusion: Both pastes present similar antimicrobial effectiveness against several microorganisms commonly found in endodontic infections in deciduous teeth.Key words: Pulpectomy; deciduous teeth; chlorhexidine ResumoObjetivo: Este estudo avaliou a ação antimicrobiana de pastas, uma à base de iodofórmio conhecida por Pasta Guedes-Pinto (PGP) e outra modificada pela adição de digluconato de clorexidina (CHX) a 2% em substituição ao paramonoclorofenol canforado da formulação original da PGP. Metodologia: A ação antimicrobiana das duas pastas foi testada contra Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli eBacillus subtilis. O método empregado foi o de Diluição em Meio Sólido, Difusão em Ágar. Dezoito placas de Petri com 20 mL de BHIA foram inoculados com 0,1 mL das suspensões microbianas. Discos de papel foram imersos nas soluções experimentais por 1 min e colocados sobre a superfície de BHIA em cada placa. As placas foram mantidas em temperatura ambiente por 1 h e então incubadas a 37ºC por 48 h. O diâmetro da inibição microbiana foi medida ao redor dos discos de papel. Os dados foram pelo teste U de Mann-Whitney (α=0,05).Resultados: A PGP teve ação bacteriostática contra todos os microrganismos e também bactericida exceto para Enterococcus faecalis e Bacillus subtilis. A CHX apresentou ação bacteriostática e bactericida contra todos os microrganismos. Não houve diferença estatística significante quanto à efetividade antimicrobiana entre as pastas avaliadas.Conclusão: Ambas as pastas apresentaram ação antimicrobiana contra quase todos os microrganismos encontrados em infecções endodônticas de dentes decíduos.

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