Susceptibility of rabbits to Treponema pallidum after infection with Mycobacterium bovis.

Graves, Stephen

doi:10.1136/sti.55.6.394

Cited by 4 publications

(2 citation statements)

References 21 publications

(11 reference statements)

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“…Attempts to protect rabbits against treponemal challenge by systemic or local activation of macrophages have yielded conflicting results. Several investigators have failed to demonstrate protection against treponemal infection after systemic activation of macrophages with Mycobacterium bovis BCG (8,9,27) or Propionibacterium acnes (3). Harris et al (11), on the other hand, stated in an early study that the nonspecific in vivo activation of macrophages in rabbits by lecithin or trypan blue causes the early resolution of progressing syphilitic testicular lesions.…”

Section: Discussionmentioning

confidence: 99%

Activation of macrophages by products of lymphocytes from normal and syphilitic rabbits

Lukehart¹

1982

Infect Immun

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The production of soluble macrophage-activating factors by lymphocytes from syphilitic and normal rabbits was examined. Culture supernatants of splenic lymphocytes cultured with Treponema pallidum antigens or concanavalin A were incubated with rabbit peritoneal macrophages in vitro. The macrophage monolayers were then washed and infected with log-phase Listeria monocytogenes. Activation of the macrophages by lymphocyte products was measured by the ability of the macrophages to resist intracellular multiplication of Listeria and thus survive infection. Macrophages incubated with supernatants of unstimulated lymphocytes or T. pallidum-stimulated lymphocytes from normal rabbits were unable to resist intracellular multiplication of Listeria. Specifically stimulated lymphocytes from syphilitic rabbits and mitogen-stimulated lymphocytes from both normal and syphilitic rabbits demonstrated a clear ability to produce soluble factors which conferred upon macrophages the ability to limit the intracellular growth of the bacteria. Antigen or mitogen alone was unable to activate the macrophages; the presence of lymphocyte products was required.

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Section: Discussionmentioning

confidence: 99%

Activation of macrophages by products of lymphocytes from normal and syphilitic rabbits

Lukehart¹

1982

Infect Immun

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“…Development of large lesions in the adjuvant and the E. coli control groups and the development of much smaller lesions in the immunized groups (TmpA, TmpB, and TmpC) provided evidence that sensitization to the treponemal antigen must be considered in part responsible for the altered course of infection. Borenstein et al(3) and other investigators(1,(8)(9)(10)30) reported that nonspecific stimulation of the immune system does not promote resistance to T.…”

mentioning

confidence: 99%

Immunization of guinea pigs with recombinant TmpB antigen induces protection against challenge infection with Treponema pallidum Nichols

et al. 1991

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Treponema pallidum-susceptible guinea pigs of strain C4D were immunized with recombinant T. pallidum antigens TmpA, TmpB, TmpC, and TmpA plus TmpB plus TmpC; with Escherichia coli membranes; or with adjuvant alone. Animals in groups of five received six immunizing injections, each of 100 micrograms of antigen incorporated in RIBI adjuvant. After the sixth immunization, all experimental and nonimmunized controls were intradermally challenged with 3 x 10(6) T. pallidum Nichols freshly extracted from infected rabbit testes. Although high titers of antitreponemal antibodies in the fluorescent-treponemal-antibody test or an enzyme-linked immunosorbent assay were evoked in all animals immunized with recombinant antigens, only guinea pigs receiving TmpB antigen demonstrated protection expressed by the development of significantly (P less than 0.01) smaller, atypical lesions of significantly (P less than 0.01) shorter duration and devoid of or containing fewer T. pallidum organisms than lesions in the remaining immunized and control animals.

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Latex bread technique for detection of the plaque-forming cell response to teratocarcinoma tumor antigens

Bagasra

Eppright

Damjanov

1983

Cancer Immunol Immunother

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A latex bead technique modified for measuring the plaque-forming cell (PFC) response to teratocarcinoma tumor antigens in syngeneic animals is described. With this method one can detect both the primary (IgM) and the secondary (IgG) immune response to tumor antigens. Optimal detection of the PFC response depends on the proper ratio of sheep red blood cells to latex beads and the dose of tumor cell antigen used for immunization. The presence of fetal calf serum interfered with immunization of animals and the coating of the latex beads with the tumor cell antigens. The plaques obtained in response to immunization with teratocarcinoma cell antigens varied in size, probably reflecting the complex immune response to more than one class of antigens on tumor cells.

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Susceptibility of rabbits to Treponema pallidum after infection with Mycobacterium bovis.

Cited by 4 publications

References 21 publications

Activation of macrophages by products of lymphocytes from normal and syphilitic rabbits

Activation of macrophages by products of lymphocytes from normal and syphilitic rabbits

Immunization of guinea pigs with recombinant TmpB antigen induces protection against challenge infection with Treponema pallidum Nichols

Latex bread technique for detection of the plaque-forming cell response to teratocarcinoma tumor antigens

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