Susceptibility of Pediococcus spp. to antimicrobial agents

Abstract

Aim: To investigate the susceptibility of Pediococcus species to antimicrobial agents. Methods and Results: The susceptibility to 14 antimicrobial agents of 31 genotypically distinct strains of six Pediococcus species was assessed by using Etests on ISO‐sensitest agar supplemented with horse blood. The species included were Pediococcus acidilactici, Pediococcus damnosus, Pediococcus dextrinicus, Pediococcus inopinatus, Pediococcus parvulus and Pediococcus pentosaceus. For several antimicrobial agents, some species were more susceptible than others. The two industrially important species, P. acidilactici and P. pentosaceus, differed with respect to erythromycin and trovafloxacin susceptibility, and in general both species had higher minimum inhibitory concentrations than the other species. In an erythromycin‐resistant P. acidilactici, an erythromycin resistance methylase B [erm(B)] gene was identified by PCR. Using a plasmid preparation from strain P. acidilactici 6990, a previously erythromycin‐sensitive Lactococcus lactis strain was made resistant. Transformants harboured a single plasmid, sized at 11·6 kb through sequence analysis. In addition, the erm(B) gene was identified within the plasmid sequence. Conclusions: The phenotypic test indicated the absence of acquired antimicrobial resistance genes in 30 of the strains. Significance and Impact of the Study: These results will help in selection of the best Pediococcus strains for use as starter cultures.

Antimicrobial activity, antibiotic susceptibility and virulence factors of Lactic Acid Bacteria of aquatic origin intended for use as probiotics in aquaculture

“…In this respect, Ammor et al [41] reported that pediococci are intrinsically resistant to the latter two antibiotics, as well as to glycopeptides (vancomycin and teicoplanin), streptomycin, ciprofloxacin and trimethoprim-sulphamethoxazole. Other authors proposed a MIC for tetracycline in pediococci ranging between 8 and 16 mg/L [42], or of 32 mg/L for oxytetracycline in P. pentosaceus [17]. The tetracycline breakpoints suggested for pediococci by EFSA are lower than the MICs observed in our work and others [17,42].…”

Isolation of Pediococcus acidilacticiKp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry

“…isolated from food are limited. Penicillin G, imipenem, gentamicin, netilmicin, erythromycin, clindamycin, rifampin, chloramphenicol, daptomycin, and ramoplanin are generally active against Pediococcus species [24-27]. However, susceptibility is thought to be species-dependent.…”

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

“…Rif r marking to recover introduced Lactobacillus strains from the indigenous GIT microbiota has been successfully used in chickens (23,43), ducks (16), pigs (22,39), mice (19,41), and humans (20,45), so a Rif r background was unexpected in this study. Rif MIC levels within several Lactobacillus and Pediococcus species have been reported to range between Յ0.016 and 4 g ml Ϫ1 (12,13). da Costa et al (10) investigated the level of antimicrobial resistance in enterococci in broiler feed and broiler feed ingredients and found 58 and 60%, respectively, of enterococcal isolates were resistant to 5-g Rif discs.…”