Susceptibility of non‐thymine containing DNA to four bacterial restriction endonucleases

Ito, Junetsu; Kawamura, Fujio; Duffy, John

doi:10.1016/0014-5793(75)81011-8

Cited by 18 publications

(2 citation statements)

References 19 publications

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“…Type II restriction endonucleases are affected by the sequences flanking their recognition sites (4-8, 17-24), by DNA conformation (25)(26)(27), and by base modification (2)(3)(4)(5)(6)17,20,24,(28)(29)(30)(31). EcoRI is, perhaps, the best understood of these enzymes.…”

Section: Discussionmentioning

confidence: 99%

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α-Putrescinylthymine and the sensitivity of bacteriophageφW-14 DNA to restriction endonucleases

Miller¹,

Wakarchuk²,

Warren³

1985

Nucl Acids Res

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The modified base alpha-putrescinylthymine (putT) in phi W-14 DNA blocks cleavage of the DNA by 17 of 32 Type II restriction endonucleases. The enzymes cleaving the DNA do so to widely varying extents. The frequencies of cleavage of three altered forms of the DNA show that putT blocks recognition sites either when it occurs within the site or when it is in a sequence flanking the site. The blocking is dependent on both charge and steric factors. The charge effects can be greater than the steric effects for some of the enzymes tested. All the enzymes cleaving phi W-14 DNA release discrete fragments, showing that the distribution of putT is ordered. The cleavage frequencies for different enzymes suggest that the sequence CAputTG occurs frequently in the DNA. Only TaqI of the enzymes tested appeared not to be blocked by putT, but it was slowed down. TaqI generated fragments are joinable by T4 DNA ligase.

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Section: Discussionmentioning

confidence: 99%

“…The modifying groups in those DNAs examined vary considerably in size, and the majority of them are uncharged (1)(2)(3)(4)(5)(6). The DNA of Bacillus subtilis phage SP15 is unusual in that 62% of its thymine (T) residues are replaced by phosphoglucuronated and glucosylated 5-(4',5'-dihydroxypentyl)uracil (hp U; 9,10).…”

Section: Introductionmentioning

confidence: 99%